The chitobiose-binding protein, DasA, acts as a link between chitin utilization and morphogenesis in Streptomyces coelicolor

Colson, Séverine; Wezel, Gilles P. van; Craig, Matthias; Noens, Elke E.; Nothaft, Harald; Mommaas, A. Mieke; Titgemeyer, Fritz; Joris, Bernard; Rigali, Sébastien

doi:10.1099/mic.0.2007/011940-0

Cited by 73 publications

(70 citation statements)

References 32 publications

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“…Abbreviations: nagA, GlcNAc-6-P deacetylase gene; nagB, GlcN-6-P isomerase gene; nagP, encoding sugar phosphotransferase EIIBC component for GlcNAc transport and phosphorylation; murQ, MurNAc-6-P etherase gene. Bacillus was generated with all YvoA-binding sequences upstream on Bacillus species nagA and nagP genes (see Table S1 in the supplemental material), and the Weblogo dre Streptomyces representation was generated with dre sites identified upstream of the S. coelicolor nagB, nagA, dasA (12), and PTS genes involved in GlcNAc uptake. molecular-weight effector(s) could modulate the DNA-binding capability of YvoA, the regulator was incubated with dre nagA in the presence of each of the four different aminosugar compounds GlcNAc, GlcNAc-6-P, GlcN-6-P, and GlcN.…”

Section: Resultsmentioning

confidence: 99%

Regulon of the N -Acetylglucosamine Utilization Regulator NagR in Bacillus subtilis

et al. 2011

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N-Acetylglucosamine (GlcNAc) is the most abundant carbon-nitrogen biocompound on earth and has been shown to be an important source of nutrients for both catabolic and anabolic purposes in Bacillus species. In this work we show that the GntR family regulator YvoA of Bacillus subtilis serves as a negative transcriptional regulator of GlcNAc catabolism gene expression. YvoA represses transcription by binding a 16-bp sequence upstream of nagP encoding the GlcNAc-specific EIIBC component of the sugar phosphotransferase system involved in GlcNAc transport and phosphorylation, as well as another very similar 16-bp sequence upstream of the nagAB-yvoA locus, wherein nagA codes for N-acetylglucosamine-6-phosphate deacetylase and nagB codes for the glucosamine-6-phosphate (GlcN-6-P) deaminase. In vitro experiments demonstrated that GlcN-6-P acts as an inhibitor of YvoA DNA-binding activity, as occurs for its Streptomyces ortholog, DasR. Interestingly, we observed that the expression of nag genes was still activated upon addition of GlcNAc in a ⌬yvoA mutant background, suggesting the existence of an auxiliary transcriptional control instance. Initial computational prediction of the YvoA regulon showed a distribution of YvoA binding sites limited to nag genes and therefore suggests renaming YvoA to NagR, for N-acetylglucosamine utilization regulator. Whole-transcriptome studies showed significant repercussions of nagR deletion for several major B. subtilis regulators, probably indirectly due to an excess of the crucial molecules acetate, ammonia, and fructose-6-phosphate, resulting from complete hydrolysis of GlcNAc. We discuss a model deduced from NagR-mediated gene expression, which highlights clear connections with pathways for GlcNAc-containing polymer biosynthesis and adaptation to growth under oxygen limitation.

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Section: Resultsmentioning

confidence: 99%

Regulon of the N -Acetylglucosamine Utilization Regulator NagR in Bacillus subtilis

et al. 2011

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“…The aminosugar GlcNAc is known to block Streptomyces development in the vegetative growth phase when added to the R2YE medium, thus leading to a nonsporulating (bald) phenotype. 20,23,24 We recently showed that GlcNAc supply drastically reduces siderophore biosynthesis through the direct transcriptional control of the gene for the iron utilization repressor DmdR1. 19,[25][26][27] Specifically, we showed that repression of DmdR1 by DasR, the central regulator of the GlcNAcmetabolic regulon, 17 was relieved in the presence of GlcNAc.…”

Section: S Coelicolor Bld Mutants Are Affected In Siderophore Biosynmentioning

confidence: 99%

Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

et al. 2014

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Streptomyces coelicolor is an important model organism for developmental studies of filamentous GCrich actinobacteria. The genetic characterization of mutants of S. coelicolor blocked at the vegetative mycelium stage, the so-called bald (bld) mutants that are unable to erect spore-forming aerial hyphae, has opened the way to discovering the molecular basis of development in actinomycetes. Desferrioxamine (DFO) production and import of ferrioxamines (FO; iron-complexed DFO) are key to triggering morphogenesis of S. coelicolor and we show here that growth of S. coelicolor on the reference medium for Streptomyces developmental studies is fully dependent on DFO biosynthesis. UPLC-ESI-MS analysis revealed that all bld mutants tested are affected in DFO biosynthesis, with bldA, bldJ, and ptsH mutants severely impaired in DFO production, while bldF, bldK, crr and ptsI mutants overproduce DFO.Morphogenesis of bldK and bldJ mutants was recovered by supplying exogenous iron. Transcript analysis showed that the bldJ mutant is impaired in expression of genes involved in the uptake of FO, whereas transcription of genes involved in both DFO biosynthesis and FO uptake is increased in bldK mutants. Our study allows proposing altered DFO production and/or FO uptake as a novel phenotypic marker of many S. coelicolor bld mutants, and strengthens the role of siderophores and iron acquisition in morphological development of actinomycetes.

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“…Similar to the pts genes, dasA is required for sporulation, and dasA deletion mutants have a bald phenotype. 111 Extracellular complementation experiments with different bld mutants showed that the dasR mutant is arrested at a very early stage of the developmental program. 109 A complete GlcNAc-dependent signaling model was recently proposed for Streptomyces by Nothaft et al 107 from the perspective of the environmental nutritional status to the onset of antibiotic production through DasR.…”

Section: Streptomycesmentioning

confidence: 99%

Carbon source regulation of antibiotic production

et al. 2010

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The chitobiose-binding protein, DasA, acts as a link between chitin utilization and morphogenesis in Streptomyces coelicolor

Cited by 73 publications

References 32 publications

Regulon of the N -Acetylglucosamine Utilization Regulator NagR in Bacillus subtilis

Regulon of the N -Acetylglucosamine Utilization Regulator NagR in Bacillus subtilis

Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

Carbon source regulation of antibiotic production

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