The antimicrobial activity of photodynamic therapy against Streptococcus mutans using different photosensitizers

Rolim, Juliana Paiva Marques Lima; Melo, Mary Anne S.; Guedes, Sarah Florindo de Figueiredo; Albuquerque-Filho, Fernando B.; Souza, Jackson R. de; Nogueira, Nádia Accioly Pinto; Zanin, Iriana Carla Junqueira; Rodrigues, Lidiany Karla Azevedo

doi:10.1016/j.jphotobiol.2011.10.001

Cited by 186 publications

(137 citation statements)

References 40 publications

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“…Many studies have reported that AgNPs can damage cell membranes leading to structural changes, which render bacteria more permeable [14,53]. This effect is highly influenced by the nanoparticles' size, shape and concentration [53][54][55][56] and a study using Escherichia coli [14] confirmed that AgNPs accumulation on the membrane cell creates gaps in the integrity of the bilayer which predisposes it to a permeability increase and finally bacterial cell death [19]. Several studies have shown that AgNP activity is strongly dependent on the size [46,47].…”

Section: Salmonella Typhimentioning

confidence: 99%

Silver Nanoparticles as Potential Antibacterial Agents

et al. 2015

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Multi-drug resistance is a growing problem in the treatment of infectious diseases and the widespread use of broad-spectrum antibiotics has produced antibiotic resistance for many human bacterial pathogens. Advances in nanotechnology have opened new horizons in nanomedicine, allowing the synthesis of nanoparticles that can be assembled into complex architectures. Novel studies and technologies are devoted to understanding the mechanisms of disease for the design of new drugs, but unfortunately infectious diseases continue to be a major health burden worldwide. Since ancient times, silver was known for its anti-bacterial effects and for centuries it has been used for prevention and control of disparate infections. Currently nanotechnology and nanomaterials are fully integrated in common applications and objects that we use every day. In addition, the silver nanoparticles are attracting much interest because of their potent antibacterial activity. Many studies have also shown an important activity of silver nanoparticles against bacterial biofilms. This review aims to OPEN ACCESSMolecules 2015, 20 8857 summarize the emerging efforts to address current challenges and solutions in the treatment of infectious diseases, particularly the use of nanosilver antimicrobials.

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Section: Salmonella Typhimentioning

confidence: 99%

Silver Nanoparticles as Potential Antibacterial Agents

et al. 2015

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“…Its possible applications in the management of oral cavity conditions and diseases as an alternative or additional modality for the conventional means and protocols have been confirmed in the late years by numerous studies (24)(25)(26)(27)(28)(29)(30)(31). The area of interests includes: endodontic space disinfection, treatment of primary and secondary dental caries, treatment of periodontal acute and chronic conditions, peri-implantitis.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic inactivation of human dental biofilm Isolated Streptococcus Mutans With 2 Photosensitizers - an In Vitro Study

Doychinova

Kussovski

Tonchev

et al. 2015

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RESULTS:The control samples in dark showed a minor antimicrobial effect. Samples of bacterial suspension + PS + light irradiation expressed a pronounced antibacterial effect on Streptococcus mutans with small differences in the 2 tested PSs. CONCLUSIONS:The clinical strain Streptococcus mutans was susceptible to photodynamic inactivation by the two used photosensitizers: FotoSan® medium and ZnPcMe. Dark toxicity is to be neglected. ZnPcMe should undergo further trials and given the positive photodynamic effect in vitro, to be licensed for clinical use. FotoSan®medium should undergo further in vivo and in vitro experiments in order to establish an alternative protocol for caries prevention.

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“…Moreover, methylene blue produces satisfactory results with remarkable ability to penetrate the polymer matrix of bacterial biofilms due to its hydrophilic nature, low molecular weight cationic nature and water solubility, which allows for greater adhesion to anionic structures, such as teichoic acid [48]. According to Rolim et al [29], the use of methylene blue promotes the formation of oxygen singlet, which is the reactive species responsible for bacterial death, at a rate 1.3 times higher than toluidine blue.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the outer membrane of Gram-negative bacteria is thinner and complex, being formed by a heterogeneous composition of proteins with porin function, lipopolysaccharides, and lipoproteins. Due to the characteristics of their cell walls, Gram-negative bacteria are less permeable to MB reducing their action and therefore are more resistant to PDT than Gram-positive species [25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Enterococcus faecium and Enterococcus faecalis in endodontic infections: antibiotic resistance profile and susceptibility to photodynamic therapy

et al. 2017

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Introduction Enterococcus faecium has become an important microorganism in nosocomial infections with great ability to acquire antibiotic resistance. However, little is known about their presence on the oral cavity. Therefore, our objective was to verify the presence of E. faecium and E. faecalis in endodontic infections and compare their susceptibility to conventional antibiotics and photodynamic therapy. Methods We performed 38 collections from the root canals of different patients. Positive Enterococcus agar samples were submitted to phenotypic and genotypic testing for speciesspecific confirmation. The isolates identified as E. faecium and E. faecalis were tested for susceptibility to antibiotics by the E-test method. After that, the isolates were evaluated for susceptibility to photodynamic therapy (PDT) using methylene blue and gallium arsenide aluminum laser with a wavelength of 660 nm and fluence of 39.5 J/cm 2 (energy of 15 J and time of 428 s).Results Cultures positive for E. faecalis were found in 22 patients (58%). Among these patients, only two had E. faecium in mixed infections with E. faecalis. In the isolates of E. faecalis, 27% were resistant to antibiotics, including tetracycline, ciprofloxacin, and azithromycin. The isolates of E. faecium showed no resistance to the antibiotics tested. Both the isolates of E. faecium and E. faecalis exhibit significant susceptibility to PDT, including the isolates resistant to antibiotics. The reductions achieved by PDT ranged of 2.76 to 4.31 log 10 for E. faecalis strains and of 3.93 to 4.33 log 10 for E. faecium strains. Conclusion E. faecium showed lower prevalence in endodontic infections and higher susceptibility to antibiotics when compared to E. faecalis. In in vitro assays, PDT had a significant antimicrobial activity for both strains.

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The antimicrobial activity of photodynamic therapy against Streptococcus mutans using different photosensitizers

Cited by 186 publications

References 40 publications

Silver Nanoparticles as Potential Antibacterial Agents

Silver Nanoparticles as Potential Antibacterial Agents

Photodynamic inactivation of human dental biofilm Isolated Streptococcus Mutans With 2 Photosensitizers - an In Vitro Study

Enterococcus faecium and Enterococcus faecalis in endodontic infections: antibiotic resistance profile and susceptibility to photodynamic therapy

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