The certified total glucosinolate content in three rapeseeds (CRMs 190, 366 and 367)

Wathelet, Jean‐Paul; Wagstaffe, P. J.; Boenke, Achim; Marlier, M.; Séverin, M.

doi:10.1007/bf00635464

Cited by 17 publications

(18 citation statements)

References 7 publications

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“…APCI‐MS and ESI‐MS have been applied for the analysis of desulfoglucosinolates and intact glucosinolates, respectively. The determination of desulfoglucosinolates using APCI has been recommended as an official reference method in the European Community 37. Generally, APCI is more applicable for relatively non‐polar analytes, while ESI is better for the ionization of polar and easily vaporized compounds.…”

Section: Resultsmentioning

confidence: 99%

Rapid screening method for intact glucosinolates in Chinese medicinal herbs by using liquid chromatography coupled with electrospray ionization ion trap mass spectrometry in negative ion mode

Lee

Chan

Liang

et al. 2008

Rapid Comm Mass Spectrometry

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An optimized method using liquid chromatography coupled with electrospray ionization ion trap mass spectrometry (LC/ESI-ITMS) in negative ion mode has been developed for screening different structural classes of intact glucosinolates in six Chinese medicinal herbs. The glucosinolates were extracted with hot methanol/water (70:30 v/v) and separation of the individual glucosinolates was achieved using a reversed-phase C18 column with an aqueous ammonium acetate/methanol gradient. Identification of the intact glucosinolates was based on the detection of compounds with a constant neutral loss of 242 Da corresponding to the combined loss of anhydroglucose (162 Da) and sulfur trioxide (80 Da) in collision-induced dissociation. The structures of the identified glucosinolates were confirmed with the use of group-specific product ions at m/z 195, 241, 259, 275 in their corresponding MS/MS product ion spectra. Differentiation of intact glucosinolates was achieved through their respective retention times and molecular masses as well as the characteristic product ions. The limits of detection were at the low nanogram level per injection, based on constant neutral loss scans. Significant variation in the compositions of intact glucosinolates was identified in the cruciferous herbs. This method was applied in the differentiation and quality control of two pairs of easily confused herbs.

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Section: Resultsmentioning

confidence: 99%

Rapid screening method for intact glucosinolates in Chinese medicinal herbs by using liquid chromatography coupled with electrospray ionization ion trap mass spectrometry in negative ion mode

Lee

Chan

Liang

et al. 2008

Rapid Comm Mass Spectrometry

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“…After analysis, the system was equilibrated for 10 min. The chromatograms were compared to the desulfo‐glucosinolate profiles provided by three certified reference materials recommended by the European Union and ISO (CRMs 366, 190 and 367) and using a pure standard GLS for the glucosativin …”

Section: Methodsmentioning

confidence: 99%

An approach to the phytochemical profiling of rocket [Eruca sativa (Mill.) Thell]

et al. 2013

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“…Glucosinolate concentrations were measured using a previously described method (Wathelet and others 1991; Hanlon and others 2007). A total of 200 mg of dry radish material were extracted 2 times with 2 mL of boiling 70% methanol.…”

Section: Methodsmentioning

confidence: 99%

Phytochemical Composition and Biological Activity of 8 Varieties of Radish (Raphanus sativus L.) Sprouts and Mature Taproots

Hanlon¹,

Barnes²

2011

Journal of Food Science

111

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Radishes (Raphanus sativus L.) are members of the cruciferous vegetable family that contain many classes of biologically active phytochemicals. This study determined the phytochemical composition of the sprouts and mature taproots of 8 radish varieties. Radish sprouts contained significantly greater concentrations of glucosinolates (3.8-fold) and isothiocyanates (8.2-fold) than the mature radish taproot and also contained significantly greater concentrations of phenolics (on average 6.9-fold). The anthocyanin concentrations of the mature radish taproot were significantly greater than in the sprouts of red, pink, and purple varieties. The primary anthocyanidins present in the red and pink radish varieties were pelargonidin and delphinidin, while the primary anthocyanidin in the purple radish variety was cyanidin. Radish sprouts were between 9- and 59-fold more potent than the corresponding mature taproot at activating the antioxidant response element (ARE) in a stably transfected hepatoma cell line. The ARE activity of the radish sprouts and mature taproots was significantly correlated with the total isothiocyanate concentration of the radishes. Practical Application: Understanding the influence variety and developmental stage has on the biological activity of cruciferous vegetables provides important information for further studies examining the in vivo effects of radish treatment and foundation for providing recommendations to reduce the risk of chronic disease through dietary intervention.

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The certified total glucosinolate content in three rapeseeds (CRMs 190, 366 and 367)

Cited by 17 publications

References 7 publications

Rapid screening method for intact glucosinolates in Chinese medicinal herbs by using liquid chromatography coupled with electrospray ionization ion trap mass spectrometry in negative ion mode

Rapid screening method for intact glucosinolates in Chinese medicinal herbs by using liquid chromatography coupled with electrospray ionization ion trap mass spectrometry in negative ion mode

An approach to the phytochemical profiling of rocket [Eruca sativa (Mill.) Thell]

Phytochemical Composition and Biological Activity of 8 Varieties of Radish (Raphanus sativus L.) Sprouts and Mature Taproots

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