Immunofluorescence Study of Spindle Microtubule Arrangements during Differential Gonial Mitosis of Acricotopus lucidus(Diptera, Chironomidae).

Abstract

ABSTRACT. The changes in microtubule arrangements correlated with the behavior of the germ line limited and soma chromosomes were studied during the last unequal gonial mitosis, the so-called differential mitosis, of the chironomid Acricotopus lucidus by indirect immunofluorescence using a monoclonal anti-^tubulin antibody, and by simultaneous staining with the DNA-specific fluorescence dye DAPI. An impressive difference in microtubule density between both half spindles was determined in metaphase and during the monopolar anaphasic migration of the germ line limited chromosomes. In the following normal separation of the soma chromosomes, a similar microtubule density in both half spindles occurred. In each of the half spindles, chromosome movementand spindle elongation occurred independently, and in one half spindle two anaphasic chromosome movements ran off one after another, the second without a simultaneous spindle elongation.

Asymmetric distribution of mitochondria and of spindle microtubules in opposite directions in differential mitosis of germ line cells in Acricotopus

“…A first analysis of the sequential changes of the spindle MTs in differential mitosis of Acricotopus was performed on fixed spermatogonial cells by indirect immunofluorescence with an anti-beta-tubulin antibody (Staiber 1994). In the present study, the use of fluorochrome-labelled paclitaxel (taxol) on living cells has allowed a more detailed study of MT arrangements and of sequential changes of the spindle in differential mitosis.…”

Spermatogenesis in Tenebrio molitor (Tenebrionidae, Coleoptera): A Fine Structure and Anti‐tubulin Immunofluorescence Study

“…Nicklas & Gordon 1985; Swedak et a f . 1991) and Diptera (Staiber 1994). It should, however, be pointed out that this is not the rule in the animal realm.…”