PIN2-like proteins may contribute to the regulation of morphogenetic processes during spermatogenesis in Chara vulgaris

Żabka, Aneta; Polit, Justyna Teresa; Winnicki, Konrad; Paciorek, Patrycja; Juszczak, Jolanta; Nowak, Mateusz; Maszewski, Janusz

doi:10.1007/s00299-016-1979-x

Cited by 21 publications

(11 citation statements)

References 60 publications

(85 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In land plants, polar auxin transport (PAT) is a key factor in the spatiotemporal control of development by asymmetric subcellular auxin distribution and is mediated by plasma membrane (PM) localized PIN proteins (Swarup and Bennett, 2014). The presence of PAT (Boot et al, 2012) and the polarized expression of PINs in charophytes (Żabka et al, 2016) suggest that PIN-mediated PAT may have originated in charophytes, although their relocalization to the plasma membrane from an ancestral form in the endoplasmic reticulum may have been key to the development of early land plants (Viaene et al, 2012). In conclusion, while it appears that the canonical auxin biosynthetic and signaling pathways were derived from the assembly and neofunctionalization of molecular interactions that existed in the ancestral land plant, the P. margaritaceum genome sequence has revealed additional core auxin signaling components that likely emerged in the Zygnematophyceae.…”

Section: Resultsmentioning

confidence: 99%

The Genome of the Charophyte AlgaPenium margaritaceumBears Footprints of the Evolutionary Origins of Land Plants

Jiao

Sørensen

Sun

et al. 2019

Preprint

View full text Add to dashboard Cite

30The colonization of land by plants was a pivotal event in the history of the biosphere, and yet the 31 underlying evolutionary features and innovations of the first land plant ancestors are not well 32 understood. Here we present the genome sequence of the unicellular alga Penium margaritaceum, 33 a member of the Zygnematophyceae, the sister lineage to land plants. The P. margaritaceum 34 genome has a high proportion of repeat sequences, which are associated with massive segmental 35 gene duplications, likely facilitating neofunctionalization. Compared with earlier diverging plant 36 lineages, P. margaritaceum has uniquely expanded repertoires of gene families, signaling 37 networks and adaptive responses, supporting its phylogenetic placement and highlighting the 38 evolutionary trajectory towards terrestrialization. These encompass a broad range of physiological 39 processes and cellular structures, such as large families of extracellular polymer biosynthetic and 40 modifying enzymes involved in cell wall assembly and remodeling. Transcriptome profiling of 41 cells exposed to conditions that are common in terrestrial habitats, namely high light and 42 desiccation, further elucidated key adaptations to the semi-aquatic ecosystems that are home to the 43 Zygnematophyceae. Such habitats, in which a simpler body plan would be advantageous, likely 44 provided the evolutionary crucible in which selective pressures shaped the transition to land. 45 Earlier diverging charophyte lineages that are characterized by more complex land plant-like 46 anatomies have either remained exclusively aquatic, or developed alternative life styles that allow 47 109 of 116.1 kb. The nuclear assembly captured most of the k-mers in the Illumina reads and low 110 frequency k-mers representing sequencing errors were absent ( Supplementary Fig. 1B). In addition, 111 the mapping rates of genomic and RNA-Seq reads against the nuclear assembly were 97.5% and 112 5 96.8%, respectively (Supplementary Table 2). The single nucleotide polymorphism (SNP) 113 frequency distribution on the 100 longest scaffolds was consistent with a haploid genome 114 ( Supplementary Fig. 1C). The mitochondrial and chloroplast genomes were also fully assembled, 115 and comprised 95,332 and 145,411 nucleotides, respectively ( Supplementary Fig. 2). 116The assembly contains a large proportion (80.6%) of repeat sequences (Supplementary 117 Table 3), particularly long terminal repeat (LTR) retrotransposons and simple repeats ( Fig. 2A). 118Unlike land plants and C. braunii, in which gypsy is the predominant LTR family, the P. 119 margaritaceum genome has a large proportion of copia retrotransposons, which are rare in other 120 green algae and absent from C. braunii (Nishiyama et al. 2018). An estimation of divergence time 121 indicated that the copia expansion in the P. margaritaceum genome was relatively recent, around 122 2.1 Mya (Fig. 2B). Retrotransposons carrying tyrosine recombinases, such as the DIRS and Ngaro 123 families, which are found in some chlorophyte...

show abstract

Section: Resultsmentioning

confidence: 99%

The Genome of the Charophyte AlgaPenium margaritaceumBears Footprints of the Evolutionary Origins of Land Plants

Jiao

Sørensen

Sun

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Furthermore, PIN-family genes have been identified in the charophyte algae Spirogyra pratenis (De Smet et al, 2011), Klebsormidium flaccidum UTEX321 (Viaene et al, 2013;see below) and Klebsormidium nitens NIES-2285 (Hori et al, 2014;see below) by transcriptome and genome analyses, but their functions in auxin transport remain unclear. The existence of polar auxin transport is supported in several Chara species (Dibb-Fuller andMorris, 1992: Klambt et al, 1992;Boot et al, 2012;Zhang and van Duijn, 2014;_ Zabka et al, 2016). Therefore, unraveling the auxin system in charophyta-the polyphyletic group that diverged during the evolution of land plants from a green algae ancestor-will be helpful for clarifying the origin and evolution of auxin function and signaling.…”

mentioning

confidence: 99%

Primitive Auxin Response without TIR1 and Aux/IAA in the Charophyte Alga Klebsormidium nitens

et al. 2017

View full text Add to dashboard Cite

The phytohormone auxin regulates many aspects of growth and development in land plants, but the origin and evolution of auxin signaling and response mechanisms remain largely unknown. Indeed, it remains to be investigated whether auxin-related pathways diverged before the emergence of land plants. To address this knowledge deficit, we analyzed auxin responses in the charophyte alga Klebsormidium nitens NIES-2285, whose ancestor diverged from a green algal ancestor during the evolution of land plants. This strain is the same as Klebsormidium flaccidum NIES-2285, for which the draft genome was sequenced in 2014, and was taxonomically reclassified as K. nitens. This genome sequence revealed genes involved in auxin responses. Furthermore, the auxin indole-3-acetic acid (IAA) was detected in cultures of K. nitens, but K. nitens lacks the central regulators of the canonical auxin-signaling pathway found in land plants. Exogenous IAA inhibited cell division and cell elongation in K. nitens. Inhibitors of auxin biosynthesis and of polar auxin transport also inhibited cell division and elongation. Moreover, exogenous IAA rapidly induced expression of a LATERAL ORGAN BOUNDARIES-DOMAIN transcription factor. These results suggest that K. nitens has acquired the part of the auxin system that regulates transcription and cell growth without the requirement for the central players that govern auxin signaling in land plants.

show abstract

“…However, intracellular gradients of calcium and auxin are difficult to follow during plant embryogenesis. Nevertheless, intracellular auxin gradient was detected directly in cells during proliferative period of Chara vulgaris [129] and this gradient might exist in embryonic cells as well.…”

Section: New Mechanism Regulating Intracellular Asymmetry and Cellulamentioning

confidence: 95%

The Winner Takes It All: Auxin—The Main Player during Plant Embryogenesis

Winnicki¹

2020

Cells

Self Cite

View full text Add to dashboard Cite

In plants, the first asymmetrical division of a zygote leads to the formation of two cells with different developmental fates. The establishment of various patterns relies on spatial and temporal gene expression, however the precise mechanism responsible for embryonic patterning still needs elucidation. Auxin seems to be the main player which regulates embryo development and controls expression of various genes in a dose-dependent manner. Thus, local auxin maxima and minima which are provided by polar auxin transport underlie cell fate specification. Diverse auxin concentrations in various regions of an embryo would easily explain distinct cell identities, however the question about the mechanism of cellular patterning in cells exposed to similar auxin concentrations still remains open. Thus, specification of cell fate might result not only from the cell position within an embryo but also from events occurring before and during mitosis. This review presents the impact of auxin on the orientation of the cell division plane and discusses the mechanism of auxin-dependent cytoskeleton alignment. Furthermore, close attention is paid to auxin-induced calcium fluxes, which regulate the activity of MAPKs during postembryonic development and which possibly might also underlie cellular patterning during embryogenesis.

show abstract

PIN2-like proteins may contribute to the regulation of morphogenetic processes during spermatogenesis in Chara vulgaris

Cited by 21 publications

References 60 publications

The Genome of the Charophyte AlgaPenium margaritaceumBears Footprints of the Evolutionary Origins of Land Plants

The Genome of the Charophyte AlgaPenium margaritaceumBears Footprints of the Evolutionary Origins of Land Plants

Primitive Auxin Response without TIR1 and Aux/IAA in the Charophyte Alga Klebsormidium nitens

The Winner Takes It All: Auxin—The Main Player during Plant Embryogenesis

Contact Info

Product

Resources

About