G2 Premature Chromosome Condensation/Chromosome Aberration Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology

Gotoh, Eisuke

doi:10.1007/978-1-4939-9432-8_6

Cited by 6 publications

(4 citation statements)

References 58 publications

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“…Alternatively, PCC can be induced chemically in PBL at any stage of the cell cycle by treatment with okadaic acid or calyculin, which are specific inhibitors of serine/threonine protein phosphatase [ 13 , 67 , 68 ]. The technique is very easy to practice, simply by substituting colcemid with either of the two chemicals.…”

Section: Employment Of Tc Staining Adds Distinctive Value To Commonly...mentioning

confidence: 99%

“…Such probes have the advantage of short hybridization times, high specificity, and signal intensity, in addition to low cost [ 10 ]. By employing telomere and centromere-specific PNA probes (TC staining), we have obtained significant advances in the scoring of dicentric and ring chromosomes—the best biomarkers of radiation-induced DNA damage—on metaphases from mitosis-induced lymphocytes [ 10 , 11 ] and also on premature chromosome condensation (PCC) in nonstimulated lymphocytes [ 12 , 13 ]. Furthermore, the application of TC staining to the CBMN assay has vastly improved the identification of micronucleus formation, thus increasing the applicability of this assay [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview

M’Kacher

Colicchio

Junker

et al. 2023

IJMS

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In the event of a radiological or nuclear accident, or when physical dosimetry is not available, the scoring of radiation-induced chromosomal aberrations in lymphocytes constitutes an essential tool for the estimation of the absorbed dose of the exposed individual and for effective triage. Cytogenetic biodosimetry employs different cytogenetic assays including the scoring of dicentrics, micronuclei, and translocations as well as analyses of induced premature chromosome condensation to define the frequency of chromosome aberrations. However, inherent challenges using these techniques include the considerable time span from sampling to result, the sensitivity and specificity of the various techniques, and the requirement of highly skilled personnel. Thus, techniques that obviate these challenges are needed. The introduction of telomere and centromere (TC) staining have successfully met these challenges and, in addition, greatly improved the efficiency of cytogenetic biodosimetry through the development of automated approaches, thus reducing the need for specialized personnel. Here, we review the role of the various cytogenetic dosimeters and their recent improvements in the management of populations exposed to genotoxic agents such as ionizing radiation. Finally, we discuss the emerging potentials to exploit these techniques in a wider spectrum of medical and biological applications, e.g., in cancer biology to identify prognostic biomarkers for the optimal triage and treatment of patients.

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Section: Employment Of Tc Staining Adds Distinctive Value To Commonly...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview

M’Kacher

Colicchio

Junker

et al. 2023

IJMS

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“…Estimating doses above 5 Gy using the DCA may prove challenging due to radiation-induced mitotic delay and extensive cell death. Consequently, obtaining the necessary number of cells for analysis and dose estimation may not be attainable [2,3].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, peripheral lymphocytes can undergo early chromosome condensation using okadaic acid and Calyculin-A [2,6].…”

Section: Introductionmentioning

confidence: 99%

Curva de calibración dosis-efecto para altas dosis de rayos X utilizando el ensayo de condensación prematura de cromosomas con Caliculina-A

Chaves-Campos,

Ortíz-Morales,

Mejías-Gamboa

et al. 2024

Braz. J. Rad. Sci.

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Purpose: This article shows the results of the Cytogenetics Laboratory of the Health Research Institute (INISA) to develop a dose-effect calibration curve with the Calyculin-A chemical induction premature condensation assay to estimate high doses of X-ray exposure. Methods: to create the calibration curve, peripheral blood samples from two participants (one female and one male) were exposed to X-rays at six different dose points ranging from 0 to 17.5 Gy in vitro. The irradiated blood was cultured for 48 hours according to international protocols, and the resulting chromosome rings were recorded. We used BioDoseTools software to calculate the coefficients for the calibration curve. Results: The coefficients of the curve are α: 0.028±0.001 and C: 0.001±0.001. These coefficients have similar values to those reported internationally. The curve was validated by calculating an unknown dose exposed to 6 Gy; the estimated dose was 5.651 ± 0.636 Gy, with no statistically significant differences between the dose delivered and the estimated dose. Conclusions: The INISA Biological Dosimetry Service can use the curve obtained to assess absorbed doses in cases of suspected overexposure to high X-ray doses.

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Multiwell-based G0-PCC assay for radiation biodosimetry

Royba,

Shuryak,

Ponnaiya

et al. 2024

Sci Rep

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G2 Premature Chromosome Condensation/Chromosome Aberration Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology

Cited by 6 publications

References 58 publications

High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview

High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview

Curva de calibración dosis-efecto para altas dosis de rayos X utilizando el ensayo de condensación prematura de cromosomas con Caliculina-A

Multiwell-based G0-PCC assay for radiation biodosimetry

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