The application of BMRT-HPV viral load to secondary screening strategies for cervical cancer

Duan, Lyufang; Du, Hui; Wang, Chun; Huang, Xia; Qu, Xiaoyan; Shu, Bin; Liu, Yan; Zhang, Wei; Duan, Xiaohui; Wei, Lihui; Belinson, Jerome L.; Wu, Ruifang

doi:10.1371/journal.pone.0232117

Cited by 19 publications

(21 citation statements)

References 14 publications

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“…No similar undertaking could be done for the CmPV1 results as there are no comparable datasets in marine turtles. However, the results are consistent with human cervical intraepithelial neoplasia (CIN) I, II, III caused by human papillomavirus (HPV) 16, 18, 33 and 58 [ 48 , 49 , 50 ].…”

Section: Resultssupporting

confidence: 57%

“…While the mean ChHV5 load results obtained in this study were consistent with other reports [ 23 , 25 , 45 , 46 ], such comparisons could not be made for CmPV1 as there are no other comparable datasets in marine turtles. However, the CmPV1 viral loads are in the same range reported for human cervical malignancies caused by HPV 16, 18, 33 and 58 where the mean HPV copies per 10,000 cells were reported to be ~log3–5 in CIN I, II and III [ 48 , 49 , 50 ].…”

Section: Discussionmentioning

confidence: 52%

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The Concurrent Detection of Chelonid Alphaherpesvirus 5 and Chelonia mydas Papillomavirus 1 in Tumoured and Non-Tumoured Green Turtles

Mashkour

Jones

Wirth

et al. 2021

Animals

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Characterised by benign tumours, fibropapillomatosis (FP) is a debilitating disease that predominantly afflicts the endangered green turtle (Chelonia mydas). A growing body of histological and molecular evidence has associated FP tumours with Chelonid alphaherpesvirus 5 (ChHV5). However, a recent study which detected both ChHV5 and Chelonia mydas papillomavirus 1 (CmPV1) DNA in FP tumour tissues has challenged this hypothesis. The present study aimed to establish a probe-based qPCR to assess the wider prevalence of CmPV1 and co-occurrence with ChHV5 in 275 marine turtles foraging in waters adjacent to the east coast of Queensland, Australia: three categories: Group A (FP tumours), Group B (non-tumoured skin from FP turtles) and Group C (non-tumoured skin from turtles without FP). Concurrent detection of ChHV5 and CmPV1 DNA is reported for all three categories, where Group A had the highest rate (43.5%). ChHV5 viral loads in Group A were significantly higher than loads seen in Group B and C. This was not the case for CmPV1 where the loads in Group B were highest, followed by Group A. However, the mean CmPV1 load for Group A samples was not significantly different to the mean load reported from Group B or C samples. Collectively, these results pivot the way we think about FP; as an infectious disease where two separate viruses may be at play.

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Section: Resultssupporting

confidence: 57%

Section: Discussionmentioning

confidence: 52%

The Concurrent Detection of Chelonid Alphaherpesvirus 5 and Chelonia mydas Papillomavirus 1 in Tumoured and Non-Tumoured Green Turtles

Mashkour

Jones

Wirth

et al. 2021

Animals

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“…Women testing HPV positive by either Cobas or SeqHPV (self or direct) were referred for colposcopy, using the quadrant-based POI (Preventive Oncology International Inc.) protocol of directed and random biopsies plus ECC [8]. The implementation and methods of the trial have been detailed in prior publications [9,10].…”

Section: Study Populationmentioning

confidence: 99%

The prevalence and distribution of human papillomavirus among 10,867 Chinese Han women

Guo

Belinson

et al. 2021

Infect Agents Cancer

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Objective To assess the prevalence and distribution of HPV genotypes among Chinese Han women, and to explore the risk of high-grade cervical lesions associated with individual hr-HPV genotypes. Methods Genotyping and histopathology data from the Chinese Multi-Center Screening Trial (CHIMUST) and its pilot screening trial, from 6 regions across mainland China, were re-analyzed. The data from physician- and self-collected samples from 10,867 Chinese Han women (ages 30–69) were used to determine the prevalence and distribution of hr-HPV and to explore the risk association between hr-HPV genotypes and precancerous lesions. Results 9.2% of the study population tested hr-HPV positive in physician-collected sample. The prevalence varied regionally from the lowest in Guangdong (6.3%) to the highest in Inner Mongolia (13.0%). The most prevalent genotypes found were HPV-52 (21.7%), HPV-16 (19.2%), HPV-58 (15.0%), HPV-39 (8.9%), and HPV-51 (8.2%). The overall odds ratios for CIN2+ and CIN3+ for the presence of HPV-16 was 58.6 (95% CI 39.2–87.5) and, 91.6 (95%CI 54.3–154.6), respectively and remained the highest odds ratio for CIN3+ in all 6 regions. Conclusion Geographical variation exists in the prevalence and distribution of hr-HPV in mainland China. HPV-16/52/58 were the most prevalent genotypes, and HPV-16 had the highest risk for high-grade cervical lesions. Trial registration CHIMUST, Registration number: ChiCTR-EOC-16008456. Registered 11 May 2016.

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“…While the mean ChHV5 load results obtained in this study were consistent with other reports [24,44,45], such comparisons could not be made for CmPV1 as there are no other comparable datasets in marine turtles. However, the CmPV1 viral loads are in the same range reported for human cervical malignancies caused by HPV 16, 18, 33 and 58 where the mean HPV copies per 10,000 cells were reported to be ~log3-5 in CIN I, II and III [47][48][49]. Additional molecular surveys targeting both ChHV5 and CmPV1 from other regions would be hugely beneficial to understanding the role of CmPV1 in FP, and in marine turtle health as a whole.…”

Section: Mean Viral Loadmentioning

confidence: 64%

The Concurrent Detection of Chelonid Alphaherpesvirus 5 and Chelonia mydas Papillomavirus 1 in Tumoured and Non-Tumoured Green Turtles

Mashkour

Jones

Wirth

et al. 2021

Preprint

View full text Add to dashboard Cite

Characterised by the growth of benign tumours, fibropapillomatosis (FP) is a debilitating disease that predominantly afflicts the endangered green turtle (Chelonia mydas). A growing body of histological and molecular evidence has consistently associated FP tumours with Chelonid alphaherpesvirus 5 (ChHV5), leading this virus to be considered the most likely aetiological agent of FP. However, a recent study which detected both ChHV5 and Chelonia mydas papillomavirus 1 (CmPV1) DNA in FP tumour tissues has challenged this hypothesis. The present study aimed to establish the wider prevalence of CmPV1 and co-occurrence with ChHV5 in marine turtles in waters adjacent to the east coast of Queensland, Australia. This comprehensive molecular survey screened a total of 353 samples from 275 foraging turtles using probe-based qPCR. Three sample categories were used in this study: Group A (FP tumours), Group B (non-tumoured skin from turtles with FP tumours) and Group C (non-tumoured skin from turtles without FP tumours). Concurrent detection of ChHV5 and CmPV1 DNA is reported for all three categories, with the highest rate of concurrent detection reported for Group A samples (43.5%). Collectively, these results pivot the way we think about FP; as an infectious disease where two separate viruses may be at play.

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The application of BMRT-HPV viral load to secondary screening strategies for cervical cancer

Cited by 19 publications

References 14 publications

The Concurrent Detection of Chelonid Alphaherpesvirus 5 and Chelonia mydas Papillomavirus 1 in Tumoured and Non-Tumoured Green Turtles

The Concurrent Detection of Chelonid Alphaherpesvirus 5 and Chelonia mydas Papillomavirus 1 in Tumoured and Non-Tumoured Green Turtles

The prevalence and distribution of human papillomavirus among 10,867 Chinese Han women

The Concurrent Detection of Chelonid Alphaherpesvirus 5 and Chelonia mydas Papillomavirus 1 in Tumoured and Non-Tumoured Green Turtles

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