The AtRAD21.1 and AtRAD21.3 Arabidopsis cohesins play a synergistic role in somatic DNA double strand break damage repair

Costa-Nunes, José A. da; Capitão, Cláudio; Kozák, Jaroslav; Costa‐Nunes, Pedro; Ducasa, G. Michelle; Pontes, Olga; Angelis, Karel J.

doi:10.1186/s12870-014-0353-9

Cited by 18 publications

(16 citation statements)

References 67 publications

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“…This protein has a specific function in DNA repair in Arabidopsis somatic cells and, unlike other cohesin complex subunits, its absence does not affect sister chromatid cohesion ( Schubert et al, 2009 ). A similar function has also been recently described for SYN4 (AtRAD21.3), which has synergistic and non-redundant effect on the SYN2 function ( da Costa-Nunes et al, 2014 ). We confirmed that the increase in AtPDS5 transcripts is due to a specific role in DSB repair and not a consequence of a general deregulation produced by DNA damage by means of analyzing hypersensitivity to different DNA damage agents.…”

Section: Discussionmentioning

confidence: 53%

Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana

et al. 2015

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Maintenance and precise regulation of sister chromatid cohesion is essential for faithful chromosome segregation during mitosis and meiosis. Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle. One of these, PDS5, also known as SPO76, is essential during mitosis and meiosis in several organisms and also plays a role in DNA repair. In yeast, the complex Wapl-Pds5 controls cohesion maintenance and colocalizes with cohesin complexes into chromosomes. In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained. Here we have isolated mutants for each of the five AtPDS5 genes (A–E) and obtained, after different crosses between them, double, triple, and even quadruple mutants (Atpds5a Atpds5b Atpds5c Atpds5e). Depletion of AtPDS5 proteins has a weak impact on meiosis, but leads to severe effects on development, fertility, somatic homologous recombination (HR) and DNA repair. Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex. Contrarily to its function in other species, our results suggest that AtPDS5 is dispensable during the meiotic division of Arabidopsis, although it plays an important role in DNA repair by HR.

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Section: Discussionmentioning

confidence: 53%

Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana

et al. 2015

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“…This is consistent with anchoring of chromatin loops to the axis via REC8-cohesin causing local exclusion of the recombination machinery. Cohesin has a role in DSB repair and can antagonize interhomolog recombination by promoting intersister repair of DSBs (Yoon et al, 2016;Hong et al, 2013;da Costa-Nunes et al, 2014;Bolaños-Villegas et al, 2017). Hence, in Arabidopsis it is also possible that high levels of REC8 associate with promotion of inter-sister repair, which could contribute to the low crossover frequency observed in the heterochromatic pericentromeres.…”

Section: Discussionmentioning

confidence: 99%

Interacting Genomic Landscapes of REC8-Cohesin, Chromatin, and Meiotic Recombination in Arabidopsis

et al. 2020

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“…When mutated, the number of DSBs increases prior to and after DNA damage, and repair is severely delayed. Additional loss of KU80, a component of NHEJ, exacerbates this repair deficiency, suggesting a role of SMCs during early phases of DSB repair, likely for initiating de novo cohesion between sister chromatids to facilitate HR (Kozak et al, 2009;da Costa-Nunes et al, 2014). AtMMS21, interacting with SMC5, participates in DNA damage reduction in the root stem cell niche to preserve genome integrity .…”

Section: Chromatin-remodeling Complexes Connected With Ddrmentioning

confidence: 99%

DNA Damage Repair in the Context of Plant Chromatin

Donà

Scheid

2015

Plant Physiol.

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The integrity of DNA molecules is constantly challenged. All organisms have developed mechanisms to detect and repair multiple types of DNA lesions. The basic principles of DNA damage repair (DDR) in prokaryotes and unicellular and multicellular eukaryotes are similar, but the association of DNA with nucleosomes in eukaryotic chromatin requires mechanisms that allow access of repair enzymes to the lesions. This is achieved by chromatin-remodeling factors, and their necessity for efficient DDR has recently been demonstrated for several organisms and repair pathways. Plants share many features of chromatin organization and DNA repair with fungi and animals, but they differ in other, important details, which are both interesting and relevant for our understanding of genome stability and genetic diversity. In this Update, we compare the knowledge of the role of chromatin and chromatin-modifying factors during DDR in plants with equivalent systems in yeast and humans. We emphasize plant-specific elements and discuss possible implications.A DNA molecule in a eukaryotic chromosome has a diameter of 2 nm but a length in the range of centimeters. Being 10 7 times longer than it is wide would make it highly sensitive to breakage if it were not organized in compact and dynamic chromatin, with nucleosomes as basic units followed by multiple higher order levels of organization. Within this packaging, replication and transcription constitute an endogenous mechanical strain, while exposure of cells to physical or chemical hazard creates a wide range of DNA damage induced by external factors, including photoproducts, pyrimidine dimers, interstrand crosslinking, and single and double-strand breaks (DSBs). All organisms possess mechanisms that repair DNA molecules. DNA damage repair (DDR) systems for the various types of damage are overlapping as well as complementary, and their prevalence depends on the organism, the stage of the cell cycle, the site and the amount of damage, and the availability of intact templates. A coarse categorization distinguishes nucleotide excision repair, base excision repair, nonhomologous end joining (NHEJ), and homologous recombination (HR). Many schematic models for these DDR pathways describe the action and interaction of several repair components, but they are usually misleading in one aspect: DNA strands are illustrated as straight lines, neglecting the emerging role of chromatin for the location and the fate of DNA lesions. More realistically, we should envisage DNA lesions in the chromatin context like the leakage or burst of an in-ground pipe. Recognizing the defect, localizing it, excavating the broken parts, cleaning the ends, reconnecting them, and restoring the original state are mirrored in the subsequent steps of DDR: signaling, labeling, accessing, resecting, religating, and reassembling. Chromatin is expected to especially affect the access, resection, and restoration of the original arrangement, and several factors that can dissolve higher order structures, slide, evict, or exchange ...

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The AtRAD21.1 and AtRAD21.3 Arabidopsis cohesins play a synergistic role in somatic DNA double strand break damage repair

Cited by 18 publications

References 67 publications

Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana

Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana

Interacting Genomic Landscapes of REC8-Cohesin, Chromatin, and Meiotic Recombination in Arabidopsis

DNA Damage Repair in the Context of Plant Chromatin

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