The bovine B and C blood group systems are not likely to be the orthologues of human RH: an interesting twist in the comparative map

Abstract

We tested the hypothesis that either the bovine B or C blood group system is the orthologue of human RH. A comparative linkage mapping strategy was applied, using blood typing and restriction fragment length polymorphism (RFLP) analysis of four loci linked to RH on HSA1; PGD, FGR, ALPL and FUCA1. Four sires with a total of 255 half-sib offspring were used for the linkage analysis. Strong support for linkage between ALPL, FUCA1 and FGR was obtained for all sire families (lod scores > 11 for all pairwise comparisons). This new linkage group was assigned to bovine synteny group U17 based on previous somatic cell mapping of the FGR locus. The most favoured order is ALPL-FUCA1-FGR (2.18:1), with ALPL and FGR 5.4 CM and 6.3 CM, respectively, from FUCA1. The B and C blood group systems and PGD were genetically independent of each other and all other markers, indicating that neither B nor C is likely to be the bovine orthologue of human RH. However, given available comparative mapping data, there is some chance that the bovine orthologue of RH is on bovine synteny group U6. Although gene order appears to be conserved with humans, the differences in recombination rates between these three loci in cattle, humans and mice strongly suggest that it is not possible to use human map distances to predict map distances in cattle, making it imperative that bovine gene mappers continue to emphasize adding type I markers to the bovine linkage map.

Molecular Cloning, Genetic Mapping, and Developmental Expression of Bovine POU5F11

“…Nine bulls that sire paternal half-sib families collectively known as the Illinois Reference and Resource Families (IRRF; [22]) were screened for a polymorphism in bPOU5F1 by single-strand conformational polymorphism (SSCP) analysis as described [23]. Polymerase chain reaction (PCR) products for SSCP analysis were generated using oligonucleotide primers OCT27 (5Ј-GGAAATTGGGAACACAATGGG-3Ј, coding strand) and OCT28 (5Ј-AACAGCAACCTTCGT-TTCGG-3Ј, noncoding strand), which are both located downstream of the predicted stop codon of bPOU5F1 (see Fig.…”

Genetic mapping of five human chromosome 4 orthologues to bovine chromosomes 6 and 17

“…A DNA panel of nine individuals from different cattle breeds was used for the identification of polymorphisms using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and single-strand conformational polymorphism (SSCP) methods. The nine paternal half-sib families of the Illinois Reference/ Resource Families (IRRF; Lewin et al 1994;Ma et al 1996) were used for linkage studies.…”

Report of the First Workshop on the Genetic Map of Bovine Chromosome 23

“…Statistical criteria for the construction of the framework map were as described by Keats et al (1991). Briefly, framework loci were defined as a group of loci Bishop et al 1994) and Texas A&M University Angleton Families (TAMU; J. F. Taylor & S. K. Davis, unpublished observations] were analysed using a sex-specific analysis, whereas data from the Illinois Reference/Resource Families (IRRF; Lewin et al 1994;Ma et al 1996) and the Swedish Agricultural University Pedigrees (UPP; Anderson et al 1988) contained segregation information from males only. The C H R O M P l C option of C R I M A P was used to identify and eliminate animals having unlikely multiple recombination events, where unlikely recombination events were defined as those occurring more frequently than would be expected based on the product of the pairwise recombination frequencies (€))of the loci involved.…”