Tissue culture technique is one of the best methods to reproduce salvia plant Therefore, the aim of this research was to enhance the in-vitro callus proliferation and production of secondary metabolites of S. moorcroftiana using different combinations of auxin, cytokinin and melatonin. Initially, callus induction was optimized using indole acetic acid (IAA), 2, 4-dichlorophenoxy acetic acid (2,4-D), and naphthalene acetic acid (NAA) applied at different concentrations in combination with 1 mg L -1 of 6-benzylaminopurine (BAP). The results indicates that earliest days to callus induction (14.67 days) was occurred in the media fortified
HIGHLIGHTS• Tissue culture technique is considered to be one of the best methods to reproduce salvia plant.• Salvia is a medicinal plant with lots of chemical constituents.• Salvia plays an important role in treating different fatal diseases.• Maximum fresh and dry weights were obtained at 1.5 mg L -1 melatonin.• the highest DPPH scavenging activity, total phenolics and flavonoid content were obtained at 1.5 mg L -1 melatonin.
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