The Challenge of Global Emergence of Novel Colistin-Resistant <i>Escherichia coli</i> ST131

Moghadam, Majid Taati; Mirzaei, Mehrnaz; Moghaddam, Mozhdeh Fazel Tehrani; Babakhani, Sajad; Yeganeh, Omid; Asgharzadeh, Sajad; Farahani, Hamed Eraghiye; Shahbazi, Sepideh

doi:10.1089/mdr.2020.0505

Cited by 25 publications

(20 citation statements)

References 120 publications

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“…According to the literature, 20 , 21 ST131 and ST10 E. coli is one of the high-risk multidrug-resistant clones with a global distribution and the ability to survive and colonize in a variety of niches. In our study, IR596 and 19IR1045 isolate belongs to ST10 and ST131 colistin-resistant E. coli, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have reported that, E. coli ST131 is a great model organism to investigate the emergence of superbugs. 20 CTX-M-15-producing E. coli often belongs to a sequence type called ST131. 20 However, our study shows that this strain belongs to CTX-M-14-producing ST131 strain.…”

Section: Discussionmentioning

confidence: 99%

“… 20 CTX-M-15-producing E. coli often belongs to a sequence type called ST131. 20 However, our study shows that this strain belongs to CTX-M-14-producing ST131 strain. E. coli ST131 also showing high virulence and high antibiotic resistance.…”

Section: Discussionmentioning

confidence: 99%

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Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of blaNDM-5, blaCTX-M-65, blaOXA-10, blaTEM-1, and mcr-1.1 Genes Co-Occurrence in E. coli ST156

Zhang¹,

Fang²,

Zhang³

et al. 2022

IDR

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Background The emergence of carbapenem and colistin co-resistant Escherichia coli poses a huge challenge to infection control. The purpose of this study was to clarify the mechanism of the carbapenems and colistin co-resistance in E. coli strains. Methods Antimicrobial susceptibility test was carried out by agar dilution methods and colistin resistance was confirmed by broth microdilution methods. Whole genome sequencing was carried out, and resistance genes, sequence types and virulence genes of carbapenems and colistin co-resistance E. coli isolates were analyzed. Results The results showed that among the 176 carbapenem-resistant Enterobacteriaceae strains, 5 multidrug resistant E. coli strains exhibiting coresistance to carbapenem and colistin. The main mechanism of 5 E. coli strains in this study was generating carbapenem. Four E. coli strains were mcr- positive, while one mcr -negative strain had a new MgrB mutation. The bla NDM-5, bla CTX-M-65, bla OXA-10, bla TEM-1 and mcr-1.1 genes were simultaneously detected in E. coli 20IR1127 strain belonging to ST156 lineage. Other antimicrobial resistance genes encoding aminoglycosides-, sulfonamide-, chloramphenicol-, tetracyclines- and macrolides resistance were also detected. Conclusion The main mechanisms of carbapenem and colistin resistance were encoded by bla NDM and mcr1.1 , meanwhile mgrB mutations also contribute to colistin resistance. To our knowledge, this study is the first to report of E. coli ST156 strain in which the bla NDM-5 , bla CTX-M-65 , bla OXA-10 , bla TEM-1 and mcr1.1 genes coexist. In addition, there is also an E. coli ST457 strain, which carries bla TEM-1 , bla NDM-9 , bla CTX-M-199 and is positive for mcr1.1 gene.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of blaNDM-5, blaCTX-M-65, blaOXA-10, blaTEM-1, and mcr-1.1 Genes Co-Occurrence in E. coli ST156

Zhang¹,

Fang²,

Zhang³

et al. 2022

IDR

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“…In summary, the current study demonstrated once again the importance of E. coli O25b/ST131 clone as a type of clone capable of rapidly spreading and disseminate antibiotic resistance genes. Moreover, the study of various mechanisms in this clone type is useful to prevent the transmission of antimicrobial resistance genes, especially with the increasing rate of resistance to colistin among this clone [ 51 ]. Our study detected O25b/ST131 with a high resistance rate among clinical isolates of E. coli and ESBLs genes among them.…”

Section: Discussionmentioning

confidence: 99%

Molecular determination of O25b/ST131 clone type among extended spectrum β-lactamases production Escherichia coli recovering from urinary tract infection isolates

Khoshbayan

Zadeh

Moghadam

et al. 2022

Ann Clin Microbiol Antimicrob

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Background Escherichia coli (E. coli) O25b/ST131 clone causes urinary tract infection (UTI) and is associated with a broad spectrum of other infections, such as intra-abdominal and soft tissue infections, that can be affecting bloodstream infections. Therefore, since O25b/ST131 has been reported in several studies from Iran, in the current study, we have investigated the molecular characteristics, typing, and biofilm formation of O25b/ST131 clone type E. coli collected from UTI specimens. Methods A total of 173 E. coli isolates from UTI were collected. The susceptibility to all fourth generations of cephalosporins (cefazolin, cefuroxime, ceftriaxone, cefotaxime, ceftazidime, cefepime) and ampicillin, ampicillin-sulbactam and aztreonam was determined. Class A ESBLs, class D ESBL and the presence of pabB gene screenings to detect of O25b/ST131 clone type were performed by using of PCR. Biofilm formation was compared between O25b/ST131 isolates and non-O25b/ST131 isolates. Finally, ERIC-PCR was used for typing of ESBL positive isolates. Results Ninety-four ESBL positive were detected of which 79 of them were O25b/ST131. Antimicrobial susceptibility test data showed that most antibiotics had a higher rate of resistance in isolates of the O25b/ST131 clonal type. Biofilm formation showed that there was a weak association between O25b/ST131 clone type isolates and the level of the biofilm formation. ERIC-PCR results showed that E. coli isolates were genetically diverse and classified into 14 groups. Conclusion Our results demonstrated the importance and high prevalence of E. coli O25b/ST131 among UTI isolates with the ability to spread fast and disseminate antibiotic resistance genes.

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“…They can be used to counteract the antibiotic resistance. Due to the increasing prevalence of multiple drug resistant pathogens (MDR) and widespread drug resistance (XDR) and problems related to the treatment and management of diseases caused by "ESKAPE" pathogens ( Enterococcus faecium , Staphylococcus aureus, Klebsiella pnumoniae, Acinetobacter baumanni, Pseudomonas aeruginosa , and Enterobacter species), the development of new drugs is increasingly urgent 8 , 28 , 29 . Therefore, the use of diverse and newly endophytic actinobacteria isolated from particular habitats offers unique opportunities to extract new drugs and therapies.…”

Section: Discussionmentioning

confidence: 99%

Identification and antibacterial evaluation of endophytic actinobacteria from Luffa cylindrica

Mahdi

Bahrami

Kakaei

2022

Sci Rep

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The emergence of antibiotic-resistant bacteria has limited treatment options and led to the untreatable infections, thereby necessitating the discovery of new antibiotics to battel against bacteria. Natural products from endophytic actinobacteria (EA) serve as a reservoir for discovery of new antibiotics. Therefore, the current study focused on the isolation and antibacterial properties of EA isolated from Luffa cylindrica. Six strains were identified using morphological characterization, SEM analyses and 16S rRNA gene sequencing from the roots and leaves of the plant. They were taxonomically classified as Streptomycetaceae family. This is the first report on EA form L. cylindrica. The strains produced a chain of oval, cubed or cylindrical shaped spores with spiny or smooth surfaces. Three strains; KUMS-B3, KUMS-B4 and KUMS-B6 were reported as endophytes for the first time. Fifty percent of isolates were isolated from leaves samples using YECD medium. Our results showed that the sampling time and seasons may affect the bacterial diversity. All six strains had antibacterial activity against at least one of the tested bacteria S. aureus, P. aeruginosa, and E. coli. Among the strains, KUMS-B6 isolate, closely related to S. praecox, exhibited the highest antibacterial activity against both gram-positive and negative bacteria. KUMS-B6, KUMS-B5 and KUMS-B4 isolates strongly inhibited the growth of P. aeruginosa. Interestingly, the strains, isolated from leaves exhibited stronger antagonist activities compared to those isolated from the roots. The study revealed that the isolated strains from Luffa produce a plethora of bioactive substances that are potential source of new drug candidates for the treatment of infections.

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The Challenge of Global Emergence of Novel Colistin-Resistant Escherichia coli ST131

Cited by 25 publications

References 120 publications

Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of blaNDM-5, blaCTX-M-65, blaOXA-10, blaTEM-1, and mcr-1.1 Genes Co-Occurrence in E. coli ST156

Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of blaNDM-5, blaCTX-M-65, blaOXA-10, blaTEM-1, and mcr-1.1 Genes Co-Occurrence in E. coli ST156

Molecular determination of O25b/ST131 clone type among extended spectrum β-lactamases production Escherichia coli recovering from urinary tract infection isolates

Identification and antibacterial evaluation of endophytic actinobacteria from Luffa cylindrica

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