The combination effect of auxin and cytokinin on in vitro callus formation of Physalis angulata L. – A medicinal plant

Mastuti, Retno; Munawarti, Aminatun; Firdiana, Elok Rifqi

doi:10.1063/1.5012721

Cited by 16 publications

(9 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…An excellent callus induction was observed at 3.5 and 3.0 mg L -1 2, 4-D after a culture period of 4-5 weeks [31]. The present results are in close conformity with Mastuti and coauthors [32] who reported more callus induction and growth in Physalis angulata when growth medium was fortified with 2,4-D and Kinetin as compared to IAA and NAA. Further, Kintzios and Skoula [33] also reported almost similar results, who investigated that a higher rate of callus induction was obtained when an intermediate auxin and cytokinin concentration were used for S. officinalis.…”

Section: Percent Callus Inductionsupporting

confidence: 90%

Callus Induction, Proliferation, Enhanced Secondary Metabolites Production and Antioxidants Activity of Salvia moorcroftiana L. as Influenced by Combinations of Auxin, Cytokinin and Melatonin

Bano

Khattak

Basıt

et al. 2022

Braz. arch. biol. technol.

View full text Add to dashboard Cite

Tissue culture technique is one of the best methods to reproduce salvia plant Therefore, the aim of this research was to enhance the in-vitro callus proliferation and production of secondary metabolites of S. moorcroftiana using different combinations of auxin, cytokinin and melatonin. Initially, callus induction was optimized using indole acetic acid (IAA), 2, 4-dichlorophenoxy acetic acid (2,4-D), and naphthalene acetic acid (NAA) applied at different concentrations in combination with 1 mg L -1 of 6-benzylaminopurine (BAP). The results indicates that earliest days to callus induction (14.67 days) was occurred in the media fortified HIGHLIGHTS• Tissue culture technique is considered to be one of the best methods to reproduce salvia plant.• Salvia is a medicinal plant with lots of chemical constituents.• Salvia plays an important role in treating different fatal diseases.• Maximum fresh and dry weights were obtained at 1.5 mg L -1 melatonin.• the highest DPPH scavenging activity, total phenolics and flavonoid content were obtained at 1.5 mg L -1 melatonin.

show abstract

Section: Percent Callus Inductionsupporting

confidence: 90%

Callus Induction, Proliferation, Enhanced Secondary Metabolites Production and Antioxidants Activity of Salvia moorcroftiana L. as Influenced by Combinations of Auxin, Cytokinin and Melatonin

Bano

Khattak

Basıt

et al. 2022

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…This effect is usually due to an increase in ethylene production at higher auxin concentrations (Morrish et al 1987;Vasil 1988;George et al 2008). According to the literature, the suitable growth regulators concentration and ratio for SE initiation (the "balance of concentration") should not be understood as the exogenous concentration of hormones used, but rather as a balance of the total concentration between endo-and exogenous hormones (Mastuti et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Pinus Sylvestris L. Mature Seeds Megamethophytes Cultured in Vitro. Influence of the Genotype and the Growth Regulators Content in Nutrient Medium on the Initiation of Callus Formation

Chirva

Ignatenko

Ershova

2022

Preprint

View full text Add to dashboard Cite

Despite the significant progress in forest biotechnology Scots pine still remains difficult for mass propagation via somatic embryogenesis (SE) due to the influence of many factors, such as the genotype and location of the donor plant, the composition of the nutrient medium, including the concentration of growth regulators, etc. Mature seeds were collected from Pinus sylvestris trees growing in the northern (Loukhsky district, Engozerskoye district forestry, natural phytocoenosis) and middle (Prionezhsky district, Petrozavodsk seed orchard) boreal subzone in Karelia (Russia). The influence of various growth regulators content in the composition of the DCR medium on the initiation of callus formation from mature seeds megagametophytes of both habitats was analyzed. Cytogenetic analysis of explants (root meristem of seedlings) showed that the frequency of mitosis pathologies is 4 times, and the proportion of cells with micronuclei is 6 times higher in P. sylvestris plants from a natural phytocoenosis than from Petrozavodsk seed orchard. Nevertheless explants collected from trees growing in the northern boreal subzone more often formed a cell culture (initiation frequency 13.4±2.9%) than megagametophytes from seed orchard (8.83±1.43%). The most active callus formation was registered on medium containing (1) 9 µM benzyladenine and 13.6 µM 2,4-dichlorophenoxyacetic acid; (2) 9 µM benzyladenine and 2.7 µM 1-naphthylacetic acid.

show abstract

“…Meanwhile, cytokinin growth regulators include BA, zeatin, and kinetin, each plays an important role in enhancing the cell division process, especially shoot regeneration, lateral growth, and production of multiple shoots. The ratio between cytokinin and auxin needs to be considered to determine growth direction [6]. The orchid subculture in this study originated from the crossing of Coelogyne pandurata and Coelogyne rumphii with various combinations of NAA and BA concentrations.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro

Hartati¹,

B.A²,

R.H³

et al. 2022

International Journal on Advanced Science, Engineering and Information Technology

View full text Add to dashboard Cite

The Orchid is an ornamental plant with high economic value due to the emergence of unique varieties. These varieties are produced from conventional breeding methods of crossing and selection. Meanwhile, the propagation of orchids through in vivo hatcheries is constrained because the seeds are too small to contain the endosperm. Tissue culture is an alternative for the propagation of orchids Efforts to propagate orchids through tissue culture is carried out by adding Growth Regulatory Substances such as auxins groups, namely NAA, IBA, IAA, and cytokinin groups, namely BA, BAP, kinetin, and TDZ to MS media, to enable the plants to take up adequate nutrients for growth. Therefore, this study aims to determine the effect of various concentrations of NAA and BA on the best ½ MS media for the growth of orchid subcultures. A completely randomized design pattern (CRD) of 2 factors with 12 treatment combinations and four replications were used, totaling 48 experimental units. The results showed that the addition of 1ppm NAA had the highest number of roots and length, while the addition of BA at 3ppm had the highest height, and the combination of 1ppm NAA and BA 3ppm treatment produced the highest number of leaves (42 sheets) and shoots. Based on the results, the addition of NAA above 3ppm followed by BA at 6ppm can produce more leaves and shoots, while NAA above 3ppm can produce higher plant height.

show abstract

The combination effect of auxin and cytokinin on in vitro callus formation of Physalis angulata L. – A medicinal plant

Cited by 16 publications

References 10 publications

Callus Induction, Proliferation, Enhanced Secondary Metabolites Production and Antioxidants Activity of Salvia moorcroftiana L. as Influenced by Combinations of Auxin, Cytokinin and Melatonin

Callus Induction, Proliferation, Enhanced Secondary Metabolites Production and Antioxidants Activity of Salvia moorcroftiana L. as Influenced by Combinations of Auxin, Cytokinin and Melatonin

Pinus Sylvestris L. Mature Seeds Megamethophytes Cultured in Vitro. Influence of the Genotype and the Growth Regulators Content in Nutrient Medium on the Initiation of Callus Formation

The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro

Contact Info

Product

Resources

About