The Bt gene cry2Aa2 driven by a tissue specific ST-LS1 promoter from potato effectively controls Heliothis virescens

Zaidi, Mohsin Abbas; Mohammadi, Mojtaba; Postel, Sandra; Masson, Luke; Altosaar, Illimar

doi:10.1007/s11248-004-7714-3

Cited by 25 publications

(14 citation statements)

References 32 publications

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“…Such a wide range of transgenic expression of cry1Ab gene in our study might be the result of these factors individually or in combination (Cheng et al 1998;Maqbool and Christou 1999;Zaidi et al 2005). The majority of transgenic lines with 0.045% Bt-toxin content showed 100% larval mortality.…”

Section: Discussionmentioning

confidence: 57%

Inheritance and field performance of transgenic Korean Bt rice lines resistant to rice yellow stem borer

Kim

et al. 2008

Euphytica

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Transgenic Korean rice plants containing the cry1Ab gene were developed for resistance against yellow stem borer (Scirpophaga incertulas, YSB). More than 100 independent transgenic lines from three Korean varieties (P-I, P-II and P-III) were generated. The amount of Cry1Ab in transgenic T 0 plants was as high as 2.88% of total soluble proteins. These levels were suYcient to cause 100% mortality of YSB larvae. The majority of T 1 transgenic lines originated from the varieties P-I and P-II followed a Mendelian fashion of segregation. Deviation from the expected segregation ratio was observed in a small number of the transgenic lines of P-I and P-II origins. However, this deviation was primarily observed in the P-III originated lines. Segregation analysis of the T 1 generation indicated that 1-3 copies of the cry1Ab gene were integrated into the genome of the majority of the transgenic lines originating from varieties P-I and P-II. Stunted and semi-fertile mutants were observed in some transgenic lines. These aberrations were either independent or closely linked to the introduced cry1Ab gene loci in diVerent transgenic lines. Reduction in GUS expression levels and loss of toxicity against YSB larvae were found in some transgenic lines. The transgenic T 3 and T 4 lines causing 100% mortality of third instar YSB larvae in the lab were completely protected in the Weld. Analysis of important yield components on nine selected transgenic lines indicated that stem length, panicle length, grain number per panicle, and seed setting rates were reduced in transgenic plants compared to those in non-transgenic parental rice lines. Number of panicles per cluster, however, was signiWcantly higher in transgenic plants. The numerical value of the average yield was in general greater in the controls than in all the transgenic lines, indicating some 'yield drag'. Since some selected lines were highly resistant to the YSB with good yielding potential, they oVer eVective potential for use in insect resistance management programs.

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Section: Discussionmentioning

confidence: 57%

Inheritance and field performance of transgenic Korean Bt rice lines resistant to rice yellow stem borer

Kim

et al. 2008

Euphytica

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“…The leaves were detached from the plants by their petioles. This was followed by the extraction of Cry1Ac protein at 72 h post-wounding period using a method described by Zaidi et al [23]. The extracted protein was used in western blot following SDS-PAGE.…”

Section: Protein Expression Analysismentioning

confidence: 99%

Expression of Cry1Ac in Transgenic Tobacco Plants Under the Control of a Wound-Inducible Promoter (AoPR1) Isolated from Asparagus officinalis to Control Heliothis virescens and Manduca sexta

et al. 2009

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Expression of cry1Ac gene from Bacillus thuringiensis (Bt) was evaluated under the control of a wound-inducible AoPR1 promoter from Asparagus officinalis in transgenic tobacco plants. The leaves of transgenic plants were mechanically wounded to evaluate the activity of the AoPR1 promoter in driving the expression of Cry1Ac protein at the wound site. Our results indicate that mechanical wounding of transgenic plants was effective in inducing the expression of Cry1Ac protein. As a result of this induction, the accumulated levels of Cry1Ac protein increased during 6-72 h post-wounding period. The leaves of transgenic tobacco plants were evaluated for resistance against Heliothis virescens and Manduca sexta in insect bioassays in two different ways. The detached tobacco leaves were either fed directly to the insect larvae or they were first mechanically wounded followed by a 72 h post-wounding feeding period. Complete protection of mechanically wounded leaves of transgenic plants was observed within 24 h of the bioassay. The leaves of transgenic plants fed directly (without pre-wounding) to the larvae achieved the same level of protection between 24 and 72 h of the bioassay.

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“…The high levels of Cry1Ca1 expression obtained in these transgenic plants are likely due to the codon usage which matches that of rice plants. Cheng et al (1998) [35] have described this as one of the strategies to increase the expression of cry1Ab and cry1Ac genes in transgenic rice plants up to a level of 3% [20,37,38]. A detailed study of the regions flanking the transgene insertions site in the plant genome and inclusion of more transgenic rice plants in the analysis, in our study, might be helpful in assessing any correlation between the copy number and the transgene expression levels.…”

Section: Discussionmentioning

confidence: 87%

“…The Cry1Ca1 protein was extracted from fresh leaves of transgenic rice plants using a method described by Zaidi et al (2005) [20]. Forty micrograms of protein extract was added to each well on SDS-PAGE for subsequent immunoblot analysis.…”

Section: Protein Expression Analysesmentioning

confidence: 99%

Transgenic Rice Plants Expressing a Modified cry1Ca1 Gene are Resistant to Spodoptera litura and Chilo suppressalis

et al. 2009

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Nucleotide sequence encoding the truncated insecticidal Cry1Ca1 protein from Bacillus thuringiensis was extensively modified based on the codon usage of rice genes. The overall G + C contents of the synthetic cry1Ca1 coding sequence were raised to 65% with an additional bias of enriching for G and C ending codons as preferred by monocots. The synthetic gene was introduced into the Chinese japonica variety, Xiushui 11, by Agrobacterium-mediated transformation. Transgenic rice plants harboring this gene were highly resistant to Chilo suppressalis and Spodoptera litura larvae as revealed by insect bioassays. High levels of Cry1Ca1 protein were obtained in the leaves of transgenic rice, which were effective in achieving 100% mortality of S. litura and C. suppressalis larvae. The levels of Cry1Ca1 expression in the leaves of these transgenic plants were up to 0.34% of the total soluble proteins. The larvae of C. suppressalis and S. litura could consume a maximum of 1.89 and 4.89 mm2 of transgenic leaf area whereas the consumption of nontransgenic leaves by these larvae was significantly higher; 58.33 and 61.22 mm2, respectively. Analysis of R1 transgenic plants indicated that the cry1Ca1 was inherited by the progeny plants and provided complete protection against C. suppressalis and S. litura larvae.

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The Bt gene cry2Aa2 driven by a tissue specific ST-LS1 promoter from potato effectively controls Heliothis virescens

Cited by 25 publications

References 32 publications

Inheritance and field performance of transgenic Korean Bt rice lines resistant to rice yellow stem borer

Inheritance and field performance of transgenic Korean Bt rice lines resistant to rice yellow stem borer

Expression of Cry1Ac in Transgenic Tobacco Plants Under the Control of a Wound-Inducible Promoter (AoPR1) Isolated from Asparagus officinalis to Control Heliothis virescens and Manduca sexta

Transgenic Rice Plants Expressing a Modified cry1Ca1 Gene are Resistant to Spodoptera litura and Chilo suppressalis

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