The biofilm matrix of<i>Campylobacter jejuni</i>determined by fluorescence lectin-binding analysis

Turonova, Hana; Neu, Thomas R.; Ulbrich, Pavel; Pazlarová, J.; Tresse, Odile

doi:10.1080/08927014.2016.1169402

Cited by 22 publications

(15 citation statements)

References 57 publications

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“…Biofilm was stained simultaneously with fluorescent probes: Syto9 in green for cell detection and two fluorescently labeled lectins Concanavaline-A (ConA) in blue to detect a-D-mannosyl and a-D-glucosyl residues and wheat-germ agglutinin lectin (WGA) in red to detect N-acetylglucosamine (GlcNAc) residues. For details see Turonova et al (2016) (see also video S1) # Hana Turo nov a. Reuse not permitted. Delatolla et al, 2009;Hannig et al, 2010;Karcz et al, 2012;Pompilio et al, 2015).…”

Section: Microscopy Methodsmentioning

confidence: 99%

“…Being noninvasive, CLSM represents the methods of choice for the distribution and in situ characterization analyses of EPS (Bhardwaj et al, 2013;Neu & Lawrence, 2014b;Watrous & Dorrestein, 2011). Identification of the EPS carbohydrates could be approached using fluorescence lectinbinding analysis (FLBA) which detects glycoconjugates and their distribution within the biofilm (Figure 10(C), and S1) (Marchal et al, 2011;Neu & Lawrence, 2014bTuronova et al, 2016;Zippel & Neu, 2011). Characterization of EPS carbohydrate using FLBA depends on the specificity of the lectins used (Weissbrodt et al, 2013;Zhang et al, 2015a).…”

Section: Measurement Of Eps Componentsmentioning

confidence: 99%

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Critical review on biofilm methods

Azeredo

Azevedo

Briandet

et al. 2016

Critical Reviews in Microbiology

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Biofilms are widespread in nature and constitute an important strategy implemented by microorganisms to survive in sometimes harsh environmental conditions. They can be beneficial or have a negative impact particularly when formed in industrial settings or on medical devices. As such, research into the formation and elimination of biofilms is important for many disciplines. Several new methodologies have been recently developed for, or adapted to, biofilm studies that have contributed to deeper knowledge on biofilm physiology, structure and composition. In this review, traditional and cutting-edge methods to study biofilm biomass, viability, structure, composition and physiology are addressed. Moreover, as there is a lack of consensus among the diversity of techniques used to grow and study biofilms. This review intends to remedy this, by giving a critical perspective, highlighting the advantages and limitations of several methods. Accordingly, this review aims at helping scientists in finding the most appropriate and up-to-date methods to study their biofilms. ARTICLE HISTORY

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Section: Microscopy Methodsmentioning

confidence: 99%

Section: Measurement Of Eps Componentsmentioning

confidence: 99%

Critical review on biofilm methods

Azeredo

Azevedo

Briandet

et al. 2016

Critical Reviews in Microbiology

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760

679

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“…FLBA has been employed to study the matrix architecture in a variety of different biofilms, including environmental, industrial, and medical biofilms [15–23]. After an initial screening step where the binding of a large number of fluorescently labeled lectins to a particular type of biofilm is tested, selected lectins exhibiting high affinity to the biofilms in question are employed to visualize and quantify specific glycoconjugate motifs [24].…”

Section: Introductionmentioning

confidence: 99%

Visualizing the dental biofilm matrix by means of fluorescence lectin-binding analysis

Tawakoli

Neu

Busck

et al. 2017

Journal of Oral Microbiology

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The extracellular matrix is a poorly studied, yet important component of dental biofilms. Fluorescence lectin-binding analysis (FLBA) is a powerful tool to characterize glycoconjugates in the biofilm matrix. This study aimed to systematically investigate the ability of 75 fluorescently labeled lectins to visualize and quantify extracellular glycoconjugates in dental biofilms. Lectin binding was screened on pooled supragingival biofilm samples collected from 76 subjects using confocal microscopy. FLBA was then performed with 10 selected lectins on biofilms grown in situ for 48 h in the absence of sucrose. For five lectins that proved particularly suitable, stained biovolumes were quantified and correlated to the bacterial composition of the biofilms. Additionally, combinations of up to three differently labeled lectins were tested. Of the 10 lectins, five bound particularly well in 48-h-biofilms: Aleuria aurantia (AAL), Calystega sepiem (Calsepa), Lycopersicon esculentum (LEA), Morniga-G (MNA-G) and Helix pomatia (HPA). No significant correlation between the binding of specific lectins and bacterial composition was found. Fluorescently labeled lectins enable the visualization of glycoconjugates in the dental biofilm matrix. The characterization and quantification of glycoconjugates in dental biofilms require a combination of several lectins. For 48-h-biofilms grown in absence of sucrose, AAL, Calsepa, HPA, LEA, and MNA-G are recommendable.

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“…It was observed that approximately 80% of bacterial infections are related to biofilm formation because these polymeric structures give them good protection against antibiotics, disinfectants, the host immune system, and stressful environmental conditions . The biofilm‐producing capacity of Salmonella and Campylobacter strains isolated from different sources in the food supply chain is well known . This is a cause for concern in the food industry owing to the major public health problem it represents.…”

Section: Introductionmentioning

confidence: 99%

Salmonella and Campylobacter biofilm formation: a comparative assessment from farm to fork

et al. 2018

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It takes several steps to bring food from the farm to the fork (dining table), and contamination with food-borne pathogens can occur at any point in the process. Campylobacter spp. and Salmonella spp. are the main microorganisms responsible for foodborne disease in the EU. These two pathogens are able to persist throughout the food supply chain thanks to their ability to form biofilms. Owing to the high prevalence of Salmonella and especially of Campylobacter in the food supply chain and the huge efforts of food authorities to reduce these levels, it is of great importance to fully understand their mechanisms of persistence. Diverse studies have evaluated the biofilm-forming capacity of foodborne pathogens isolated at different steps of food production. Nonetheless, the principal obstacle of these studies is to reproduce the real conditions that microorganisms encounter in the food supply chain. While there are a wide number of Salmonella biofilm studies, information on Campylobacter biofilms is still limited. A comparison between the two microorganisms could help to develop new research in the field of Campylobacter biofilms. Therefore, this review evaluates relevant work in the field of Salmonella and Campylobacter biofilms and the applicability of the data obtained from these studies to real working conditions. © 2018 Society of Chemical Industry.

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The biofilm matrix ofCampylobacter jejunidetermined by fluorescence lectin-binding analysis

Cited by 22 publications

References 57 publications

Critical review on biofilm methods

Critical review on biofilm methods

Visualizing the dental biofilm matrix by means of fluorescence lectin-binding analysis

Salmonella and Campylobacter biofilm formation: a comparative assessment from farm to fork

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