Agrobacterium mediated transformation of indica rice (Oryza sativa L.) for insect resistance

Ignacimuthu, S.; Raveendar, Sebastin

doi:10.1007/s10681-010-0308-7

Cited by 14 publications

(9 citation statements)

References 47 publications

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“…All these conditions of different parameters would help us in developing a congenial high throughput method of Agrobacterium mediated genetic transformation in maize. Similar studies were done in Rice (Ignacimuthu and Raveendar, 2011;Sahoo and Tuteja, 2012) and Yam (Nyaboga et al, 2014). This study will help in maize genetic engineering for agronomically demand traits into recalcitrant maize genotypes.…”

Section: Recovery Of Fertile Transgenic Plants Throughsupporting

confidence: 65%

Resourceful and High Efficiency Agrobacterium Mediated Transformation of Maize (Zea mays L.) Using Coleoptilar Nodal Explants

Sreenu¹,

Kumar²,

Reddy³

et al. 2016

Int. J. Curr. Res. Biosci. Plant Biol.

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A b s t r a c t A r t i c l e I n f oColeoptilar nodal explants, which are independent of season, were used to develop high efficiency transformation method in maize. Conditions which affect Agrobacterium-mediated genetic transformation have been standardized by using these explants on hygromycin selection regime. Hyper virulent Agrobacterium strain EHA105 containing gateway vector pMDC99 with Ascorbate -Glutathione pathway coding genes harboring with hygromycin phosphotransferase (hpt II) and bar as plant selectable marker genes was used. The survival frequency of calli at three different stages viz., at the end of I sub-culture on normal medium and II selection as well as on regeneration media supplemented with hygromycin were taken into consideration for the assessment of optimal conditions after checking with PCR. Of the different parameters used with different conditions, one day pre-conditioning of explants, 0.8 optical density of bacterial culture in plain MS liquid infection medium having 5.8 pH along with 200 µM acetosyrengone, 15 minutes infection time for the explants after treating with 0.1% Macerozyme, application of vacuum infiltration for 10 minutes, three days of co-cultivation period were found to be optimal for getting high frequency of transformation through Agrobacterium-mediated genetic transformation in maize. Transformation frequency of 3.25% was obtained on average with these optimal conditions, for which the explants were co-cultivated with these conditions and inoculated on callus induction medium having hygromycin at 10 mg l -1 for selection. The selected calli were transferred onto regeneration medium supplemented with BAP and Kinetin, at a concentration of 1 mg l -1 each for plantlet development. Putative transformants were screened by using hpt II gene specific primers and confirmed after performing southern analysis.

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Section: Recovery Of Fertile Transgenic Plants Throughsupporting

confidence: 65%

Resourceful and High Efficiency Agrobacterium Mediated Transformation of Maize (Zea mays L.) Using Coleoptilar Nodal Explants

Sreenu¹,

Kumar²,

Reddy³

et al. 2016

Int. J. Curr. Res. Biosci. Plant Biol.

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“…Among the factors that affect frequency of callus induction and plant regeneration in indica rice, genotype, physiological status of the seed scutellum, type and dose of the plant growth regulators among others are very important (Ge et al, 2006). Although there are several reports of successful regeneration of transgenic indica rice from Agrobacterium-mediated transformation of embryogenic calli (Ignacimuthu andArockiasamy 2006, Mohanty et al,2002), many indica varieties are still recalcitrant to cultural manipulations due to poor callus induction, proliferation and regeneration abilities (Islam et al,2004, Khaleda and Al-Forkan 2006, Zuraida et al, 2011. Difficulties still exist in establishing a widely used culture system for indica rice applicable to wide range of cultivars.…”

Section: Introductionmentioning

confidence: 99%

Establishment of an Efficient Regeneration System Amenable to Agrobacterium Mediated Transformation of Two Elite Indica Rice Varieties of North East India

Dey

Panda

Sahoo

2015

Int J Appl Sci Biotechnol

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An efficient plant regeneration system from embryogenic callus of two elite indica rice (Oryza sativa spp. indica) varieties of Northeast India, Ketokijoha and Monoharsali is established. The effect of auxin, 2,4-dicholorophenoxy acetic acid (2,4-D) on callus induction was optimized. Friable, nodular and creamish-white embryogenic calli were induced from mature seeds on NB medium supplemented with 2.5 mg/l 2,4-D. Plants were regenerated from 40-50 days old embryogenic callus on NB medium containing 0.5 mg/l BAP (6-benzylaminopurine) and 0.25 mg/l ABA (abscisic acid). Regenerated plants with multiple tillers were rooted on half strength MS medium and rooted plants were acclimatized with 94% survival rate. Higher frequency of callus induction as well as plant regeneration was recorded in Ketokijoha as compared to Monoharsali. The calli of both the varieties were found amenable to Agrobacterium-mediated transformation as evident from strong GUS (β-glucuronidase) expression. The results may find wide application for genetic improvement for valuable traits these elite indica rice varieties of Northeast India. Int J Appl Sci Biotechnol, Vol 3(4): 680-686

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“…Most plant regeneration systems adapted to Agrobactrerium -mediated genetic transformation in indica rice varieties (Rashid et al 1996; Aldemita and Hodges 1996; Nayak et al 1997; Zhang et al 1997; Khanna and Raina 1999, 2002; Mohanty et al. 2002; Supertana et al 2005; Ignacimuthu and Arockiasamy 2006) involve regeneration of plants from transformed embryogenic calli, anther calli (Jiang et al 2004), and protoplasts. Success in transformation of indica rice using such regeneration systems depends on the factors that favor the formation of friable and high quality callus in a shorter time competent for shoot regeneration.…”

Section: Introductionmentioning

confidence: 99%

Development of a genotype independent and transformation amenable regeneration system from shoot apex in rice (Oryza sativa spp. indica) using TDZ

et al. 2012

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Agrobacterium-mediated transformation of indica rice has been established in only a limited number of cultivars because the regeneration of plants from transformed embryogenic calli is highly cultivar-specific. Establishment of a highly efficient plant regeneration system from shoot apex explants applicable to many cultivars of indica rice will accelerate the application of transformation technology in breeding programs and functional genomics study. We established an efficient shoot multiplication and plant regeneration system from shoot apices of indica rice using thidiazuron (TDZ) as a plant growth regulator. Shoot apices cultured on MS basal medium devoid of plant growth regulators formed solitary shoots in 90% of cultures. Addition of TDZ or benzylaminopurine to regeneration medium significantly influenced formation of multiple shoots directly from shoot apex explants without an intervening callus stage. Best shoot proliferation response (10.3 shoots per explant) was recorded when shoot apices were cultured on media supplemented with 4 mg/l TDZ. No synergistic effect on shoot proliferation was observed when indole-3-acetic acid and indole-3-butyric acid were supplemented to media containing 4 mg/l TDZ. The regeneration system was efficient in evoking multiple shoot proliferation in eight different cultivars of indica rice. Shoots were rooted in MS basal medium and plantlets were acclimatized with 100% survival rate. The shoot apex explants of all the eight cultivars of indica rice were found competent to Agrobacterium-mediated transformation while explants from IR-64 showed highest transient GUS expression. This variety-independent transformation amenable regeneration system from shoot apices may widely be applicable for genetic transformation of indica varieties.

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Agrobacterium mediated transformation of indica rice (Oryza sativa L.) for insect resistance

Cited by 14 publications

References 47 publications

Resourceful and High Efficiency Agrobacterium Mediated Transformation of Maize (Zea mays L.) Using Coleoptilar Nodal Explants

Resourceful and High Efficiency Agrobacterium Mediated Transformation of Maize (Zea mays L.) Using Coleoptilar Nodal Explants

Establishment of an Efficient Regeneration System Amenable to Agrobacterium Mediated Transformation of Two Elite Indica Rice Varieties of North East India

Development of a genotype independent and transformation amenable regeneration system from shoot apex in rice (Oryza sativa spp. indica) using TDZ

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