The biological assay of vitamin D3

Jones, J. I. M.; Elliot, J.F.

doi:10.1042/bj0370209

Cited by 23 publications

(10 citation statements)

References 2 publications

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“…Feather keratins could be extracted with SDS solutions, in the presence of a reducing agent (19). The binding of a mixture of anionic surfactants, sodium alkylbenzenesulphonates, to reduced feather keratins was studied by Ward et al (20).…”

Section: Introductionmentioning

confidence: 99%

Stabilization of Solutions of Feather Keratins by Sodium Dodecyl Sulfate

Schrooyen

Dijkstra²,

Oberthür³

et al. 2001

Journal of Colloid and Interface Science

100

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Section: Introductionmentioning

confidence: 99%

Stabilization of Solutions of Feather Keratins by Sodium Dodecyl Sulfate

Schrooyen

Dijkstra²,

Oberthür³

et al. 2001

Journal of Colloid and Interface Science

100

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“…The Cestrum content in both diets was 3 g/100 g diet. Diet A contained 0.9% calcium and 0.7% phosphorus; diet B contained 1.45% calcium and 1.36% phosphorus [9]. The chicks were divided into three groups: one group ate diet A (Group 1); the second group had Cestrum mixed with diet A (Group 11); and the third group was given Cestrum in diet B (Group 111).…”

Section: Methodsmentioning

confidence: 99%

The Ultrastructure of Nephrocalcinosis Induced in Chicks by Cestrum diurnum Leaves

et al. 1981

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Abstract. Powdered Cesfrurn diurnum leaves, mixed with two diets differing in calcium and phosphorus contents, produced nephrocalcinosis in young chicks regardless of serum calcium elevation. The calcific deposits, found in both proximal and distal portions of cortical tubules, began either in the cytoplasm or in lysosomal bodies as a unilaminar spheroid structure containing apatite crystals. The ultrastructural characteristics of intraluminal concretions suggested that they were formed intracellularly but later were extruded into the lumen. The extent of calcific deposits increased with duration and with hypercalcemia. Although Cestrum contains an analog of 1,25-dihydroxycholecalciferol, neither mitochondria nor basal lamina contained calcific deposits described in nephroca1cinos:s secondary to hypervitaminosis D.The appearance of nephrocalcinosis following large doses of vitamin D was first reported in 1928 [14]. In recent years, great advances have been made in the understanding of vitamin D metabolism, and its biologically active metabolite has been isolated. We now know that vitamin D is converted in the liver into 25-hydroxycholecalciferol, which is the main circulating form of the vitamin in blood [3]. This metabolite is hydroxylated further in the kidney to 1,25-dihydroxycholeca1-ciferol, but this conversion is regulated closely according to the need of the organism.Thus, when excess vitamin D is given to normal animals, it is converted to 25-hydroxycholecalciferol without subsequent production of 1,25-dihydroxycholecalciferol. Consequently, the serum of animals with experimental hypervitaminosis D shows a high concentration of 25-hydroxycholecalciferol, while the concentration of 1,25-dihydroxycholecalciferol remains relatively low [4].It has been established that 1,25-dihydroxycholecalciferol is the most active metabolite of vitamin D [3, 41, but induction of nephrocalcinosis by pure 1,25-dihydroxycholecalciferol has not been reported. Renal calcification, however, is seen frequently among animals feeding on plants such as Solunum mulucoxylon [2] and Trisetumflavescens [6], which contain an analog of 1,25-dihydroxycholecalciferol. On the other hand, neither cattle nor horses feeding on Cesrrzim diurnum, another plant 62

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“…Power for the various units is taken from a main drive with variable-pitch pulleys for speed regulation. [22]. Feathers can be dispersed in alkaline sulfide solutions.…”

mentioning

confidence: 99%

“…Following analysis for nitrogen, a factor of 6.21 is used to calculate the protein-Nacconol ratio. Because a good spinning &dquo;dope&dquo; contains about 45 parts of feather keratin to 55 parts of Nacconol and because the powdered complex is a little higher in protein, a small calculated amount of Nacconol is added to the necessary amount of water and enough complex is added to make a dope -containing 22 For precipitation of the mechanically extruded protein-detergent complexes, a saturated solution of magnesium sulfate, adjusted to pH 1 to 2 with sulfuric acid, is effective. The precipitation is determined by cations and follows the order predicted by the Schulze-Hardy rule.…”

mentioning

confidence: 99%

Synthetic Protein Fibers from Protein-Detergent Complexes

Lundgren

1945

Textile Research Journal

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The biological assay of vitamin D3

Cited by 23 publications

References 2 publications

Stabilization of Solutions of Feather Keratins by Sodium Dodecyl Sulfate

Stabilization of Solutions of Feather Keratins by Sodium Dodecyl Sulfate

The Ultrastructure of Nephrocalcinosis Induced in Chicks by Cestrum diurnum Leaves

Synthetic Protein Fibers from Protein-Detergent Complexes

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