2015
DOI: 10.1016/j.jplph.2014.09.013
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The biphasic interphase-mitotic polarity of cell nuclei induced under DNA replication stress seems to be correlated with Pin2 localization in root meristems of Allium cepa

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Cited by 6 publications
(9 citation statements)
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“…1 B). In accordance with our earlier data (Żabka et al 2012 ; 2015a ), cell size seems to constitute one the major parameters determining either one or the other of the two abnormal types of nuclear division. The IM cell nuclei (also termed as “octopus-like” for their chromosomal arms sticking out of the interphase nuclear bodies) are restricted merely to a group to extremely long cells with major to minor axis ratios exceeding a factor of 3.0, whereas the PCC-like phenotype is confined to a group of M-phase cells approaching the maximum length to width ratio less than 2.5.…”
Section: Resultssupporting
confidence: 92%
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“…1 B). In accordance with our earlier data (Żabka et al 2012 ; 2015a ), cell size seems to constitute one the major parameters determining either one or the other of the two abnormal types of nuclear division. The IM cell nuclei (also termed as “octopus-like” for their chromosomal arms sticking out of the interphase nuclear bodies) are restricted merely to a group to extremely long cells with major to minor axis ratios exceeding a factor of 3.0, whereas the PCC-like phenotype is confined to a group of M-phase cells approaching the maximum length to width ratio less than 2.5.…”
Section: Resultssupporting
confidence: 92%
“…Evidence from our previous studies (Żabka et al 2010 , 2012 , 2013 , 2015a , b ) indicated that continuous treatment of onion RAMs with a low concentration of HU may disrupt crucial functions of the checkpoint response system, leading cells to abnormal mitotic progression manifested by premature chromosome condensation (PCC phenotype) and by axially elongated cells with half interphase/half mitotic structures (IM phenotype having both dispersed and condensed domains of chromatin). Our current work shows that prolonged incubations of 4-day-old primary roots of A. cepa with many chemical agents (including those commonly used to inhibit DNA replication) can induce interphase–mitotic organization of nuclear chromatin structures.…”
Section: Discussionmentioning
confidence: 99%
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“…Prior to our study on generative and non-generative antheridial cell systems, the specificity of polyclonal antibody raised against synthetic peptides derived from AtPIN2 sequences (encoded by the locus At5g57090) was tested by immunoprecipitation (IP) using total protein extracts from both apical parts of Chara thalli and root tips excised from Arabidopsis thaliana seedlings (as previously described; Żabka et al 2015 ). For each of these samples, IP followed by Western blot assay revealed two bands at about 49 and about 60 kDa (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Immunoprecipitation and protein blot assays for PIN2-LPs extracted from apical parts of C. vulgaris carrying whorls with young antheridia and (as a control) for PIN2 proteins extracted from root tips of A. thaliana (0.5–1 mm in length) were performed according to methods described earlier (Żabka et al 2015 ). Briefly, excised plant materials were lysed using a P-PER Plant Protein Extraction Kit (Pierce, Rockford, IL, USA) containing Protease Inhibitor Cocktail (P-9599; Sigma-Aldrich) and the extracts were cleared afterwards by centrifugation.…”
Section: Methodsmentioning
confidence: 99%