Molecular determination of O25b/ST131 clone type among extended spectrum β-lactamases production Escherichia coli recovering from urinary tract infection isolates

Abstract

Background Escherichia coli (E. coli) O25b/ST131 clone causes urinary tract infection (UTI) and is associated with a broad spectrum of other infections, such as intra-abdominal and soft tissue infections, that can be affecting bloodstream infections. Therefore, since O25b/ST131 has been reported in several studies from Iran, in the current study, we have investigated the molecular characteristics, typing, and biofilm formation of O25b/ST131 clone type E. coli collected from UTI specimens. Methods A total of 173 E. coli isolates from UTI were collected. The susceptibility to all fourth generations of cephalosporins (cefazolin, cefuroxime, ceftriaxone, cefotaxime, ceftazidime, cefepime) and ampicillin, ampicillin-sulbactam and aztreonam was determined. Class A ESBLs, class D ESBL and the presence of pabB gene screenings to detect of O25b/ST131 clone type were performed by using of PCR. Biofilm formation was compared between O25b/ST131 isolates and non-O25b/ST131 isolates. Finally, ERIC-PCR was used for typing of ESBL positive isolates. Results Ninety-four ESBL positive were detected of which 79 of them were O25b/ST131. Antimicrobial susceptibility test data showed that most antibiotics had a higher rate of resistance in isolates of the O25b/ST131 clonal type. Biofilm formation showed that there was a weak association between O25b/ST131 clone type isolates and the level of the biofilm formation. ERIC-PCR results showed that E. coli isolates were genetically diverse and classified into 14 groups. Conclusion Our results demonstrated the importance and high prevalence of E. coli O25b/ST131 among UTI isolates with the ability to spread fast and disseminate antibiotic resistance genes.

Molecular Characterization of Extended Spectrum β-Lactamase-Producing Escherichia coli: Insights into the O25b-ST131 Clone in Mexican Urinary Tract Infections

“…Of the 86 isolates, 73% showed a weak relationship; however, the rest of the isolates were not related, indicating the absence of specific outbreaks due to the presence of multiple groups. The aforementioned findings are comparable to those in Iran, where 14 groups were identified in the clonal relationship analysis of uropathogenic E. coli (28). Similarly, a study in Romania identified 13 groups with a 75% similarity; nevertheless, Ecuador exhibited extensive genetic diversity among these strains, resulting in more than 30 groups (29,30).…”

Multidrug-Resistant and Extensively Drug-Resistant Escherichia coli in Sewage in Kuwait: Their Implications

“…This clone carries the β-lactamase gene, bla CTX-M [59]. In addition, some strains might carry bla TEM [60,61], bla SHV [61,62], ampC [60], or other genes [60]. In the strains of this clone, CTX-M is usually coproduced with OXA-1 and an aminoglycoside-modifying enzyme encoded by the aac(6 )-Ib-cr gene that confers resistance to aminoglycosides and fluoroquinolones [55,63].…”

Nitrofurantoin: properties and potential in treatment of urinary tract infection: a narrative review

“…Currently, 150 million Urinary Tract Infections (UTIs) are reported annually worldwide, and drug-resistant infections usually require more complex treatment regimens and are more likely to occur if treatment fails ( Khoshbayan et al., 2022 ). NF is outstanding because NF is most extensively utilized in humans, and its effectiveness in the treatment of lower UTIs and prophylaxis is well established ( Conklin, 1978 ).…”