Transgenic Rice Plants Expressing a Modified cry1Ca1 Gene are Resistant to Spodoptera litura and Chilo suppressalis

Zaidi, Mohsin Abbas; Yè, Gōngyín; Yao, Hongwei; You, Taek H.; Loit, Evelin; Dean, Donald H.; Riazuddin, Sheikh; Altosaar, Illimar

doi:10.1007/s12033-009-9201-9

Cited by 30 publications

(11 citation statements)

References 38 publications

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“…Cao et al (1999) transformed broccoli with a modified Cry1C gene, and the transgenic plants showed high resistance to diamond back moth (Plutella xylostella). Recently insect resistant japonica rice was developed using the synthetic Cry1C gene which was found to be highly resistant to stemborers and leaf folders (Ye et al 2009a, b;Zaidi et al 2009). Therefore, it would appear that the Cry1C toxin can be a potential alternative to Cry1A toxin and that it can also be combined with other Cry1A genes.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium mediated transformation of indica rice (Oryza sativa L.) for insect resistance

Ignacimuthu¹,

Raveendar²

2010

Euphytica

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The rice leaffolder (RLF), Cnaphalocrocis medinalis is an important pest of rice that causes severe damage in many areas of the world. The plants were transformed with fully modified (plant codon optimized) synthetic Cry1C coding sequences as well as with the hpt and gus genes, coding for hygromycin phosphotransferase and b-glucuronidase, respectively. Cry1C sequences placed under the control of doubled 35S promoter plus the AMV leader sequence, and hpt and gus genes driven by cauliflower mosaic virus 35S promoter, were used in this study. Embryogenic calli after cocultivation with Agrobacterium were selected on the medium containing hygromycin B. A total of 67 hygromycinresistant plants were regenerated. PCR and Southern blot analyses of primary transformants revealed the stable integration of Cry1C coding sequences into the rice genome with predominant single copy integration. R 1 progeny plants disclosed a monogenic pattern (3:1) of transgene segregation as confirmed by molecular analyses. These transgenic lines were highly resistant to rice leaffolder (RLF), Cnaphalocrocis medinalis as revealed by insect bioassay.

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Section: Introductionmentioning

confidence: 99%

Agrobacterium mediated transformation of indica rice (Oryza sativa L.) for insect resistance

Ignacimuthu¹,

Raveendar²

2010

Euphytica

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“…However, we observed that level of expression of transgene and resistance was higher in the multiple copy line UR11 compared to other transgenic lines. Zaidi et al (2009) also reported that the level of Cry1C expression was higher in transgenic rice plant with three copies of cry1C gene when compared to single copy plants.…”

Section: Discussionmentioning

confidence: 96%

Transgenic rice plants expressing synthetic cry2AX1 gene exhibits resistance to rice leaffolder (Cnaphalocrosis medinalis)

et al. 2016

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Bacillus thuringiensis is a major source of insecticidal genes imparting insect resistance in transgenic plants. Level of expression of transgenes in transgenic plants is important to achieve desirable level of resistance against target insects. In order to achieve desirable level of expression, rice chloroplast transit peptide sequence was fused with synthetic cry2AX1 gene to target its protein in chloroplasts. Sixteen PCR positive lines of rice were generated by Agrobacterium mediated transformation using immature embryos. Southern blot hybridization analysis of T0 transgenic plants confirmed the integration of cry2AX1 gene in two to five locations of rice genome and ELISA demonstrated its expression. Concentration of Cry2AX1 in transgenic rice events ranged 5.0–120 ng/g of fresh leaf tissue. Insect bioassay of T0 transgenic rice plants against neonate larvae of rice leaffolder showed larval mortality ranging between 20 and 80 % in comparison to control plant. Stable inheritance and expression of cry2AX1 gene was demonstrated in T1 progenies through Southern and ELISA. In T1 progenies, the highest concentration of Cry2AX1 and mortality of rice leaffolder larvae were recorded as 150 ng/g of fresh leaf tissue and 80 %, respectively. The Cry2AX1 expression even at a very low concentration (120–150 ng/g) in transgenic rice plants was found effective against rice leaffolder larvae.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-015-0315-4) contains supplementary material, which is available to authorized users.

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“…Despite the development of at least 8 transgenic rice lines expressing toxin [3], [4], [5], [6], [7], [8], [9], no Bt rice lines have yet been approved for commercial release in China. However, one cultivar (Huahui 1) and its hybrid line (Shanyou 63), expressing Cry1Ab and Cry1Ac for resistance to lepidoperans, have been granted a limited trial in farmlands in Hubei Province for the five-year period 2009 through 2014 [10].…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation of Cry1Ab Protein from an Herbivore Reduces Anti-Oxidant Enzyme Activities in Two Spider Species

et al. 2014

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Cry proteins are expressed in rice lines for lepidopteran pest control. These proteins can be transferred from transgenic rice plants to non-target arthropods, including planthoppers and then to a predatory spider. Movement of Cry proteins through food webs may reduce fitness of non-target arthropods, although recent publications indicated no serious changes in non-target populations. Nonetheless, Cry protein intoxication influences gene expression in Cry-sensitive insects. We posed the hypothesis that Cry protein intoxication influences enzyme activities in spiders acting in tri-trophic food webs. Here we report on the outcomes of experiments designed to test our hypothesis with two spider species. We demonstrated that the movement of CryAb protein from Drosophila culture medium into fruit flies maintained on the CryAb containing medium and from the flies to the spiders Ummeliata insecticeps and Pardosa pseudoannulata. We also show that the activities of three key metabolic enzymes, acetylcholine esterase (AchE), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) were significantly influenced in the spiders after feeding on Cry1Ab-containing fruit flies. We infer from these data that Cry proteins originating in transgenic crops impacts non-target arthropods at the physiological and biochemical levels, which may be one mechanism of Cry protein-related reductions in fitness of non-target beneficial predators.

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Transgenic Rice Plants Expressing a Modified cry1Ca1 Gene are Resistant to Spodoptera litura and Chilo suppressalis

Cited by 30 publications

References 38 publications

Agrobacterium mediated transformation of indica rice (Oryza sativa L.) for insect resistance

Agrobacterium mediated transformation of indica rice (Oryza sativa L.) for insect resistance

Transgenic rice plants expressing synthetic cry2AX1 gene exhibits resistance to rice leaffolder (Cnaphalocrosis medinalis)

Bioaccumulation of Cry1Ab Protein from an Herbivore Reduces Anti-Oxidant Enzyme Activities in Two Spider Species

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