The Modern Art of Identification of Natural Substances in Whole Plants

Chaimbault, Patrick

doi:10.1007/978-94-017-8953-0_3

Cited by 4 publications

(2 citation statements)

References 273 publications

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“…FC constitutes on of the simplest methodologies of maximizing the quantities and purity of natural active isolates, for their supplementary structural interpretation through NMR spectroscopy. Regardless its lower resolution compared to other techniques, FC has the benefits of being simple and inexpensive [ 106 ].…”

Section: Hyphenated Chromatographic Techniquesmentioning

confidence: 99%

Qualitative and Quantitative Analysis of Polyphenols in Lamiaceae Plants—A Review

Tzima

Brunton

Dilip

2018

Plants

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Lamiaceae species are promising potential sources of natural antioxidants, owing to their high polyphenol content. In addition, increasing scientific and epidemiological evidence have associated consumption of foods rich in polyphenols with health benefits such as decreased risk of cardiovascular diseases mediated through anti-inflammatory effects. The complex and diverse nature of polyphenols and the huge variation in their levels in commonly consumed herbs make their analysis challenging. Innovative robust analytical tools are constantly developing to meet these challenges. In this review, we present advances in the state of the art for the identification and quantification of polyphenols in Lamiaceae species. Novel chromatographic techniques that have been employed in the past decades are discussed, ranging from ultra-high-pressure liquid chromatography to hyphenated spectroscopic methods, whereas performance characteristics such as selectivity and specificity are also summarized.

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Section: Hyphenated Chromatographic Techniquesmentioning

confidence: 99%

Qualitative and Quantitative Analysis of Polyphenols in Lamiaceae Plants—A Review

Tzima

Brunton

Dilip

2018

Plants

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“…In recent years, the use of liquid chromatography (LC) coupled to mass spectrometry (MS) has become an alternative approach for protein quantitation in a wide range of sectors, including biotechnology, environmental monitoring, food processing, pharmaceuticals, agrochemicals, and cosmetics. , The pairing of LC to tandem MS (LC–MS/MS) combines the physical separation capabilities of LC with the specific mass identification capabilities of MS. The LC–MS/MS technology also supports multiplexed and high through put protein analysis.…”

Section: Introductionmentioning

confidence: 99%

Immunoassays and Mass Spectrometry for Determination of Protein Concentrations in Genetically Modified Crops

Wang,

Bednarcik,

Ament

et al. 2024

J. Agric. Food Chem.

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Quantifying protein levels in genetically modified (GM) crops is crucial in every phase of development, deregulation, and seed production. Immunoassays, particularly enzyme-linked immunosorbent assay, have been the primary protein quantitation techniques for decades within the industry due to their efficiency, adaptability, and credibility. Newer immunoassay technologies like Meso Scale Discovery and Luminex offer enhanced sensitivity and multiplexing capabilities. While mass spectrometry (MS) has been widely used for small molecules and protein detection in the pharmaceutical and agricultural industries (e.g., biomarkers, endogenous allergens), its use in quantifying protein levels in GM crops has been limited. However, as trait portfolios for GM crop have expanded, MS has been increasingly adopted due to its comparable sensitivity, increased specificity, and multiplexing capabilities. This review contrasts the benefits and limitations of immunoassays and MS technologies for protein measurement in GM crops, considering factors such as cost, convenience, and specific analytical needs. Ultimately, both techniques are suitable for assessing protein concentrations in GM crops, with MS offering complementary capabilities to immunoassays. This comparison aims to provide insights into selecting between these techniques based on the user's end point needs.

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Phytochemicals with Anticancer Potential: Methods of Extraction, Basic Structure, and Chemotherapeutic Action

Nizami

Sayyed

2018

Anticancer Plants: Properties and Application

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The Modern Art of Identification of Natural Substances in Whole Plants

Cited by 4 publications

References 273 publications

Qualitative and Quantitative Analysis of Polyphenols in Lamiaceae Plants—A Review

Qualitative and Quantitative Analysis of Polyphenols in Lamiaceae Plants—A Review

Immunoassays and Mass Spectrometry for Determination of Protein Concentrations in Genetically Modified Crops

Phytochemicals with Anticancer Potential: Methods of Extraction, Basic Structure, and Chemotherapeutic Action

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