The comet assay with 8 mouse organs: results with 39 currently used food additives

Sasaki, Yu; Kawaguchi, Satomi; Kamaya, Asako; Ohshita, Miyuki; Kabasawa, Kazumi; Iwama, Kayoko; Taniguchi, Kazuyuki; Tsuda, Shuji

doi:10.1016/s1383-5718(02)00128-6

Cited by 628 publications

(401 citation statements)

References 31 publications

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“…However, many ASs, including saccharin and aspartame, the so-called first generation sweeteners, have safety issues (Olney et al, 1996;Reuber, 1978;Van den Eeden et al, 1994). Although new generation sweeteners, such as acesulfame K (Mukherjee and Chakrabarti, 1997) and sucralose (Sasaki et al, 2002), have recently been approved, the potential risks to human health have not been firmly established. Over the past 3 decades, the potential hazard of carcinogenicity has been raised, but remains controversial (Weihrauch and Diehl, 2004).…”

Section: Introductionmentioning

confidence: 99%

Modified Apolipoprotein (apo) A-I by Artificial Sweetener Causes Severe Premature Cellular Senescence and Atherosclerosis with Impairment of Functional and Structural Properties of apoA-I in Lipid-Free and Lipid-Bound State

Jang

Jeoung

Cho

2011

Molecules and Cells

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Long-term consumption of artificial sweeteners (AS) has been the recent focus of safety concerns. However, the potential risk of the AS in cardiovascular disease and lipoprotein metabolism has not been investigated sufficiently. We compared the influence of AS (aspartame, acesulfame K, and saccharin) and fructose in terms of functional and structural correlations of apolipoprotein (apo) A-I and high-density lipoproteins (HDL), which have atheroprotective effects. Long-term treatment of apoA-I with the sweetener at physiological concentration (3 mM for 168 h) resulted in loss of antioxidant and phospholipid binding activities with modification of secondary structure. The AS treated apoA-I exhibited proteolytic cleavage to produce 26 kDa-fragment. They showed pro-atherogenic properties in acetylated LDL phagocytosis of macrophages. Each sweetener alone or sweetener-treated apoA-I caused accelerated senescence in human dermal fibroblasts. These results suggest that long-term consumption of AS might accelerate atherosclerosis and senescence via impairment of function and structure of apoA-I and HDL.

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Section: Introductionmentioning

confidence: 99%

Modified Apolipoprotein (apo) A-I by Artificial Sweetener Causes Severe Premature Cellular Senescence and Atherosclerosis with Impairment of Functional and Structural Properties of apoA-I in Lipid-Free and Lipid-Bound State

Jang

Jeoung

Cho

2011

Molecules and Cells

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“…These additives are used widely for various purposes, including preservation, coloring and sweetening. Some food additives, however, have been prohibited from use because of their toxicity (Sasaki et al 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, risk assessment largely depends on laboratory toxicity studies (Sasaki et al 2002). So, the aim of this research is to examine the genotoxic and cytotoxic effects of benzoic acid used as food preservatives in human lymphocytes by using the following assays; sister chromatid exchanges (SCEs), chromosomal aberrations (CAs) and micronucleus (MN) assay in vitro.…”

Section: Introductionmentioning

confidence: 99%

The in vitro genotoxicity of benzoic acid in human peripheral blood lymphocytes

2009

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The chromosomal aberration (CA), sister chromatid exchange (SCE) and micronucleus test (MN) were employed to investigate the in vitro effect of antimicrobial food additive benzoic acid on human chromosomes. Lymphocytes were incubated with various concentrations (50, 100, 200 and 500 lg/ mL) of benzoic acid. The results of used assays showed that benzoic acid significantly increased the chromosomal aberration, sister chromatid exchange and micronucleus frequency (200 and 500 lg/mL) without changing the pH of the medium in a dosedependent manner. Also this additive significantly decreased the mitotic index (MI) at the highest concentration for 24 h and 100, 200 and 500 lg/mL for 48 h. This decrease was dose-dependent as well. However, it did not effect the replication (RI) and nuclear division (NDI) indices.

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“…Os corantes: amarelo crepúsculo, azul brilhante e carmin não acarretaram danos estatisticamente significativos 32 .…”

Section: A Relação Entre Aditivos Alimentares E Neoplasiasunclassified

“…Ainda no mesmo estudo 32 , os aditivos conservadores (derivados do ácido benzóico e o nitrato de sódio) não provocaram danos no DNA. Já os antioxidantes BHA e butil hidroxitolueno (BHT)…”

Section: A Relação Entre Aditivos Alimentares E Neoplasiasunclassified

Consumo de aditivos alimentares e efeitos à saúde: desafios para a saúde pública brasileira

2009

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The comet assay with 8 mouse organs: results with 39 currently used food additives

Cited by 628 publications

References 31 publications

Modified Apolipoprotein (apo) A-I by Artificial Sweetener Causes Severe Premature Cellular Senescence and Atherosclerosis with Impairment of Functional and Structural Properties of apoA-I in Lipid-Free and Lipid-Bound State

Modified Apolipoprotein (apo) A-I by Artificial Sweetener Causes Severe Premature Cellular Senescence and Atherosclerosis with Impairment of Functional and Structural Properties of apoA-I in Lipid-Free and Lipid-Bound State

The in vitro genotoxicity of benzoic acid in human peripheral blood lymphocytes

Consumo de aditivos alimentares e efeitos à saúde: desafios para a saúde pública brasileira

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