Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pančić and Vis. (Apiaceae)

Abstract

The chemical composition, antimicrobial and antioxidant activity of Eryngium palmatum, an endemic plant species from the Balkan Peninsula, were investigated. The flavonoids apigenin (9.5±0.3 mg g−1) and apigenin 7-O-glucoside (2.4±0.1 mg g−1) were determined in a methanol extract of aerial parts using HPLC analysis. The methanol extract of roots contained catechin (5.0±0.1 mg g−1), epicatechin (2.9±0.1 mg g−1), chlorogenic acid (1.6±0.0 mg g−1), gallic acid (0.9±0.0 mg g−1) and rosmarinic acid (0.9±0.2 mg g−1). GC-FID and GCMS analysis of a chloroform extract of aerial parts showed that the main volatile constituents were falcarinol, linoleic acid, hexadecanoic acid and methyl linoleate (comprising 32.6%; 24.4%; 19.9; 13.2% of the volatile fraction, respectively), while octanoic acid, tetradecanol and dodecanol dominated in the chloroform extract of the roots (34.9%; 25.8%; 22.2% of the volatile fraction, respectively). Investigation of antimicrobial activity by broth microdilution showed that the methanol and chloroform extracts of aerial parts and roots exerted a significant effect (MIC 3.5–15.6 μg mL−1) against tested Gram-positive and Gram-negative bacteria. The methanol extracts of aerial parts or roots exerted moderate ferric reducing antioxidant power, DPPH radical scavenging activity and hydroxyl radical scavenging activity.

In vitro antimicrobial activity of extracts and their fractions from three Eryngium L. species

“…According to other authors, the ethanolic extracts of E. caucaseum and E. bungei have shown moderate antibacterial activity against S. aureus and Streptococcus pyogenes, which cause the most bacterial infections of the skin [24]. Furthermore, the methanolic extracts from aerial parts and roots of E. palmatum showed activity toward S. aureus, P. aeruginosa and C. albicans with MIC values in the range of 7.8-15.6 µg·ml -1 [25]. The antifungal activity (MIC) of the volatile extract of E. duriaei was >20 µg·ml -1 for C. albicans [26].…”

“…The treatment of bacteria and fungi with the fractions gave better results than with extracts. The antimicrobial activity of extracts has been described for some Eryngium species [21][22][23][24][25][26][27][28]. The results of Meot-Duros studies on E. maritimum antimicrobial activity of leaves extracts showed that apolar fractions are generally more active, as compared to polar fraction.…”

Antibacterial and antifungal potentials of the solvents extracts from Eryngium caeruleum, Notholirion thomsonianum and Allium consanguineum

“…In the same way, numerous species of Eryngium have been reported to possess antimicrobial potential. Eryngium palmatum one of medicinally important specie of Eryngium has been verified to be active against microbes [71]. Similarly, the Eryngium genus has also been reported to inhibit the methicillin‐resistant Staphylococcus aureus strains significantly [72].…”

Antioxidant effects of hydroalcoholic and polyphenolic extracts of Peucedanum pastinacifolium Boiss. & Hausskn.

“…Gallic acid possessed the highest FRAPS value, followed by ascorbic acid, BHT, PPE and lastly HAE. In two separate studies, antioxidant properties of three species of ferula and Eryngium palmatum (Umbelliferae) were investigated (2627). Ferric reducing ability of PPE was higher than Ferula Szowitsiana , Ferula hirtella and Ferula oopoda .…”