The assessment of blood copper status in cattle: A comparison of measurements of caeruloplasmin and elemental copper in serum and plasma

Laven, Richard; Lawrence, K.E.; Livesey, C. T.

doi:10.1080/00480169.2007.36763

Cited by 33 publications

(21 citation statements)

References 11 publications

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“…In cows, serum inorganic phosphate (Pi) concentrations were slightly higher than plasma Pi, but both were still highly correlated (Montiel et al, 2007). Copper was reported to be lower (about 3 mmol/l) in serum with high inter-individual variation (Laven et al, 2007).…”

Section: Technique-related Changesmentioning

confidence: 98%

Pre-analytical factors affecting the results of laboratory blood analyses in farm animal veterinary diagnostics

Humann-Ziehank

Ganter

2012

Animal

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The quality of the laboratory diagnostic approach in farm animals can be severely affected by pre-analytical factors of variation. They induce increase/decrease of biochemical and hematological analyte concentrations and, as a consequence, they may cause unsuitable conclusions and decisions for animal health management and research projects. The pre-analytical period covers the preparation of sampling, the sampling procedure itself, as well as all specimen handling until the beginning of the specific laboratory analysis. Pre-analytical factors may have either an animal-related or a technique-related background. Animal-related factors cover daytime/season, meals/fasting, age, gender, altitude, drugs/anesthesia, physical exercise/stress or coinfection. Technique-related factors are the choice of the tube including serum v. plasma, effects of anticoagulants/gel separators, the anticoagulant/blood ratio, the blood collection procedure itself, specimen handling, contamination, labeling, storage and serum/plasma separation, transportation of the specimen, as well as sample preparation before analysis in the laboratory. It is essential to have proper knowledge about the importance and source of pre-analytical factors to alter the entire diagnostic process. Utmost efforts should be made to minimize controllable factors. Analytical results have to be evaluated with care considering that pre-analytical factors of variation are possible causes of misinterpretation.

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Section: Technique-related Changesmentioning

confidence: 98%

Pre-analytical factors affecting the results of laboratory blood analyses in farm animal veterinary diagnostics

Humann-Ziehank

Ganter

2012

Animal

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“…Furthermore, it undergoes physiological changes during different lactation stages of dairy cows [ 14 ]. In addition, Cp has a potentiality for evaluation of copper status in dairy cows [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Analytical validation of bovine plasma ceruloplasmin measurement by p-phenylenediamine oxidation and effect of storage time and freezing temperature

Hussein

Staufenbiel

2017

Acta Vet Scand

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BackgroundDetermination of ceruloplasmin (Cp) activity in plasma can provide an objective measure of the health of dairy cows as well as it can be used for various diagnostic purposes. The current study was designed to perform an analytical validation of a method for the determination of plasma Cp activity in dairy cows and to evaluate the influences of plasma storage times and temperatures as well as freeze–thaw cycles on the activity of this enzyme. This cohort was carried out on ten cows. For each cow, 24 aliquots of plasma, which were stored at different temperature regimes, were prepared. Both intra- and interassay coefficients of variation (CVs) were determined. The linearity was evaluated using bovine plasma Cp standard.ResultsThe mean values of intra- and interassay CVs were 1.08 and 2.12%, respectively. Results of linearity testing showed a high correlation coefficient (r = 0.998, P < 0.001). After 3 days of storage at room temperature and refrigeration, the plasma activity of Cp was significantly lowered (P < 0.05). Plasma samples kept at freezing for 3 months revealed insignificant changes in the activity of Cp. Repeated freeze–thaw cycles for three times had no significant influence on the activity of Cp.ConclusionsThe method is easy and may be valid at values of Cp ranging from 100 to 1000 mg/L. It seems that keeping of plasma samples at room temperature and refrigeration longer than 3 days is unsuitable for Cp measurement. In addition, Cp remains stable in plasma samples stored at freezing as well as repeat freeze–thaw cycles.

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“…Contrary, higher level of P had beef animals. The absorption, accumulation and toxicity of each element may be affected by diverse factors, including interactions with others (GALLO et al 1996, LAVEN et al 2007. There have been many reports of interactions between Cu and other elements in cattle.…”

Section: Discussionmentioning

confidence: 99%

Effect of selected factors on mineral parameters in plasma of cows

2010

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Abstract. Objective of this paper was to evaluate the effects of herd, exploitation, management system, and season on blood macro and micro-minerals in cows kept in a mountainous region. The cows were kept in four herds, two exploitation manners, and two systems. Concentrations of calcium (Ca), phosphorus (P) were significantly differed in the factors of herd and exploitation only, levels of copper (Cu) and zinc (Zn) showed differences in all factors. Beef animals had higher level of P than dairy exploitation (1.59 mg.L−1 vs. 1.42 mg.L−1; P<0.01) and lower level of Ca (2.10 mg.L−1 vs. 2.33 mg.L−1; P<0.001). Higher concentrations of Cu and Zn were recorded in dairy exploitation (0.91 mg.L−1, 1.17 mg.L−1) versus beef exploitation (0.67 mg.L−1, 0.86 mg.L−1) (P<0.001). We found higher concentrations in Cu (0.84 mg.L−1 vs. 0.62 mg.L−1 , P<0.001) and Zn (1.06 mg.L−1 vs. 0.96 mg.L−1, P<0.05) in non-ecological system. There were higher values of Cu and Zn in fall than spring (0.90 mg.L−1 and 1.10 mg.L−1 vs. 0.67 mg.L−1 and 0.93 mg.L−1) (P<0.001).

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The assessment of blood copper status in cattle: A comparison of measurements of caeruloplasmin and elemental copper in serum and plasma

Abstract: The use of serum Cu concentration rather than plasma Cu concentration in the diagnosis of Cu responsive disease in cattle needs to be re-evaluated as does the way in which individual sample results are used in such tests.

Cited by 33 publications

References 11 publications

Pre-analytical factors affecting the results of laboratory blood analyses in farm animal veterinary diagnostics

Pre-analytical factors affecting the results of laboratory blood analyses in farm animal veterinary diagnostics

Analytical validation of bovine plasma ceruloplasmin measurement by p-phenylenediamine oxidation and effect of storage time and freezing temperature

Effect of selected factors on mineral parameters in plasma of cows

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