The Apoptotic Effect of Green Tea (−)-Epigallocatechin Gallate on 3T3-L1 Preadipocytes Depends on the Cdk2 Pathway

Wu, Ben-Zen; Hung, Pei Fang; Chen, Hui Chian; Huang, Rong; Chang, Hsin Huei; Kao, Ying-Jer

doi:10.1021/jf050045p

Cited by 79 publications

(63 citation statements)

References 38 publications

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“…Since apoptosis occurs in a very structured manner, upstream molecules, such as caspases, can indicate cell death before it would be visually apparent. Caspase-3 concentrations were orders of magnitude less than the levels reported in similar cells where apoptosis occurred [12], suggesting that apoptosis was not increased by the material treatments.…”

Section: Discussionmentioning

confidence: 59%

Adult adipose-derived stem cell attachment to biomaterials

2007

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Attachment of adipose-derived stem cells (ASC) to biomaterials prior to implantation is a possible strategy for mediating inflammation and wound healing. In this study, the ASC percent coverage was measured on common medical grade biosensor materials subjected to different surface treatments. Cell coverage on silicone elastomer (poly dimethylsiloxane) was below 20% for all surface treatments. Polyimide (Kapton), polyurethane (Pellethane) and tissue culture polystyrene all exhibited >50% coverage for surfaces treated with fibronectin (Fn), fibronectin plus avidin/biotin (dual ligand), and oxygen plasma plus fibronectin treatments (Fn O 2 ). The fibronectin treatment performed as well or better on polyimide, polyurethane, and tissue culture polystyrene compared to the dual ligand and fibronectin oxygen plasma treated surfaces. Cell detachment with increasing shear stresses was <25% for each attachment method on both polyimide and polyurethane. The effects of attachment methods on the basic cell functions of proliferation, metabolism, ATP concentration, and caspase-3 activity were analyzed yielding proliferation profiles that were very similar among all of the materials. No significant differences in metabolism, intracellular ATP, or intracellular caspase-3 activity were observed for any of the attachment methods on either polyimide or polyurethane.

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Section: Discussionmentioning

confidence: 59%

Adult adipose-derived stem cell attachment to biomaterials

2007

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“…Effects of tea catechins on anti-obesity are well documented. For example, EGCg, a major tea catechin, suppressed differentiation of 3T3-L1 preadipocytes to mature adipocytes Wolfram et al, 2006) and induced apoptosis (Wu et al, 2005). In animal experiPrevention of Hyperglycemia by Black Tea Fig.…”

Section: Discussionmentioning

confidence: 99%

Concentration of Catechins and Caffeine in Black Tea Affects Suppression of Fat Accumulation and Hyperglycemia in High-fat Diet-fed Mice

Imada

Tanaka

Nishiumi

et al. 2011

FSTR

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In this study, we investigated preventive effects of black tea on obesity and hyperglycemia in C57BL/6 mice fed a high-fat diet for 14 weeks. During the feeding period, mice were given the following black teas: BT1 containing 3,000 mg/L total catechins and 864 mg/L caffeine; and BT2 containing 1,437 mg/L total catechins and 594 mg/L caffeine. Both BT1 and BT2 suppressed high-fat diet-caused body weight gain, deposition of white adipose tissue, and increases in plasma lipids and glucose. Moreover, both BT1 and BT2 counteracted the high-fat diet-caused decrease in the expression of insulin receptor (IR)-β and glucose transporter 4 (GLUT4), markers for insulin resistance. Of these effects, BT2 were stronger than that of BT1. In conclusion, black tea prevents hyperglycemia and insulin resistance through maintenance of IR-β and GLUT4 expression in high-fat diet-fed mice, with an appropriate concentration of tea producing maximal effectiveness.

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“…3. According to a previous report, Hsu et al 43) demonstrated that gallic acid could induce apoptotic cell death in 3T3-L1 preadipocytes in a similar way to EGCg, 44) which results in cell population growth being correlated with antioxidant activity. Additionally, Rosso et al 45) reported that gallic acid n-alquil esters have a protective effect on targets of HOCl, a powerful oxidant, through the inhibition of its productive enzyme, MPO activity, and HOCl scavenging.…”

Section: Effect Of Gs On Renal Glucose Intracellular Glycation and mentioning

confidence: 96%

7-O-Galloyl-D-sedoheptulose Is a Novel Therapeutic Agent against Oxidative Stress and Advanced Glycation Endproducts in the Diabetic Kidney

Yamabe

Kang

Park

et al. 2009

Biological & Pharmaceutical Bulletin

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The Apoptotic Effect of Green Tea (−)-Epigallocatechin Gallate on 3T3-L1 Preadipocytes Depends on the Cdk2 Pathway

Cited by 79 publications

References 38 publications

Adult adipose-derived stem cell attachment to biomaterials

Adult adipose-derived stem cell attachment to biomaterials

Concentration of Catechins and Caffeine in Black Tea Affects Suppression of Fat Accumulation and Hyperglycemia in High-fat Diet-fed Mice

7-O-Galloyl-D-sedoheptulose Is a Novel Therapeutic Agent against Oxidative Stress and Advanced Glycation Endproducts in the Diabetic Kidney

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