Interacting Genomic Landscapes of REC8-Cohesin, Chromatin, and Meiotic Recombination in Arabidopsis

Lambing, Christophe; Tock, Andrew J.; Topp, Stephanie D.; Choi, Kyuha; Kuo, Pallas; Zhao, Xiaohui; Osman, Kim; Higgins, James D.; Franklin, F. Christopher H.; Henderson, Ian

doi:10.1105/tpc.19.00866

Cited by 57 publications

(92 citation statements)

References 96 publications

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“…In mammals, distinct TADs and loops are formed by cohesin through loop extrusion until CCCTC-binding factor (CTCF) is encountered 38 . Arabidopsis REC8 (a core component of cohesin) is enriched in both centromeres and heterochromatin during meiosis 39 . When REC8 is depleted, many transposons are activated 39 .…”

Section: Discussionmentioning

confidence: 99%

“…Arabidopsis REC8 (a core component of cohesin) is enriched in both centromeres and heterochromatin during meiosis 39 . When REC8 is depleted, many transposons are activated 39 . While CTCF has no direct homolog in plants, rice Hi-C analysis revealed that the plant-specific proteins TCP and bZIP recognize motifs embedded in TADs and contribute to the formation of TADs 3,40 .…”

Section: Discussionmentioning

confidence: 99%

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Heat stress-induced transposon activation correlates with 3D chromatin organization rearrangement in Arabidopsis

et al. 2020

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In higher eukaryotes, heterochromatin is mainly composed of transposable elements (TEs) silenced by epigenetic mechanisms. But, the silencing of certain heterochromatin-associated TEs is disrupted by heat stress. By comparing genome-wide high-resolution chromatin packing patterns under normal or heat conditions obtained through Hi-C analysis, we show here that heat stress causes global rearrangement of the 3D genome in Arabidopsis thaliana. Contacts between pericentromeric regions and distal chromosome arms, as well as proximal intra-chromosomal interactions along the chromosomes, are enhanced. However, interactions within pericentromeres and those between distal intra-chromosomal regions are decreased. Many inter-chromosomal interactions, including those within the KNOT, are also reduced. Furthermore, heat activation of TEs exhibits a high correlation with the reduction of chromosomal interactions involving pericentromeres, the KNOT, the knob, and the upstream and downstream flanking regions of the activated TEs. Together, our results provide insights into the relationship between TE activation and 3D genome reorganization.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Heat stress-induced transposon activation correlates with 3D chromatin organization rearrangement in Arabidopsis

et al. 2020

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“…REC8 occupancy in such regions suggests that REC8 could suppress meiotic DSBs. Lambing et al (2020) examined this possibility by mapping REC8-enriched regions to previously reported meiotic DSB hotspots and crossover sites. REC8 and nucleosome colocalization indeed associates with meiotic DSB suppression.…”

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confidence: 99%

“…Immunostaining of HA-tagged REC8 and DNA (DAPI) show similar REC8 enrichment at chromocenters (white arrows) in kyp suvh5 suvh6 and wild type male meiocytes. (The right panel is reproduced from Lambing et al [2020], Figure 6. )…”

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The Rules of Attachment: REC8 Cohesin Connects Chromatin Architecture and Recombination Machinery in Meiosis

Shahid

2020

Plant Cell

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Crossover patterning in plants

Lloyd

2022

Plant Reprod

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Key message Chromatin state, and dynamic loading of pro-crossover protein HEI10 at recombination intermediates shape meiotic chromosome patterning in plants. Abstract Meiosis is the basis of sexual reproduction, and its basic progression is conserved across eukaryote kingdoms. A key feature of meiosis is the formation of crossovers which result in the reciprocal exchange of segments of maternal and paternal chromosomes. This exchange generates chromosomes with new combinations of alleles, increasing the efficiency of both natural and artificial selection. Crossovers also form a physical link between homologous chromosomes at metaphase I which is critical for accurate chromosome segregation and fertility. The patterning of crossovers along the length of chromosomes is a highly regulated process, and our current understanding of its regulation forms the focus of this review. At the global scale, crossover patterning in plants is largely governed by the classically observed phenomena of crossover interference, crossover homeostasis and the obligatory crossover which regulate the total number of crossovers and their relative spacing. The molecular actors behind these phenomena have long remained obscure, but recent studies in plants implicate HEI10 and ZYP1 as key players in their coordination. In addition to these broad forces, a wealth of recent studies has highlighted how genomic and epigenomic features shape crossover formation at both chromosomal and local scales, revealing that crossovers are primarily located in open chromatin associated with gene promoters and terminators with low nucleosome occupancy.

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Interacting Genomic Landscapes of REC8-Cohesin, Chromatin, and Meiotic Recombination in Arabidopsis

Abstract: One-sentence summary: REC8 association with the genome correlates with multiple chromatin states and is required to organize meiotic chromosome architecture and interhomolog recombination.

Cited by 57 publications

References 96 publications

Heat stress-induced transposon activation correlates with 3D chromatin organization rearrangement in Arabidopsis

Heat stress-induced transposon activation correlates with 3D chromatin organization rearrangement in Arabidopsis

The Rules of Attachment: REC8 Cohesin Connects Chromatin Architecture and Recombination Machinery in Meiosis

Crossover patterning in plants

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