Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil

Abstract

Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil Caracterização molecular de norovírus, sapovírus e astrovírus em crianças com gastroenterite aguda em Belém, Pará, Brasil Caracterización molecular de norovirus, sapovirus y astrovirus en niños con gastroenteritis aguda en Belém (Pará, Brasil) http://revista.iec.pa.gov.br Glicélia Cruz Aragão ABSTRACTThe importance of norovirus (NoVs), sapovirus (SaVs) and human astrovirus (HAstVs) as causes of gastroenteritis outbreaks is already well-defined, but a few studies have described sporadic cases of acute gastroenteritis caused by these viral entities. The aim of this study was to determine the role of these viruses in the etiology of acute gastroenteritis in children enrolled to participate in hospital -and emergency department -based intensive surveillance carried out in Belém, Brazil, from March to September 2003. A total of 305 stool specimens from patients with severe gastroenteritis were collected and screened by reverse transcription followed by polymerase chain reaction (RT-PCR), using the specific primers Mon 269 and Mon 270 for HAstVs, p289 and p290 for human calicivirus (HuCVs), and Mon 431/433 and Mon 432/434 for NoVs. Sequencing of RT-PCR HAstV, HuCV and NoV amplicons was carried out using the same primers. Of the 305 samples tested, 96 (31.5%) were positive, with 51 diagnosed as HuCVs, 40 as HAstVs and five as mixed infections. Of the 56 (18.4%) HuCVs sequenced, 30 were NoVs (9.8%) of genogroups and 15 (4.9%) were SaVs of types GI-1, GI-2 and GII-1. HAstVs, including genotypes 1, 8 and 2, were detected in 45 (14.7%) samples. This study has highlighted the importance of these viruses as causes of acute gastroenteritis and established the circulation of different genotypes during the study period. These results reinforce the need for establishing an intensive surveillance for gastroenteritis caused by these viruses to assess the burden of disease and to monitor the circulation of genotypes.

Large gastroenteritis outbreak due to norovirus gii in São Paulo, Brazil, summer 2010

“…Thirty three (63.5%; 33/52) specimens positive for NoV displayed GII genogroup specificity by RT-PCR assay using the MON 431, 432, 433 and 434 primer pool 5 . The NoV GII detection agreed with previous works that describe a great incidence and distribution of this genogroup worldwide 7 , including Brazil 1,5 . Two samples were positive for GI (3.8%; 2/52) and one for GI/II (1.9%; 1/52), considered as sporadic cases.…”

Sapoviruses in Children With Acute Gastroenteritis From Manaus , Amazon Region, Brazil, 2010-2011

“…A research conducted in the state of Pará which is also located in the Amazon region, found a frequency a little higher (4.9%) among diarrheic children 17 . A recent study conducted in a day-care in Midwest Brazil detected SaVs in 4.6% of children, and the circulating genotypes were GI.1 and GI.3 19 .…”

“…Nevertheless, in Brazil, there are limited data, considering the small number of studies available 16 , 17 , 18 , 19 . Thus, the present study aimed to investigate the SaV occurrence among hospitalized children with acute gastroenteritis from Manaus, Amazonas State, and also to improve the data on the molecular epidemiology of this virus in Brazil.…”

Environmental Assessment of Classical Human Astrovirus in Uruguay

“…1 (Guix et al 2002;Malasao et al 2012;Medici et al 2012;De Grazia et al 2013). Moreover, the co-circulation with 1b and 1d lineages has been observed after a long period of 1a circulation as a unique lineage in South Korea and Brazil (Silva et al 2006;Gabbay et al 2007a;Aragão et al 2010;Jeong et al 2011). The 2d lineage of HAstV-2 genotype was the last proposed lineage when emerged in Italy in 2009 (Medici et al 2012), however, HAstV-2 strains that caused an outbreak of gastroenteritis in 2004 in an indigenous population at the southeast of the Brazil belonged to this lineage (Gabbay et al 2006).…”