Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of blaNDM-5, blaCTX-M-65, blaOXA-10, blaTEM-1, and mcr-1.1 Genes Co-Occurrence in E. coli ST156

Zhang, Xiucai; Fang, Chi; Zhang, Junfeng; Wang, Hua; He, Rong; Zhou, Mingming

doi:10.2147/idr.s378574

Cited by 9 publications

(10 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Curiously, STs differed from continent to continent, and as frequent carriers for mcr -1 and bla NDM ( Lin et al, 2020 ; Zhang X. et al, 2022 ), more ARGs and VFs were observed within the ST156 strain prevailing in Asia and Europe, indicating that it represents a major risk to public health, highlighting the need for increased surveillance. For example, E. coli ST156, carrying bla NDM–5 , bla CTX–M–65 , bla OXA–10 , bla TEM–1 , and mcr -1, has been found in clinical isolates in China ( Lin et al, 2020 ; Zhang X. et al, 2022 ). Notably, ST648 has been reported as a high-risk, MDR, ESBL-producing strain in public aquatic environments ( Furlan et al, 2020 ), but ST648 was not frequently detected in our study, with almost all of them being in North America.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological and genomic characteristics of global mcr-positive Escherichia coli isolates

et al. 2023

View full text Add to dashboard Cite

ObjectiveThe worldwide dissemination of colistin-resistant Escherichia coli (E. coli) endangers public health. This study aimed to better understand the global genomic epidemiology of E. coli isolates carrying mobilized colistin resistance (mcr) genes, providing information to assist in infection and prevention.MethodsEscherichia coli genomes were downloaded from NCBI, and mcr was detected using BLASTP. Per software was used to extract information on hosts, resources, collection data, and countries of origin from GenBank. Sequence types (STs), prevalence of plasmids, antimicrobial resistance genes (ARG), and virulence factors (VF) in these genomes were analyzed. Statistical analyses were performed to assess the relationships between mcr, ARGs, plasmids, and STs.ResultsIn total, 778 mcr-positive isolates were identified. Four mcr variants were detected, with mcr-1 (86.1%) being the most widespread, followed by mcr-9 (5.7%), mcr-5 (4.4%), and mcr-3 (3.0%). Multiple ARGs were identified, with blaCTX–M (53.3%), fosA (28.8%), qnr (26.1%), blaNDM (19.8%), and aac (6’)-Ib-cr (14.5%) being the most common. Overall, 239 distinct STs were identified, of which ST10 (13.8%) was the most prevalent. A total of 113 different VFs were found, terC (99.9%) and gad (83.0%) were most frequently detected. Twenty types of plasmids were identified; IncFIB (64.1%), IncX (42.3%), and IncX (42.3%) were the most common replicons. IncI2 and IncX4 were frequently detected in mcr-1-positive isolates, whereas IncFII, IncI1-I, and IncHI2 were dominant plasmids in mcr-3, mcr-5, and mcr-9-positive isolates, respectively. A higher frequency of ARGs and VFs was observed among ST156 and ST131 isolates.ConclusionOur data indicated that more than half of the mcr-positive E. coli strains carried endemic ARGs and VFs. ST10 and ST156 isolates deserved further attention, given the rapid transmission of ST10 and the convergence of ARGs and VFs in ST156.

show abstract

Section: Discussionmentioning

confidence: 99%

Epidemiological and genomic characteristics of global mcr-positive Escherichia coli isolates

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Recently, the co-carriage of multiple RTs among a single E. coli strain has been frequently reported. For instance, a single E. coli strain from children in China co-carried with bla NDM−5 , bla CTX−M−65 , and mcr-1.1 [12], while E. coli strains from northeast India acquired 16S rRNA methyltransferases (16S-RMTase) along with bla CTX−M−15 , bla NDM , and bla OXA−48 [13]. Whole-genome sequencing (WGS) is a useful tool for studying the molecular epidemiology of pathogens from human, animal, and environmental sources.…”

Section: Introductionmentioning

confidence: 99%

Genomic characteristics of clinical carbapenem-resistant Escherichia coli isolates in a tertiary hospital, 2013–2021

Xie

Han

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Genomic characterization of carbapenem-resistant Escherichia coli (CREC) isolates is necessary to understand its transmission and implement infection control measures, given its potential threat to public health. Method：A total of 100 CREC isolates collected from 2013–2021 at a Nanjing tertiary hospital were analyzed using whole-genome sequencing (WGS). The distribution of resistant determinants (RTs), virulence factors (VFs), multi-locus sequence type (MLST), and FimH types was determined. Furthermore, a phylogenetic tree was constructed to investigate the genetic relationships. Result:All CREC strains (n=100) carried at least one carbapenem hydrolyzing ß-lactamase (CHßL)-encoding gene, with blaNDM (n=83, 83.0%) being the most common. Multiple RTs were detected with blaCTX (n=83, 83.0%) and blaTEM (n=78, 78.0%) being the most dominant ones. MLST identified 37 distinct STs, with ST167 (n=18, 18.0%) and ST410 (n=13, 13.0%) being the most predominant clones. Multiple plasmid replicons were found. The strains displayed 64 different virulence factors, with terC (n=98, 98.0%) and traT (n=76, 76.0%) being the most frequent. Genetic diversity was observed between the 100 CREC isolates, with clonal dissemination of ST167, ST361, ST2179, ST410, and ST131 being observed. Conclusion: The prevalence of blaNDM-5, a major blaCHßLs, is concerning due to its distribution of various co-carried resistance genes and their potential to spread through multiple plasmid replicons. The isolates exhibit genetic diversity based on STs, Fim typing, serotyping, and phylogenetic tree analysis. ST167, ST361, ST2179, ST410, and ST131 are linked to clonal dissemination, highlighting the need for effective infection control measures to prevent their spread.

show abstract

“…Co-transmission of CTX-M-65 with other ESBL-ARGs in E. coli have also been reported more frequently, ultimately leading to an increased risk of livestock-to-human transmission and global multidrug resistance (MDR) through horizontal gene transfer (HGT) [ 11 – 14 ]. Note that bla CTX-M-65 gene, with an elevated detection rate, has been reported in food animals [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Tn3-like structures co-harboring of blaCTX-M-65, blaTEM-1 and blaOXA-10 in the plasmids of two Escherichia coli ST1508 strains originating from dairy cattle in China

Wang,

Wei,

Zhu

et al. 2023

BMC Vet Res

View full text Add to dashboard Cite

The purpose of this study was to determine the level of horizontal transmission of the blaCTX-M-65 gene and the role of its associated mobile genetic elements (MGEs) in the bovine-derived Escherichia coli. After PCR identification, two plasmids carrying blaCTX-M-65 were successfully transferred to the recipient E. coli J53 Azr through conjugation assays and subsequently selected for Whole-Genome sequencing (WGS) analysis. The resistance profiles of these two positive strains and their transconjugants were also determined through antimicrobial susceptibility tests. Whole genome data were acquired using both the PacBio sequencing platform and the Illumina data platform. The annotated results were then submitted to the Genbank database for accession number recording. For comparison, the genetic environment of plasmids carrying the resistance gene blaCTX-M-65 was mapped using the Easyfig software. WGS analysis revealed Tn3-like composite transposons bearing blaCTX-M-65, blaTEM-1, and blaOXA-10 in the IncHI2-type plasmids of these two E. coli ST1508 strains. A phylogenetic tree was generated from all 48 assembled E. coli isolates blaCTX-M-65, blaTEM-1, and blaOXA-10 from the NCBI Pathogen Detection database with our two isolates, showing the relationships and the contribution of SNPs to the diversity between genetic samples. This study suggests that the transmissibility of blaCTX-M-65 on Tn3-like composite transposons contributes to an increased risk of its transmission in E. coli derived from dairy cattle.

show abstract

Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of blaNDM-5, blaCTX-M-65, blaOXA-10, blaTEM-1, and mcr-1.1 Genes Co-Occurrence in E. coli ST156

Cited by 9 publications

References 22 publications

Epidemiological and genomic characteristics of global mcr-positive Escherichia coli isolates

Epidemiological and genomic characteristics of global mcr-positive Escherichia coli isolates

Genomic characteristics of clinical carbapenem-resistant Escherichia coli isolates in a tertiary hospital, 2013–2021

Tn3-like structures co-harboring of blaCTX-M-65, blaTEM-1 and blaOXA-10 in the plasmids of two Escherichia coli ST1508 strains originating from dairy cattle in China

Contact Info

Product

Resources

About