The bridge between TLC and HPLC: overpressured layer chromatography (OPLC)

Nyiredy, Szabolcs

doi:10.1016/s0165-9936(00)00047-9

Cited by 40 publications

(9 citation statements)

References 7 publications

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“…Several forced-flow TLC methods have been introduced in recent years for connection with ESI-MS, and a compact and simple instrument for overpressure layer chromatography (OPLC) has been commercially available for several years (Nyiredy, 2001). In OPLC, the plate is covered with an inert membrane sheet under external pressure, and the mobile phase is pumped through the sealed layer of the stationary phase, such as À molecular ions at m/z 1,545.1 and m/z at 1,573.1 correspond to GM1 (d18:1, C16:0) and GM1 (d18:1, C18:0), respectively (for details see Luftmann, 2004).…”

Section: Ms Coupled To On-line Overpressured Layer Chromatographymentioning

confidence: 99%

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Advances on the compositional analysis of glycosphingolipids combining thin-layer chromatography with mass spectrometry

Müthing

Distler

2009

Mass Spectrom. Rev.

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Glycosphingolipids (GSLs), composed of a hydrophilic carbohydrate chain and a lipophilic ceramide anchor, play pivotal roles in countless biological processes, including infectious diseases and the development of cancer. Knowledge of the number and sequence of monosaccharides and their anomeric configuration and linkage type, which make up the principal items of the glyco code of biologically active carbohydrate chains, is essential for exploring the function of GSLs. As part of the investigation of the vertebrate glycome, GSL analysis is undergoing rapid expansion owing to the application of novel biochemical and biophysical technologies. Mass spectrometry (MS) takes part in the network of collaborations to further unravel structural and functional aspects within the fascinating world of GSLs with the ultimate aim to better define their role in human health and disease. However, a single-method analytical MS technique without supporting tools is limited yielding only partial structural information. Because of its superior resolving power, robustness, and easy handling, high-performance thin-layer chromatography (TLC) is widely used as an invaluable tool in GSL analysis. The intention of this review is to give an insight into current advances obtained by coupling supplementary techniques such as TLC and mass spectrometry. A retrospective view of the development of this concept and the recent improvements by merging (1) TLC separation of GSLs, (2) their detection with oligosaccharide-specific proteins, and (3) in situ MS analysis of protein-detected GSLs directly on the TLC plate, are provided. The procedure works on a nanogram scale and was successfully applied to the identification of cancer-associated GSLs in several types of human tumors. The combination of these two supplementary techniques opens new doors by delivering specific structural information of trace quantities of GSLs with only limited investment in sample preparation.

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Section: Ms Coupled To On-line Overpressured Layer Chromatographymentioning

confidence: 99%

“…The plate equates to a planar column and has some advantages compared to conventional TLC (Nyiredy, 2001). Linking TLC with ESI-MS has been particularly difficult, mainly because of incompatibility between the dynamic liquid-phase sample introduction of ESI-MS and the static nature of TLC.…”

Section: Gsl Analysis Matching Tlc With Msmentioning

confidence: 99%

Advances on the compositional analysis of glycosphingolipids combining thin-layer chromatography with mass spectrometry

Müthing

Distler

2009

Mass Spectrom. Rev.

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“…14). HPTLC exhibits a two times higher efficiency than TLC over a short distance of migration (up to about 10 cm) (Nyiredy 2001). Except for OPLC (described in Sect.…”

Section: Thin-layer Chromatography and High-performance Thin-layer Chmentioning

confidence: 99%

“…It requires specific equipment (Fig. 9) but the separation is faster and the resolution is highly improved (Nyiredy 2001). OPLC can be used either for analytical or semi-preparative purposes (Bryson and Papillard 2004;Tyihak et al 2012).…”

Section: Planar Chromatographymentioning

confidence: 99%

The Modern Art of Identification of Natural Substances in Whole Plants

Chaimbault

2014

Recent Advances in Redox Active Plant and Microbial Products

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“…In recent years we have reported the development and successful analytical and preparative application of new forced-flow planar chromatographic techniques like rotation planar chromatography [3] and overpressured layer chromatography [4]. The influence of operating parameters using medium-pressure liquid chromatography (MPLC) [5 6] and the prediction of the efficiency of MPLC separations calculated from results of overpressured layer chromatographic data were also reported [4].…”

Section: Introductionmentioning

confidence: 99%

Medium-pressure solid-liquid extraction: A new preparative method based on the principle of counter-current

Nyiredy¹,

Botz

2003

Chromatographia

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SummaryA novel variant of continuous relative counter-current extraction, called medium-pressure solid-liquid extraction (MPSLE) is introduced as a new preparative separation method for laboratory purposes. This method is a special case of exhaustive extraction.On using MPSLE, the extraction column, i.e. a medium pressure liquid chromatographic (MPLC) column is filled with the fine-powdered solid phase to be extracted, and extraction solvent(s) is/are forced through the stationary bed by means of a pump. This method constitutes the relative counter-current extraction, and results in exhaustive and rapid extraction. By MPSLE, efficiency is achieved not just because the extraction solvent(s) is/are forced through the staying material to be extracted, but also clue to the overpressure in the extraction column and the suitable changes of the dissolution and diffusion periods of the process.The method can be used for the rapid extraction of various substance classes occurring as complex solid matrices. Experiments carried out with MPLC columns show that the exhaustive extraction of finely powdered plant material of 100-3000g can be extracted and concentrated with an automated equipment within a few hours. Further advantages of the environment-friendly process performed in closed system are full automation and operation with low qua ntil',/of extracta nt.

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The bridge between TLC and HPLC: overpressured layer chromatography (OPLC)

Cited by 40 publications

References 7 publications

Advances on the compositional analysis of glycosphingolipids combining thin-layer chromatography with mass spectrometry

Advances on the compositional analysis of glycosphingolipids combining thin-layer chromatography with mass spectrometry

The Modern Art of Identification of Natural Substances in Whole Plants

Medium-pressure solid-liquid extraction: A new preparative method based on the principle of counter-current

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