Developmental puffing activity in the salivary gland and Malpighian tubule chromosomes ofAcricotopus lucidus (Diptera, Chironomidae)

Abstract

Abstract.A detailed map of the salivary gland chromosomes of Acricotopus lucidus is presented. Differences in puffing and developmental puffing sequences of the three salivary gland lobes were investigated from mid fourth larval instar to pupation and compared with the puffing pattern of the Malpighian tubules. The intraglandular differentiation is quite extensive; the differences in the pattern of gene activity between the anterior lobe and the main and side lobes are as great as between the salivary gland and the Malpighian tubules. In the main and side lobes all developmental puffing changes proceed synchronously whereas in the anterior lobe both asynchronous and synchronous changes occur. In the anterior lobe the asynchronous regression of BR 3 and BR 4 is followed by a characteristic sequence of activation and inactivation of puffs.

Characterization of heterochromatin of germ line limited and soma chromosomes in Acricotopus lucidus (Diptera, Chironomidae) by differential banding methods

“…The K heterochromatin insertions were highly vacuolated. In contrast, the heterochroniatic centromere regions of the polytene Ss frequently exhibit distinct banding patterns (STAIBER and BEHNKE 1985). In an earlier investigation it was demonstrated that centromeric S bands contain sequences that could be activated to fomi a Balbiani ring when they are translocated into euchromatic chromosome sections (STAIBER 1982).…”

“…2). In contrast to the Ks, each of the three Ss shows only one obvious centsomeric C-band, which corresponds to the large heterochromatic block representing the centromeric region in the polytene salivary gland chromosome (STAIBER and BEHNKE 1985). To facilitate the recognition of chromosomes and the comparison of banding patterns, a set of Gbanded Ks and Ss is shown in Fig.…”

Chironomus group classification according to the mapping of polytene chromosomes

“…However, this approach is limited and less implemented in Malaysia. Polytene chromosomes are present in several dipteran tissues, including the salivary glands, malpighian tubules, and the epithelium of the midgut and hindgut [2,3]. There are several distinct characteristics which aid in classifying the group of chromosome arrangement.…”

Immunofluorescence Study of Spindle Microtubule Arrangements during Differential Gonial Mitosis of Acricotopus lucidus(Diptera, Chironomidae).

“…Animals of a laboratory stock of Acricotopus lucidus (Diptera, Chironomidae) were used. Rearing conditions and development of the larvae were described earlier (16).…”