Adult adipose-derived stem cell attachment to biomaterials

Abstract

Attachment of adipose-derived stem cells (ASC) to biomaterials prior to implantation is a possible strategy for mediating inflammation and wound healing. In this study, the ASC percent coverage was measured on common medical grade biosensor materials subjected to different surface treatments. Cell coverage on silicone elastomer (poly dimethylsiloxane) was below 20% for all surface treatments. Polyimide (Kapton), polyurethane (Pellethane) and tissue culture polystyrene all exhibited >50% coverage for surfaces treated with fibronectin (Fn), fibronectin plus avidin/biotin (dual ligand), and oxygen plasma plus fibronectin treatments (Fn O 2 ). The fibronectin treatment performed as well or better on polyimide, polyurethane, and tissue culture polystyrene compared to the dual ligand and fibronectin oxygen plasma treated surfaces. Cell detachment with increasing shear stresses was <25% for each attachment method on both polyimide and polyurethane. The effects of attachment methods on the basic cell functions of proliferation, metabolism, ATP concentration, and caspase-3 activity were analyzed yielding proliferation profiles that were very similar among all of the materials. No significant differences in metabolism, intracellular ATP, or intracellular caspase-3 activity were observed for any of the attachment methods on either polyimide or polyurethane.

Extracellular Matrix Molecules Enhance the Neurotrophic Effect of Schwann Cell-Like Differentiated Adipose-Derived Stem Cells and Increase Cell Survival Under Stress Conditions

“…This result could be explained by the high affinity of stem cells for TCPS. 39,40 Similar studies showed no significant proliferation difference between TCPS and fibronectin coating. 40 Hence, dASC behave differently from primary SC, which show enhanced proliferation on fibronectin and laminin.…”

“…39,40 Similar studies showed no significant proliferation difference between TCPS and fibronectin coating. 40 Hence, dASC behave differently from primary SC, which show enhanced proliferation on fibronectin and laminin. 34,41 ECM molecules protect dASC from induced apoptosis and anoikis Cell adhesion to ECM is important for survival, differentiation, and migration of cells, 16 and cell attachment to biomaterials and survival is important for regenerative cell therapy.…”

Functional endothelialized microvascular networks with circular cross-sections in a tissue culture substrate

“…In the first method, two embossed polystyrene parts were treated with oxygen plasma (Prichard et al 2007) using a Technics Microstripper, with a setting of 100 W and 100 mTorr O 2 pressure for 1 min. The treated surfaces were placed in contact, and the channels were aligned with the aid of an optical microscope.…”

Osteogenesis of Adipose-Derived Stem Cells

“…Many studies have combined biomaterial scaffolds with ASCs to repair bone defects, for example, biphasic calcium phosphate nanocomposite (NanoBCP) (102), poly (lactic-co-glycolic acid) (PLGA) (103), HA/tricalcium phosphate (TCP) (104), chitooligosaccharide (COS) (105), fibrin/HA (106), and so on. Prichard et al (107) examined cell coverage and cell function of ASCs on different biomaterials, including fibronectin, silicone rubber, dualligand, polyimide, oxygen plasma plus fibronectin and polyurethane. The results showed that cell attachment was very strong on both polyurethane and polyimide for all attachment methods.…”