The antigenic components of Erysipelothrix rhusiopathiae

White, Thomas G.; Kalf, George F.

doi:10.1016/0003-9861(61)90176-x

Cited by 14 publications

(5 citation statements)

References 6 publications

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“…The existence of a species-specific proteinaceous 'protective antigen' distinct from type-specific polysaccharide antigens has been repeatedly mentioned by earlier investigators [18,32,42]. A generic antigen extracted from cells of E. rhusiopathiae by acetone and purified by ion exchange chromatography [20,43] was composed of four serologically identical antigens of which one fourth was larger than 200 kDa as measured by analytical centrifugation [44]. White and Verwey [22] examined crude culture supernatants of E. rhusiopathiae and.…”

Section: Discussionmentioning

confidence: 97%

Characterization of a protective protein antigen ofErysipelothrix rhusiopathiae

et al. 1991

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Although vaccination is widely practiced against infection by Erysipelothrix rhusiopathiae in pigs and turkeys, the protective antigen(s) involved have not been fully characterized or purified to homogeneity. Antigens of E. rhusiopathiae strain T28, serotype 2b, and of FRANKFURT XI, serotype N, in culture supernatant and in extracts made with hot acid, 10 mM NaOH, ultrasound or EDTA were compared by SDS-PAGE and immunoblotting and in a mouse protection test. EDTA and 10 mM NaOH yielded highly protective extracts; culture supernatant was less protective and ultrasonic or hot acid extracts stimulated little or no protection in mice. Protective antisera from swine, horses and mice recognized prominent bands of molecular mass (m.m.) of 66-64 and 40-39 kDa in EDTA and 10 mM NaOH extracts. Mice immunized with preparations of the 66-64 kDa band purified by preparative electrophoresis were protected. Both antigens were trypsin sensitive, contained no detectable polysaccharide, and showed a marked tendency to aggregate in the absence of SDS.

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Section: Discussionmentioning

confidence: 97%

Characterization of a protective protein antigen ofErysipelothrix rhusiopathiae

et al. 1991

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“…CE. An acetone-dried powder of E. rhusiopathiae (strain S192) was extracted in 0.05 M aqueous phosphate buffer (pH 7.5) as described previously (14,15).…”

Section: Methodsmentioning

confidence: 99%

“…Serology. Pretreatment, at the time of challenge and terminal blood, was obtained by heart puncture, and the serum was examined for precipitating antibody by agar double diffusion or immunoelectrophoresis, or by both (14).…”

Section: Methodsmentioning

confidence: 99%

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Rabbit Arthritis Induced by Cell-Free Extracts of Erysipelothrix

1971

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A cell-free crude extract of Erysipelothrix rhusiopathiae injected intravenously in rabbits induced pathological lesions of the synovial tissue similar to the lesions seen in rheumatoid arthritis. Fluorescence techniques utilizing tagged specific antiserum and fluorescent-tagged antigen injected into nonsensitized rabbits demonstrated the localization of antigen exclusively in the synovium. Localized concentration of fluorescence was demonstrated as early as 1 hr after injection, before histological changes were apparent. After 48 hr, the fluorescence was concentrated in focal areas and early pathological changes characterized as "mesenchymal activation" were observed. A consideration of the significant role of exogenous antigen in the sensitivity arthritides is discussed. An earlier report from this laboratory showed that a cell-free crude extract (CE) of acetone-dried Erysipelothrix rhusiopathiae could induce a chronic arthritis in rabbits (15). These intraarticular injections produced hyperplasia of the synovial cells and hypertrophy of the vili due to infiltration by lymphocytes and plasma cells which formed aggregates resembling Allison-Ghormley bodies. Identical lesions occurred in the synovium of the untreated knee.

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“…An acetone-dried powder of E. rhusiopathiae strain S192 (kindly supplied by R. D. Shuman, National Animal Disease Laboratory, U.S. Department of Agriculture, Ames, Iowa) was extracted overnight at 4 C in aqueous phosphate buffer (pH 7.5). The preparation of this crude extract was described previously (10). A crude filtrate was passed through HA mem-branes (Millipore Corp., Bedford, Mass.)…”

Section: Methodsmentioning

confidence: 99%

Induction of Experimental Chronic Arthritis in Rabbits by Cell-free Fragments of Erysipelothrix

White

Puls

1969

J Bacteriol

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A cell-free crude extract of Erysipelothrix rhusiopathiae injected by high pressure jet into the knee-joint of rabbits stimulated an acute, mild inflammatory reaction. Additional injections at 3-day intervals induced a chronic condition characterized by hyperplasia of the synovial cells and hypertrophy of the vili, due to infiltration by lymphocytes and plasma cells which formed aggregates resembling Allison-Ghormley bodies. There was also extensive proliferation of stroma vasculature and fibrous tissue. A similar jet injection of the diluent produced an early, transient, acute, and mild inflammation. A mechanism is postulated for fixation of one or more of the chemically characterized antigens in or near the synovium as a means of inducing the localized inflammatory response that predisposes the joint to infection.

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The antigenic components of Erysipelothrix rhusiopathiae

Cited by 14 publications

References 6 publications

Characterization of a protective protein antigen ofErysipelothrix rhusiopathiae

Characterization of a protective protein antigen ofErysipelothrix rhusiopathiae

Rabbit Arthritis Induced by Cell-Free Extracts of Erysipelothrix

Induction of Experimental Chronic Arthritis in Rabbits by Cell-free Fragments of Erysipelothrix

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