The Application of Erythromycin, Elbayou, and Rica-1 Probiotic in the Rearing of Scylla paramamosain Mud Crab Larvae Development into the Crablet Stage

Potential role of prebiotics and probiotics in conferring health benefits in economically important crabs

“… In vitro study Inhibition of the growth of bacteria isolated from the shell of hermit crab Gallini et al [66] Bacillus subtilis S. paramamosain (5.2 g) 10 5 cfu/g diet, 30 days Immunity, disease resistance Wu et al [8] B. subtilis E20 S. paramamosain (juvenile) 10 8 , 10 9 , 10 10 cfu/g diet Growth performance, immunity, disease resistance Yeh et al [4] Lactobacillus plantarum P. pelagicus (larvae) 1.0 × 10 6 , 5.0 × 10 6 and 1.0 × 10 7 cfu/mL culture water, 14 days Survival rate, digestive enzyme activities, water quality, inhibition of pathogen growth Talpur et al [67] L. plantarum 7–40 S. paramamosain (juvenile) 10 9 cfu/g diet Growth performance, immunity, disease resistance Yeh et al [4] Enterococcus faecalis Y17 S. paramamosain (100±3 g) 10 9 cfu/g diet, 6 weeks Growth performance, serum enzyme activities, disease resistance Yang et al [42] Pediococcus pentosaceus G11 S. paramamosain (100±3 g) 10 9 cfu/g diet, 6 weeks Growth performance, serum enzyme activities, disease resistance Yang et al [42] Five strains of Alteromonadaceae (2 strains), Flavobacteriaceae (1), Aeromonadaceae (1), Alteromonadaceae (2) Scylla serrata (larvae) N/A Survival rate, suppression of the necrosis Dan and Hamasaki [68] BreviB. brevis (Rica-1) S. paramamosain (larvae) 15 mg/L culture water, 25 days Survival rate, suppression of pathogen growth Gunarto et al [70] ...…”

“…Previous studies have shown that probiotics can be supplied directly through the feed to introduce live cells to the gut of host animals helping to improve digestive function, growth, or immune system responses [ 8 , 42 ] or applied directly to culture systems to improve water quality and the survival of cultured animals [ 65 , 67 , 70 ]. Feeding period is an important factor continuously keeping the presence of probiotics in the gastrointestinal tract of the animals.…”

“…The commercial probiotic product (Rica-1) containing Gram-positive BreviBacillus brevis has been previously reported to inhibit the growth of Vibrio spp. in Artemia [70] . In mud crab ( S. paramamosain ) larvae, the effectiveness of probiotic (Rica-1), antibiotics (erythromycin), and anti-bacterial nitrofuran chemotherapy (elbayou) (with a dose of 15 mg/L rearing water each) in the rearing tanks of mud crab larvae was investigated in detail [70] .…”

“…in Artemia [70] . In mud crab ( S. paramamosain ) larvae, the effectiveness of probiotic (Rica-1), antibiotics (erythromycin), and anti-bacterial nitrofuran chemotherapy (elbayou) (with a dose of 15 mg/L rearing water each) in the rearing tanks of mud crab larvae was investigated in detail [70] . The results showed that the application of Rica-1 probiotic is less effective in the crablet production and suppression of Vibrio harveyi infecting the megalopa when compared to the use of erythromycin and elbayou.…”

“…No crablet was yielded in the rearing tank on the 20th day of rearing and the megalopa was died by the infection of V. harveyii in the Rica-1 probiotic-added rearing tank. The reason for this may be due to the thinning process between tanks to minimize cannibalism (from the zoea-5 to megalopa period) causing stress conditions of the larvae [70] .…”