Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana

Abstract

Maintenance and precise regulation of sister chromatid cohesion is essential for faithful chromosome segregation during mitosis and meiosis. Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle. One of these, PDS5, also known as SPO76, is essential during mitosis and meiosis in several organisms and also plays a role in DNA repair. In yeast, the complex Wapl-Pds5 controls cohesion maintenance and colocalizes with cohesin complexes into chromosomes. In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained. Here we have isolated mutants for each of the five AtPDS5 genes (A–E) and obtained, after different crosses between them, double, triple, and even quadruple mutants (Atpds5a Atpds5b Atpds5c Atpds5e). Depletion of AtPDS5 proteins has a weak impact on meiosis, but leads to severe effects on development, fertility, somatic homologous recombination (HR) and DNA repair. Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex. Contrarily to its function in other species, our results suggest that AtPDS5 is dispensable during the meiotic division of Arabidopsis, although it plays an important role in DNA repair by HR.

PDS 5 proteins regulate the length of axial elements and telomere integrity during male mouse meiosis

“…Analyses of meiosis in Pds5 mutants in Sordaria , budding and fission yeasts, and the worm Caenorhabditis elegans , indicate that this protein is involved in several processes including cohesion, formation of AEs/LEs, condensation, homolog pairing, and repair of DSBs . In contrast, simultaneous ablation of four out of the five Pds5 genes present in Arabidopsis thaliana does not have a relevant impact in meiotic chromosome structure, or the progression of meiosis . In mammalian meiosis, like in mitosis, both PDS5A and PDS5B are present.…”

Identification and Characterization of Phosphoproteins in Somatic Embryogenesis Acquisition during Oil Palm Tissue Culture

“…In our study, the sister chromatid cohesion protein PDS5 homolog A isoform X2 was specifically expressed in the globular, torpedo and plantlet stages that regulate chromosome segregation during mitosis and meiosis and was also found to play a role in DNA repair. The depletion of AtPDS5 proteins has a weak impact on meiosis but leads to severe effects on development, fertility, somatic homologous recombination (HR), and DNA repair [28]. In our study, ten proteins were expressed only in the embryogenic callus acquisition, somatic embryo, and plantlet stages.…”

The Human Tyrosyl-DNA Phosphodiesterase 1 (hTdp1) Inhibitor NSC120686 as an Exploratory Tool to Investigate Plant Tdp1 Genes

“…These were the following: PARP1, HEN2 (RNA helicase, ATP-dependent, SK12/DOB1 protein), Top1 , ATM (Ataxia Telangiectasia Mutated serine/threonine-kinase), MRE11 , FZR3 (FIZZY-related 3), CYCB2-4 (Cyclin B2;4), AT3G07010 (pectin lyase-like superfamily), AT2G33440 (RNA-binding RRM/RBD/RNP family), AT1G15940 (Tudor/PWWP/MBT superfamily), AT1G34355 (Parallel Spindel 1 PS1 fork-associated (FDA) domain containing protein), AT2G21560 (nucleolar-like protein), AT1G72250 (di-glucose binding protein Kinesin motor domain-containing protein), AT3G51280 (tetratricopeptide (TPR)-like superfamily), AT5G51720 (WEB family containing the DUF827 domain), AT5G10940 (ASG2 transducin family protein, WD40 repeat family). Among them, PARP1 , HEN2 , Top1 , ATM , MRE11 , and AT1G15940 [ 28 ], have known roles in DNA repair. The resulting list contains additional accessions because the GeneMANIA software extends the user’s list to include genes that are functionally similar or that have shared properties with the initial query genes [ 26 ].…”