The Antioxidant Activity of Sterculia stipulata Korth Woods and Leaves by FRAP Method

Abstract

Background: Phenol compounds and flavonoids are known have antioxidant activity. Sterculia genus has secondary metabolite rich of phenols and flavonoids. Objective: The aim of this study of the activity antioxidants of Sterculia stipulata Korth. Woods and leaves by FRAP method. Materials and methods: Extraction done using n-hexane, ethyl acetate, and methanol. The methanol extract was determined antioxidant activity using the FRAP method and also determined the total phenols content, total flavonoids, and phytochemical screening. Results: The antioxidant activity of wood extract was 4.74 ± 1.03 FeEAC (mol/g) while leaves extract 41.17 ± 1.99 FeEAC (mol/g). Total phenols content for wood extract 16.46 ± 3.51 mg GAE/g, for leaves extract 141.62 ± 10.54 mg GAE/g. The total flavonoids content for woods extract was 27.99 ± 0.62 mg QE/g for leaf extract 41.45 ± 5.83 mg QE/g. The compounds of woods and leaves are the same; it is consist of terpenoids, alkaloids, phenols, flavonoids, saponins, terpenoids, and negatives for anthraquinone. Conclusion: The antioxidant activity of the leaves of Sterculia stipulata Korth. is greater than its wood activities.

Analysis of the flavour components, total phenolic content and antioxidant capacity between rice wine from two raw rice and starters

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Microencapsulation of Ruellia tuberosa L. Aqueous Root Extracts Using Chitosan-Sodium Tripolyphosphate and Their In Vitro Biological Activities

“…The FRAP method is used to measure the ability of an antioxidant to reduce or donate electrons to a potassium ferricyanide complex. In this method, antioxidants will act as reducing agents that will reduce Fe 3+ ions from the potassium ferricyanide complex [K 3 Fe(CN) 6 ] to Fe 2+ [ 29 ].…”

“…tuberosa L. extract, and microcapsules. The measurement of antioxidant activity was carried out according to the method reported by [ 29 ]. The assay was initiated by adding 0.5 mL of 0.2 M phosphate buffer pH 6.6 to the samples with various concentrations, 1 to 5 µ g/mL for ascorbic acid, 10–50 µ g/mL for extracts, and 100–500 µ g/mL for microcapsules.…”

Antioxidants, Total Phenolic and Flavonoid Content and Toxicity Assay of Ampelas (Tetracera macrophylla Wall.Ex Hook.F.& Thoms) From Kalimantan-Indonesia

“…The FRAP radical method 10,11 was modified slightly to estimate the antioxidant effect of the samples. The sample concentration was prepared at 1000 ug/mL in methanol, then diluted to 500 ug/mL.…”