Stabilization of Solutions of Feather Keratins by Sodium Dodecyl Sulfate

Schrooyen, P.M.M.; Dijkstra, Pieter J.; Oberthür, R. C.; Bantjes, A.; Feijén, Jan

doi:10.1006/jcis.2001.7673

Cited by 100 publications

(84 citation statements)

References 27 publications

(28 reference statements)

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“…Sulfur is known to be an important structural component of sulfhydryl amino acids such as methionine, cystine and cysteine, which are essential for the formation of keratin, the main constituent of the hoof capsule. These amino acids are linked by disulfide bonds, forming a very tough three-dimensional network (Schrooyen et al, 2001), which partly explains the higher levels of this element in the digits that bear a greater portion of the animal's weight.…”

Section: Discussionmentioning

confidence: 99%

Biochemical composition of the hoof capsule of buffaloes and its influence on hoof quality

Assis

Vulcani

Silva

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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The purpose of this study was to establish the biochemical parameters of the abaxial wall, dorsal wall and sole of the hoof of the medial thoracic, lateral, and medial pelvic digits of buffalos. The hoof samples were subjected to destructive biochemical analyses to identify the dry material (DM), mineral matter (MM), organic matter (OM), crude protein (CP) and ether extract (EE) contents. Sulfur (S), calcium (Ca), potassium (K), phosphorus (P), zinc (Zn) and copper (Cu) levels were determined based on nondestructive biochemical analyses. The parameters of dry material, mineral matter, organic matter, crude protein and ether extract of hoof capsule of the digits of buffalos can be determined by means of both destructive and nondestructive biochemical analysis. In addition, this study revealed that the highest concentrations of DM, CP and minerals such as, K, Zn and Cu are concentrated in the digits that bear the greatest body mass weight, suggesting that there is a positive correlation between the aforementioned parameters and the strength and growth of the hoof capsule in the digits. As for the element S, this study demonstrated that its highest concentration is located in the lateral digits of the pelvic members.Keywords: buffaloes, keratinized epidermis, minerals, crude protein RESUMO O presente estudo objetivou estabelecer os parâmetros bioquímicos da muralha abaxial, muralha dorsal e sola do casco dos dígitos torácico medial e pélvico lateral dos bubalinos. Foram realizadas analises bioquímicas destrutivas das amostras dos cascos para se obterem os teores de matéria seca (MS), matéria mineral (MM), matéria orgânica (MO), proteína bruta (PB) e extrato etéreo (EE). As análises bioquímicas não destrutivas foram empregadas para se avaliarem os níveis de enxofre (S), cálcio (Ca), potássio (K), fósforo (P), zinco (Zn) e cobre (Cu

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Section: Discussionmentioning

confidence: 99%

Biochemical composition of the hoof capsule of buffaloes and its influence on hoof quality

Assis

Vulcani

Silva

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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“…Reduction of keratin by 2-mercaptoethanol, dithiothreitol (DTT) or dithioerythritol, thioglycolic acid, glutathione, sulphites, and bisulphite generates free cysteine residues, and the resulting cysteine-containing derivatives are called ''kerateines'' [7,[15][16][17][18]. They are less polar and more stable in acidic and alkaline solutions than the oxidized derivatives, and they contain amino acid residues capable of re-crosslinking.…”

Section: Introductionmentioning

confidence: 99%

“…As the majority of the keratin remains trapped within the protective structures, during this reaction a denaturing solvent, such as urea, thiourea, transition metal hydroxides, surfactant solutions, and combination thereof should be applied, to limit the effects of ionic and hydrogen bonds interaction [16,20]. In turn, addition of sodium dodecylsulfate (SDS) provides faster and more efficient extraction by formation of complexes keratin-SDS, and prevents protein aggregation [15]. The reduction process is industrially usable.…”

Section: Introductionmentioning

confidence: 99%

Alternative Methods of Preparation of Soluble Keratin from Chicken Feathers

Sinkiewicz

Śliwińska

Staroszczyk

et al. 2016

Waste Biomass Valor

139

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Huge amount of keratinous waste, especially birds' feathers, demands more value-added application instead of dumping. The present work reports the results of experiments aimed at preparing soluble keratin useful for novel bioproduct formation. The effect of thermo-chemical treatments with various reducing agents, i.e. 2-mercaptoethanol, dithiothreitol, sodium m-bisulphite, and sodium bisulphite, as well as sodium hydroxide, on the yield of keratin extracted from chicken feathers was determined. It was shown that after 2-h reduction with 2-mercaptoethanol and sodium bisulphite, the yield of soluble keratin was about equal and amounted to 84 and 82 %, respectively. The cheaper and harmless sodium bisulphite additionally decreased the extraction time to 1 h with the same yield. Moreover, treatment of the feathers with 2.5 % NaOH further improved the extraction effectiveness by increasing the yield up to 94 %. The results of the study demonstrate the viability of hydrolytic processes to obtain soluble keratin useful for biodegradable film formation for food application, that are harmless and more effective than solubilization by reduction of the disulphide bonds. Graphical Abstract

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“…Moreover, fibre formation is promoted at low polymer concentrations, increasing solution conductivity (Son et al, 2004). A great increase in conductivity was measured from solutions containing the keratin because of SDS that remains associated with keratin through ionic interaction conferring negative charges to the protein (Schrooyen et al, 2001). In fact the conductivity of pure PEO was about 0.120 mS cm -1 and the conductivity of keratin/PEO was 1.282 mS cm -1 (at polymer concentration 7% wt).…”

Section: Solution Characterizationmentioning

confidence: 99%