Molecular Cloning, Genetic Mapping, and Developmental Expression of Bovine POU5F11

Eijk, Michiel J. T. van; Rooijen, Marga A. van; Modina, S.; Scesi, L. Baraldi; Folkers, Gert E.; Tol, Helena T A van; Bevers, M.M.; Fisher, Simon; Lewin, Harris A.; Rakacolli, D.; Galli, Cesare; Vaureix, Christiane de; Trounson, Alan; Mummery, Christine L.; Gandolfi, F.

doi:10.1095/biolreprod60.5.1093

Cited by 166 publications

(126 citation statements)

References 42 publications

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“…However, lower expression levels in the in vivo blastocysts may be a consequence of higher HDAC1 activity and differentiation [69]. In murine and human expanded blastocysts, expression of OCT4 was limited to the ICM [20,22,70,71] whereas in bovine and porcine blastocysts, OCT4 was expressed in both the trophectoderm and ICM [72,73]. Localization of OCT4 was diffused in the early stages of rabbit preimplantation embryos; while nuclear localization was evident in the blastocyst.…”

Section: Discussionmentioning

confidence: 99%

Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro

Gibence

Brahmasani

Mahesh

et al. 2014

J Assist Reprod Genet

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Purpose The levels and timing of expression of genes like BCLXL, HDAC1 and pluripotency marker genes namely, OCT4, SOX2, NANOG and KLF4 are known to influence preimplantation embryo development. Despite this information, precise understanding of their influence during preimplantation embryo development is lacking. The present study attempts to compare the expression of these genes in the in vivo and in vitro developed preimplantation embryos. Methods The in vivo and in vitro developed rabbit embryos collected at distinct developmental stages namely, pronuclear, 2 cell, 4 cell, 8 cell, 16 cell, Morula and blastocyst were compared at the transcriptional and translational levels using Real Time PCR and immunocytochemical studies respectively. Results The study establishes the altered levels of candidate genes at the transcriptional level and translational level with reference to the zygotic genome activation (ZGA) phase of embryo development in the in vivo and in vitro developed embryos. The expression of OCT4, KLF4, NANOG and SOX2 genes were higher in the in vitro developed embryos whereas and HDAC1 was lower. BCLXL expression had its peak at ZGA in in vivo developed embryos. Protein expression of all the candidate genes was observed in the embryos. BCLXL, KLF4 and NANOG exhibited diffused localisation whereas HDAC1, OCT4, and SOX2 exhibited nuclear localisation. Conclusions This study leads to conclude that BCLXL peak expression at the ZGA phase may be a requirement for embryo development. Further expression of all the candidate genes was influenced by ZGA phase of development at the transcript level, but not at the protein level.

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Section: Discussionmentioning

confidence: 99%

Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro

Gibence

Brahmasani

Mahesh

et al. 2014

J Assist Reprod Genet

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“…We did not observe H3K27 methylation differences between ICM and TE cells of bovine fertilized embryos. It is worth noticing that POU5F1 protein is detected in both ICM and TE cells of bovine blastocyst (van Eijk et al 1999, Kirchhof et al 2000, and therefore is possible that the differences in H3K27me3 between pluripotent and trophoblast cells are established at later stages of bovine development.…”

Section: H3k27me3 and Pcg Gene Expression In Bovine Embryosmentioning

confidence: 99%

Polycomb gene expression and histone H3 lysine 27 trimethylation changes during bovine preimplantation development

Ross¹,

Ragina²,

Rodríguez³

et al. 2008

Reproduction

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Trimethylation of histone H3 at lysine 27 (H3K27me3) is established by polycomb group genes and is associated with stable and heritable gene silencing. The aim of this study was to characterize the expression of polycomb genes and the dynamics of H3K27me3 during bovine oocyte maturation and preimplantation development. Oocytes and in vitro-produced embryos were collected at different stages of development. Polycomb gene expression was analyzed by real-time quantitative RT-PCR and immunofluorescence. Global H3K27me3 levels were determined by semiquantitative immunofluorescence. Transcripts for EZH2, EED, and SUZ12 were detected at all stages analyzed, with EZH2 levels being the highest of the three at early stages of development. By the time the embryo reached the blastocyst stage, the level of PcG gene mRNA levels significantly increased. Immunofluorescence staining indicated nuclear expression of EZH2 at all stages while nuclear localized EED and SUZ12 were only evident at the morula and blastocyst stages. Semiquantitative analysis of H3K27me3 levels showed that nuclear fluorescence intensity was the highest in immature oocytes, which steadily decreased after fertilization to reach a nadir at the eight-cell stage, and then increased at the blastocyst stage. These results suggest that the absence of polycomb repressive complex 2 proteins localized to the nucleus of early embryos could be responsible for the gradual decrease in H3K27me3 during early preimplantation development. Reproduction (2008) 136 777-785

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“…Protein expression patterns are similar to the expression patterns of POU5F1 mRNA during preimplantation development. By contrast, in cattle and swine embryos, the pattern of POU5F1 expression in the blastocyst is reported to differ, it is found in both the TE and ICM (van Eijk et al 1999, Kirchhof et al 2000. POU5F1 mRNA expression in bovine blastocysts is unclear; it has been reported to be only in the ICM (Kurosaka et al 2004), or in both the ICM and TE (Degrelle et al 2005).…”

Section: Introductionmentioning

confidence: 95%

“…In contrast, it is thought that POU5F1 protein is not suppressed in the TE of bovine embryos in vivo or in vitro. This understanding is based largely on the findings of van Eijk et al (1999) and Kirchhof et al (2000). There are some areas of confusion in these reports; however, van Eijk et al (1999) found POU5F1 protein immunostaining in ICM and TE of IVP and oviductal embryos, but no POU5F1 protein immunostaining at all in uterine embryos examined at days 14 and 16.…”

Section: Pou5f1 In Equine Embryosmentioning

confidence: 99%

The uterine environment modulates trophectodermal POU5F1 levels in equine blastocysts

Choi¹,

Harding²,

Hartman³

et al. 2009

Reproduction

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The reported patterns of trophectodermal expression of POU5F1 protein in blastocysts vary among species, and are possibly related to the differences in placental growth and function. This study investigated the pattern of embryonic POU5F1 expression in the horse, a species with delayed placental formation. Immature equine oocytes expressed POU5F1 protein in the cytoplasm and nucleus. Staining for POU5F1 protein in in vitro-produced (IVP) embryos decreased to day 5 of culture, then the nuclear staining increased to day 7. IVP day-7 to -11 blastocysts showed POU5F1 staining in nuclei throughout the blastocysts. In contrast, in vivo-produced day-7 to -10 blastocysts showed greatly reduced trophoectodermal POU5F1 protein expression. To determine whether the uterine environment modulates POU5F1 expression, IVP blastocysts were transferred to the uteri of mares, then recovered 2-3 days later (IVP-ET embryos). These embryos showed similar POU5F1 expression as the in vivo-produced embryos. Levels of POU5F1, SOX2, and NANOG mRNA in IVP-ET blastocysts were significantly higher in the inner cell mass than in trophectoderm (TE) cells. These data suggest that the differentiation of equine TE, as indicated by loss of POU5F1 expression, is impaired during in vitro culture, but proceeds normally when the embryos are exposed to the uterine environment. Previously reported differences in trophectodermal expression of POU5F1 among species may thus be in part artifactual, i.e. related to in vitro culture. Failure for correction of such changes by the uterine environment is a potential factor in the placental abnormalities seen after transfer of cultured embryos in some species.

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Molecular Cloning, Genetic Mapping, and Developmental Expression of Bovine POU5F11

Cited by 166 publications

References 42 publications

Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro

Candidate gene expression patterns in rabbit preimplantation embryos developed in vivo and in vitro

Polycomb gene expression and histone H3 lysine 27 trimethylation changes during bovine preimplantation development

The uterine environment modulates trophectodermal POU5F1 levels in equine blastocysts

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