The circulation of human astrovirus genotypes in the Central West Region of Brazil

Silva, Paula Andreia; Santos, Rodrigo Alessandro Tôgo; Costa, Paulo Sucasas; Teixeira, Jose M.; Giugliano, Loreny Gimenes; Andreasi, Márcia Sueli Assis; Leite, José Paulo Gagliardi; Schreier, Ecakart; Cardoso, Divina das Dôres de Paula

doi:10.1590/s0074-02762009000400021

Cited by 9 publications

(10 citation statements)

References 27 publications

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“…Three HAstVs genotypes co-circulated in Belém during the six months of study with HAstV-1 as the most prevalent 6,16,39,45 genotype (82.2%), as reported elsewhere . The concurrent circulation of other genotypes was observed in 6,10,36,39 various settings in Brazil as well as across the globe .…”

Section: Fromsupporting

confidence: 77%

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Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil

et al. 2010

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Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil Caracterização molecular de norovírus, sapovírus e astrovírus em crianças com gastroenterite aguda em Belém, Pará, Brasil Caracterización molecular de norovirus, sapovirus y astrovirus en niños con gastroenteritis aguda en Belém (Pará, Brasil) http://revista.iec.pa.gov.br Glicélia Cruz Aragão ABSTRACTThe importance of norovirus (NoVs), sapovirus (SaVs) and human astrovirus (HAstVs) as causes of gastroenteritis outbreaks is already well-defined, but a few studies have described sporadic cases of acute gastroenteritis caused by these viral entities. The aim of this study was to determine the role of these viruses in the etiology of acute gastroenteritis in children enrolled to participate in hospital -and emergency department -based intensive surveillance carried out in Belém, Brazil, from March to September 2003. A total of 305 stool specimens from patients with severe gastroenteritis were collected and screened by reverse transcription followed by polymerase chain reaction (RT-PCR), using the specific primers Mon 269 and Mon 270 for HAstVs, p289 and p290 for human calicivirus (HuCVs), and Mon 431/433 and Mon 432/434 for NoVs. Sequencing of RT-PCR HAstV, HuCV and NoV amplicons was carried out using the same primers. Of the 305 samples tested, 96 (31.5%) were positive, with 51 diagnosed as HuCVs, 40 as HAstVs and five as mixed infections. Of the 56 (18.4%) HuCVs sequenced, 30 were NoVs (9.8%) of genogroups and 15 (4.9%) were SaVs of types GI-1, GI-2 and GII-1. HAstVs, including genotypes 1, 8 and 2, were detected in 45 (14.7%) samples. This study has highlighted the importance of these viruses as causes of acute gastroenteritis and established the circulation of different genotypes during the study period. These results reinforce the need for establishing an intensive surveillance for gastroenteritis caused by these viruses to assess the burden of disease and to monitor the circulation of genotypes.

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confidence: 77%

“…The concurrent circulation of other genotypes was observed in 6,10,36,39 various settings in Brazil as well as across the globe . similar results were observed in Rio de Janeiro .…”

Section: Frommentioning

confidence: 95%

Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil

et al. 2010

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“…For example, RVAs have been predominantly detected among children up to 24 months of age (Camarota et al 1992, Cardoso et al 2003, Costa et al 2004, with a similar age range observed for astroviruses (Silva et al 2009) and adenoviruses (Cardoso et al 1989). The caliciviruses were detected in children up to 36 months of age (or 36 months-old) (Borges et al 2006).…”

Section: Discussionmentioning

confidence: 92%

“…In addition, in the Central-West Region of Brazil, many studies have focused on the investigation of other gastroenteric agents in this population (Cardoso et al 1989, 2003, Camarota et al 1992, Borges et al 2006, Oh et al 2006, Andreasi et al 2008, Silva et al 2009). Therefore, the objective of this study, the first conducted in this region, was the detection of HBoV-1 and HBoV-3 in faecal samples obtained from children under five years of age presenting with acute gastroenteritis.…”

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confidence: 99%

Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Sousa¹,

Souza²,

Fiaccadori³

et al. 2012

Mem. Inst. Oswaldo Cruz

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To determine the positivity rate of human bocavirus (HBoV) 1 and 3 among children who presented with acute gastroenteritis symptoms during the period of 1994-2004 in the Central-West Region of Brazil, 762 faecal samples were tested using polymerase chain reaction (PCR) for the detection of HBoV DNA. Primers for a segment of the non-structural viral protein 1 (NS1) gene of HBoV-1 and HBoV-3 were used. Twelve HBoV-positive samples were further characterised via genomic sequencing and phylogenetic analysis. Of the samples tested, 5.8% (n = 44) were positive for HBoV-1 or HBoV-3 and co-infection was observed in 14 (31.8%) of the 44 HBoV-positive samples. Nine of the 14 samples were also positive for Rotavirus A and five were positive for Aichi virus. The genomic sequencing of the NS1 partial sequence of 12 HBoV-samples showed that 11 samples were characterised as HBoV-1 and that one was characterised as HBoV-3. The phylogenetic analysis showed that the HBoV-1 samples had a high sequence homology to others previously identified in China, Sweden and Brazil. This is the first study conducted in the Central-West Region of Brazil to detect HBoV-1 and HBoV-3 in faecal samples from children with acute gastroenteritis. Further studies are required to define the role of HBoVs as aetiological agents of gastroenteritis

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“…Data from the literature show that the detection rates for these viruses among children with gastroenteritis in Brazil vary between 12 and 47% 4,14,[19][20][21] . However, there is no information available on the prevalence of these agents among the adult female population in Brazil.…”

Section: Resultsmentioning

confidence: 99%

Gastroenteric virus detection in fecal samples from women in Goiânia, State of Goiás, Brazil

Ferreira

Borges

Fiaccadori

et al. 2010

Rev. Soc. Bras. Med. Trop.

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Introduction: This was a prospective study that included women seen in the obstetrics and gynecology sector of Hospital das Clínicas, Federal University of Goiás, in Goiânia, State of Goiás, with the aim of detecting rotaviruses, adenoviruses, caliciviruses and astroviruses. Eighty-four women participated in the study and from these, 314 fecal samples were collected. Out of all of the women, 29 were seropositive for HIV and 55 were seronegative, and 45 and 39 were pregnant and non-pregnant, respectively. Methods: Fecal samples were collected from each woman once every two months over the period from July 2006 to June 2007, and they were screened for rotaviruses by means of polyacrylamide gel electrophoresis and immunoenzymatic assays, for caliciviruses and astroviruses by means of RT-PCR and for adenovirus by means of immunoenzymatic assays. The astroviruses were genotyped using nested PCR. Results: Among the 84 patients, 19 (22.6%) were positive for either calicivirus (14/19) or astrovirus (6/19), while one women was positive for both viruses in fecal samples collected on different occasions. Most of the positive samples were collected during the months of July and August (astrovirus) and September and October (calicivirus). None of the samples analyzed was positive for rotavirus or adenovirus. Gastroenteric viruses were detected in 13/19 (68.4%) of the pregnant women, whether HIV-seropositive or not. Conclusions: The results from the present study showed that neither pregnancy nor HIV-seropositive status among the women increased the risk of infection by any of the gastroenteric viruses studied. This study presents data on gastroenteric virus detection among pregnant and/or HIV-positive women.

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The circulation of human astrovirus genotypes in the Central West Region of Brazil

Abstract: Out of 1,588 faecal samples of children taken from three locations of the Central West Region of Brazil, 57 were positive for astroviruses (HAstVs) using reverse transcription-polymerase chain reaction (RT-PCR). They were genotyped by nested , , , and

Cited by 9 publications

References 27 publications

Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil

Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil

Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Gastroenteric virus detection in fecal samples from women in Goiânia, State of Goiás, Brazil

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