The apparent requirement of two hormones, α- and β-ecdysone, for molting induction in insects

Clever, Ulrich; Clever, Ingrid; Storbeck, Ingrid; Young, Nancy L.

doi:10.1016/0012-1606(73)90320-5

Cited by 66 publications

(10 citation statements)

References 28 publications

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“…Subsequent work by Clever et al (1973) has shown that this puff induction also occurs in vitro. When we set out to reproduce these experiments we encountered the problem that puff 1-18C could be induced in some cases and not in others.…”

Section: Introductionmentioning

confidence: 89%

Developmental changes in the responsiveness to ecdysterone of chromosome region I-18C of Chironomus tentans

1989

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Abstract. The presence of nascent RNA in chromosome region I-I 8C was revealed by the method of induced hybrid formation (IEH) and by use of anti-RNA: DNA antibodies. IEH signals were quantitated, and used as a measure of transcriptional activity in the region. The expansion of the 1-18C region was also determined and used as an indication of local chromatin decondensation. Chironomus tentans larvae may undergo oligopause or they may develop subitaneously. It was found that the course of basal activity in 1-18C differs substantially in these two modes of development. Most interestingly they also differ in activation of 1-18C in response to in vitro ecdysterone treatment. Changes in hormonal responsiveness of 1-18C parallel changes in the preexisting state of 1-18C decondensation. Oligopause stage 5 animals, being most refractory in this respect, exhibit the most condensed 1-18C chromosome region.

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Section: Introductionmentioning

confidence: 89%

Developmental changes in the responsiveness to ecdysterone of chromosome region I-18C of Chironomus tentans

1989

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“…In contrast in Drosophila, ~-ecdysone showed only 2-3% of the efficiency of B-ecdysone in the induction of puffing changes. Of particular interest in this connection is the difference in sensitivity and reaction to c~-and/~-ecdysone of the early puffs 1-18-C and IV-2-B of C. tentans which Clever et al (1973) demonstrated by injection and in vitro experiments. In vitro, 1-18-C could only be induced by ~-ecdysone, not by fl-ecdysone; IV-2-B reacts within a few minutes to /% ecdysone and to ~-ecdysone with a considerable delay.…”

Section: Developmental Puffing Changesmentioning

confidence: 98%

Developmental puffing activity in the salivary gland and Malpighian tubule chromosomes ofAcricotopus lucidus (Diptera, Chironomidae)

Staiber

Behnke

1985

Chromosoma

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Abstract.A detailed map of the salivary gland chromosomes of Acricotopus lucidus is presented. Differences in puffing and developmental puffing sequences of the three salivary gland lobes were investigated from mid fourth larval instar to pupation and compared with the puffing pattern of the Malpighian tubules. The intraglandular differentiation is quite extensive; the differences in the pattern of gene activity between the anterior lobe and the main and side lobes are as great as between the salivary gland and the Malpighian tubules. In the main and side lobes all developmental puffing changes proceed synchronously whereas in the anterior lobe both asynchronous and synchronous changes occur. In the anterior lobe the asynchronous regression of BR 3 and BR 4 is followed by a characteristic sequence of activation and inactivation of puffs.

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“…1 The very first evidence that it may have an intrinsic effect came from puffing experiments in Chironomus tentans in which the “early puff” I‐18‐C is activated more quickly by E than by 20E. 7 In Drosophila , E exhibits only low activity chromosome puffing in salivary glands but proves almost as effective as 20E in inducing the same puffs in fat body cells. 8 The response of some fat body loci to E occurs even more rapidly.…”

Section: Evidence For the Possible Activities Of Ecdysteroids Other Tmentioning

confidence: 99%

“…Beside E, 3D20E, and the 20‐hydroxylated ecdysteroids just mentioned, no other reported zooecdysteroid seems to have strong intrinsic biological activity, except for an unidentified metabolite of 20E which caused chromosome puffing in Chironomus . 7 Early studies proposed a possible role for the metabolites 26E and 20,26E, 15 but recent studies with Manduca epidermis showed that these compounds were inactive in regulating various genes, including EcR. 9…”

Section: Evidence For the Possible Activities Of Ecdysteroids Other Tmentioning

confidence: 99%

Functional Significance of Ecdysteroid Diversity in Arthropods^a

Böcking

Hiruma

1998

Annals of the New York Academy of Sciences

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The apparent requirement of two hormones, α- and β-ecdysone, for molting induction in insects

Cited by 66 publications

References 28 publications

Developmental changes in the responsiveness to ecdysterone of chromosome region I-18C of Chironomus tentans

Developmental changes in the responsiveness to ecdysterone of chromosome region I-18C of Chironomus tentans

Developmental puffing activity in the salivary gland and Malpighian tubule chromosomes ofAcricotopus lucidus (Diptera, Chironomidae)

Functional Significance of Ecdysteroid Diversity in Arthropods^a

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The apparent requirement of two hormones, α- and β-ecdysone, for molting induction in insects

Cited by 66 publications

References 28 publications

Developmental changes in the responsiveness to ecdysterone of chromosome region I-18C of Chironomus tentans

Developmental changes in the responsiveness to ecdysterone of chromosome region I-18C of Chironomus tentans

Developmental puffing activity in the salivary gland and Malpighian tubule chromosomes ofAcricotopus lucidus (Diptera, Chironomidae)

Functional Significance of Ecdysteroid Diversity in Arthropodsa

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Functional Significance of Ecdysteroid Diversity in Arthropods^a