The application of magnets directs the orientation of neurite outgrowth in cultured human neuronal cells

S, Kim; Im, Wooseok; Kang, Lami; Lee, Sang Kun; Chu, Kon; Kim, Byoung In

doi:10.1016/j.jneumeth.2008.07.005

Cited by 48 publications

(33 citation statements)

References 24 publications

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“…However, in this study, the proliferation of C2C12 cells was also inhibited with the application of magnets. Magnetic field induces differentiation and the outgrowth of neurites in cultured human neuronal cells [19]. Thus, magnetic fields may contribute to the differentiation of muscle cells, which may delay the rate of cell cycle progression.…”

Section: Discussionmentioning

confidence: 99%

Static Magnetic Fields Inhibit Proliferation and Disperse Subcellular Localization of Gamma Complex Protein3 in Cultured C2C12 Myoblast Cells

Kim

2010

Cell Biochem Biophys

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Magnetic fields may delay the rate of cell cycle progression, and there are reports that magnetic fields induce neurite outgrowth in cultured neuronal cells. To demonstrate whether magnetic field also effects on myoblast cells in cell growth, C2C12 cell lines were cultured and 2000G static magnetic field was applied. After 48 h of incubation, both the WST-1 assay (0.01 < P < 0.025, t-test) and direct cell counting (P < 0.0005, t-test) showed that static magnetic fields inhibit the proliferation of cultured C2C12 cells. Immunocytochemistry for alpha and tubulin gamma complex protein (TUBA and GCP3) was made and applying a static magnetic field-dispersed tubulin GCP3 formation, a intracellular apparatus for tubulin structuring in cell division. This protein expression was not altered by western blot. This study indicates that applying a static magnetic field alters the subcellular localizing of GCP3, and may delay the cell growth in cultured C2C12 myoblast cells.

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Section: Discussionmentioning

confidence: 99%

Static Magnetic Fields Inhibit Proliferation and Disperse Subcellular Localization of Gamma Complex Protein3 in Cultured C2C12 Myoblast Cells

Kim

2010

Cell Biochem Biophys

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“…Although high amplitude Ca 2+ influxes were observed in the latter case, 154 and intracellular distribution of proteins 54,155 and suggest the use of wireless magnetic forces to remotely modulate neural network activity.…”

Section: Introductionmentioning

confidence: 90%

“…Kim and colleagues also reported the use of static magnetic fields to elicit neurite outgrowth in PC-12 cells and the orientation of neurite growth was perpendicular to the direction of the applied magnetic field. 154 The team found that magnetic field could polarize the localization of actin and microtubules but did not change the levels of synaptotagmin responsible for synaptic formations. Nonetheless, in these papers, the authors recognize that they did not demonstrate a connection between signaling cascades and the reported morphological change in neurite outgrowth, which we attempt to answer in our piece of research.…”

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confidence: 99%

Acute and Chronic Neural Stimulation via Mechano-Sensitive Ion Channels

Tay

2018

Springer Theses

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Neural stimulation techniques for eliciting calcium influx can elucidate the physiological roles of specific neural populations. To overcome some of the limitations of existing techniques such as poor specificity and noxious effects of heat, I developed a technology for non-invasive control of neural activities using magnetic forces and magnetic nanoparticles (MNPs) which offer deep tissue penetration and controllable dosage. Extensive investigations with different neurotoxins and experimental conditions support that the mechanism of magnetic stimulation that involves membrane-bound MNPs transducing magnetic forces into mechanical stretching of the cell membrane to enhance the opening probability of mechano-sensitive N-type calcium ion channels to induce calcium influx.Making use of the ability of neural networks to actively regulate their ratio of excitatory to inhibitory ion channel/receptor, I also performed chronic magnetic stimulation on fragile X iii syndrome (FXS) model neural networks. We found that chronic magnetic stimulation reduced the density of N-type calcium ion channels whose expression is increased in FXS. This technique demonstrates the potential of using bio-magnetic/mechanical forces to modulate expressions of mechano-sensitive ion channels where they are over-expressed in diseases such as abnormal nociception.Nonetheless, there are still a few areas where the technique can be improved. Firstly, it is the use of MNPs with more uniform properties to have greater control on magnetic stimulations.Secondly, the technique needs to be useful for in vivo studies. Therefore, I started researching on magnetotactic bacteria (MTB) which produce biological MNPs with superior properties such as uniform sizes and highly homogenous magnetic properties with the goal of harvesting MNPs from them.MTB, however, grow extremely slowly and the number of MNPs produced/bacterium is low. One way to overcome this problem is to evolve MTB over-producers of MNPs but this strategy is constrained by the absence of a selection platform that is quantitative and offer high throughput. To overcome this problem, I combined random chemical mutagenesis and selection using a magnetic ratcheting platform to generate and isolate MTB over-producers that produce twice as many MNPs/bacterium after 5 rounds of mutation/selection. I next designed a magnetic microfluidic device and demonstrate as a proof of concept, that it can be coupled to a bioreactor for high throughput microfluidic selection of MTB over-producers.iv

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“…Previous studies had reported that PEMFs are able to modify some parameters of nerve function in diabetic patients and can stimulate nerve growth, regeneration, and functional recovery of nerves in cells in animal models with nerve disease [56,57].…”

Section: Doi: 107243/2055-2386-4-17mentioning

confidence: 99%

Influence of low level laser therapy versus pulsed electromagnetic field on diabetic peripheral neuropathy

Mohamed¹,

Abdallah²,

Abdeen³

et al. 2017

Phys Ther Rehabil

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Background: Peripheral neuropathy is a common complaint of diabetes, leading to pain and reduced motor nerve conduction velocity. Clinical symptoms of peripheral neuropathy are present in approximately 25% of diabetic individuals, while nearly all diabetics have a reduction of nerve conduction velocity. Purpose: This study aimed to evaluate and compare the effect of low-level laser therapy (LLLT) versus pulsed electromagnetic field (PEMF) on pain intensity and motor nerve conduction velocity (MNCV) in patients with diabetic neuropathy. Methods: Thirty patientswith type II diabetes suffering from diabetic peripheral neuropathy, participated in this study for 4 weeks (3 sessions/week), and were chosen randomly from the diabetes and endocrine institution.Patients were randomized equally into two groups: Group A (LLLT group): received LLLT for lower extremities for 12 sessions at a frequency of 3 sessions/week. Group B (PEMF): received pulsed electromagnetic field for 12 sessions at a frequency of 3 sessions/week. Results: At the end of the study; there was non-significant difference between two groups post-study in pain level where P-values was (0.606). There were no significant differences between two groups in amplitude, distal latency and MNCV of RT side post-study, where P-values were (0.082), (0.911) and (0.342) respectively. There were no significant differences between two groups in amplitude, distal latency and MNCV of LT side post-study, where P-values were (0.265), (0.550) and (0.334) respectively. Conclusions: The study findings indicate that both LLLT andPEMF could be effective therapeutic modalities in the treatment of painful diabetic neuropathy in that they are able to modify pain, and some electrophysiological parameters of peripheral nerve function.

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The application of magnets directs the orientation of neurite outgrowth in cultured human neuronal cells

Cited by 48 publications

References 24 publications

Static Magnetic Fields Inhibit Proliferation and Disperse Subcellular Localization of Gamma Complex Protein3 in Cultured C2C12 Myoblast Cells

Static Magnetic Fields Inhibit Proliferation and Disperse Subcellular Localization of Gamma Complex Protein3 in Cultured C2C12 Myoblast Cells

Acute and Chronic Neural Stimulation via Mechano-Sensitive Ion Channels

Influence of low level laser therapy versus pulsed electromagnetic field on diabetic peripheral neuropathy

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