The antioxidant, rather than prooxidant, activities of quercetin on normal cells:quercetin protects mouse thymocytes from glucose oxidase-mediated apoptosis

Lee, Jeong-Chae; Kim, Ju; Park, Sang-Youel; Chung, Gook-Hyun; Jang, Yong-Suk

doi:10.1016/s0014-4827(03)00410-5

Cited by 98 publications

(59 citation statements)

References 52 publications

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“…The extract (20 NaOH and 1 mL of 2.8% solution of trichloroacetic acid (TCA), the mixtures were heated at 100°C for 15 min, and then cooled on ice. Absorbance of the samples was measured at 532 nm [19]. Rutin was used as the control.…”

Section: Hydroxyl Radical Scavenging Activitymentioning

confidence: 99%

Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pančić and Vis. (Apiaceae)

et al. 2013

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The chemical composition, antimicrobial and antioxidant activity of Eryngium palmatum, an endemic plant species from the Balkan Peninsula, were investigated. The flavonoids apigenin (9.5±0.3 mg g−1) and apigenin 7-O-glucoside (2.4±0.1 mg g−1) were determined in a methanol extract of aerial parts using HPLC analysis. The methanol extract of roots contained catechin (5.0±0.1 mg g−1), epicatechin (2.9±0.1 mg g−1), chlorogenic acid (1.6±0.0 mg g−1), gallic acid (0.9±0.0 mg g−1) and rosmarinic acid (0.9±0.2 mg g−1). GC-FID and GCMS analysis of a chloroform extract of aerial parts showed that the main volatile constituents were falcarinol, linoleic acid, hexadecanoic acid and methyl linoleate (comprising 32.6%; 24.4%; 19.9; 13.2% of the volatile fraction, respectively), while octanoic acid, tetradecanol and dodecanol dominated in the chloroform extract of the roots (34.9%; 25.8%; 22.2% of the volatile fraction, respectively). Investigation of antimicrobial activity by broth microdilution showed that the methanol and chloroform extracts of aerial parts and roots exerted a significant effect (MIC 3.5–15.6 μg mL−1) against tested Gram-positive and Gram-negative bacteria. The methanol extracts of aerial parts or roots exerted moderate ferric reducing antioxidant power, DPPH radical scavenging activity and hydroxyl radical scavenging activity.

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Section: Hydroxyl Radical Scavenging Activitymentioning

confidence: 99%

Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pančić and Vis. (Apiaceae)

et al. 2013

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“…Quercetin is a flavonoid and an important polyphenolic antioxidant ( Fig. 1) (3). It was reported that quercetin was able to maintain blood-brain barrier integrity by scavenging active oxygen (4).…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective effects of quercetin in a mouse model of brain ischemic/reperfusion injury via anti-apoptotic mechanisms based on the Akt pathway

Chao

Zhang

et al. 2015

Molecular Medicine Reports

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Abstract. The present study provided experimental evidence for the neuroprotective effects of quercetin using a rat model of global brain ischemic/reperfusion (I/R) injury. Pre-treatment with quercetin (5 or 10 mg/kg orally (p.o.); once daily) induced a dose-dependent reduction in I/R-induced hippocampal neuron cell loss, with 10 mg/kg/day being the lowest dose that achieved maximal neuroprotection. Administration of 10 mg/kg quercetin over at least 3 days prior to I/R was required to improve the survival rate of I/R rats. Fluorescence-assisted cell sorting, hematoxylin and eosin staining and terminal deoxynucleotidyl transferase dUTP nick end labeling indicated neuronal cell loss in the CA1 hippocampus. Rats that had undergone transient global cerebral ischemia for 15 min followed by 1 h of reperfusion exhibited a significant increase in reactive oxygen species (ROS) production in the hippocampus. The I/R-induced ROS overproduction in the hippocampus at 1, 12 and 24 h following I/R was significantly decreased by quercetin pre-treatment. Western blot analysis revealed that the neuroprotective effects of quercetin (5 and 10 mg/kg/day, p.o.) were associated with an upregulation of the I/R-induced suppression of B-cell lymphoma-2 (Bcl-2), Bcl extra large and survivin expression as well as phosphorylation of Bcl-2-associated death promoter. Furthermore, the neuroprotective effects of quercetin (5, 10 mg/kg/day) in the brain were associated with an upregulation of Akt signaling. These findings suggested that the inhibition of I/R-induced brain injury by quercetin likely involves a transcriptional mechanism to enhance anti-apoptotic signaling.

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“…We reported that flavonoids have protective effects on human umbilical vein endothelial (HUVE) cells and rat neuronal cells (PC12) exposed to the highly toxic lipid peroxide linoleic acid hydroperoxide. [9][10][11] In addition, it has been observed that flavonoids suppress the cytotoxicity of hydrogen peroxide toward Chinese hamster cells (V79) 12) and oxidized low-density lipoproteins in human lymphoid cell lines, 13) glucose oxidase-mediated apoptosis in mouse thymocytes, 14) and metal-induced lipid hydroperoxide-dependent lipid peroxidation in a-linoleic acid-loaded rat hepatocytes. 15) On the other hand, it has been suggested that flavonoids act as mutagens, prooxidants, and enzyme inhibitors.…”

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confidence: 99%

Cytotoxicity of Flavonoids toward Cultured Normal Human Cells

Matsuo

Sasaki

Saga

et al. 2005

Biological & Pharmaceutical Bulletin

212

145

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Flavonoids are a group of plant polyphenols and are ubiquitously found in plants. 1,2) Fruits and vegetables, as well as popular beverages such as wine, tea, and coffee, are the main dietary sources of flavonoids. It has been reported that flavonoids show pharmacological effects such as antioxidant, 3,4) antiviral, 5) antitumor, 6) and antiinflammatory activities. 7) In particular, their antioxidant activity has attracted much attention as a possible dietary preventive against cardiovascular and neurodegenerative diseases. 6,8) There are many reports that flavonoids act as antioxidants and protect various cell types from oxidative stress-mediated cell injury. We reported that flavonoids have protective effects on human umbilical vein endothelial (HUVE) cells and rat neuronal cells (PC12) exposed to the highly toxic lipid peroxide linoleic acid hydroperoxide. [9][10][11] In addition, it has been observed that flavonoids suppress the cytotoxicity of hydrogen peroxide toward Chinese hamster cells (V79) 12) and oxidized low-density lipoproteins in human lymphoid cell lines, 13) glucose oxidase-mediated apoptosis in mouse thymocytes, 14) and metal-induced lipid hydroperoxide-dependent lipid peroxidation in a-linoleic acid-loaded rat hepatocytes. 15)On the other hand, it has been suggested that flavonoids act as mutagens, prooxidants, and enzyme inhibitors.16) Further, it has been reported that they exert cytotoxicity at higher concentrations and in the presence of oxidation-catalyzing factors such as transition metal ions. The cytotoxicity of flavonoids toward human promyelocytic leukemia cells (HL-60), 17) human acute myelogeneous leukemia cells (KG1, KG1a, THP-1, and U937), 18) rhesus monkey kidney cells (LLC-MK2), rat glial tumor cells (C6), 19) bovine leukemia virus-transformed lamb embryo kidney fibroblasts (FLK), 20) mouse and hamster pancreatic b-cells (b TC1 and HIT), 21) human fibroblasts (HFK-2), human keratinocytes (HaCaT), human breast cancer adenocarcinoma cells (MCF-7), human neuroblastoma cells (SHEP and WAC-2), and bovine capillary endothelial cells 22) has been found. As shown above, however, there are only a few reports on the cytotoxicity of flavonoids toward human normal cells.If flavonoids are used as dietary factors for health maintenance, relatively large amounts may be ingested. Thus the potentially toxic effects of excessive flavonoid intake must be clarified. 16) In the present paper, we describe the cytotoxicity of nine flavonoids, including the two flavones apigenin and luteolin, the three flavonols 3-hydroxyflavone, kaempherol, and quercetin, the flavonol glycoside rutin, the two flavanones eriodictyol and naringenin, and the flavanol taxifolin (Table 1 and Fig. 1), toward cultured normal human cells, TIG-1 cells and HUVE cells. Further, we examined the intracellular levels of ROS in flavonoid-treated TIG-1 cells using DCF-DA 23) and their incorporation of flavonoids in culture medium. The cytotoxicity of flavonoids, including apigenin, eriodictyol, 3-hydroxyflavone, kaempherol, luteolin,...

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The antioxidant, rather than prooxidant, activities of quercetin on normal cells:quercetin protects mouse thymocytes from glucose oxidase-mediated apoptosis

Cited by 98 publications

References 52 publications

Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pančić and Vis. (Apiaceae)

Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pančić and Vis. (Apiaceae)

Neuroprotective effects of quercetin in a mouse model of brain ischemic/reperfusion injury via anti-apoptotic mechanisms based on the Akt pathway

Cytotoxicity of Flavonoids toward Cultured Normal Human Cells

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