Characterization of a protective protein antigen of<i>Erysipelothrix rhusiopathiae</i>

Groschup, Martin H.; Cußler, Klaus; Weiß, R.; Timoney, John F.

doi:10.1017/s0950268800049335

Cited by 24 publications

(20 citation statements)

References 26 publications

(27 reference statements)

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“…The Spa proteins of E. rhusiopathiae are cell membrane-bound [12][13][14][16][17][18] and recognized as the major protective antigen of the species [15]. This present study investigated a wide range of E. rhusiopathiae strains and found that (i) spa-type is not confined to specific serotype groups, especially in those strains isolated from aquatic animals; (ii) a single E. rhusiopathiae strain may possess more than one spa-type; (iii) the degree of cross-protection elicited by conventional erysipelas bacterins is dependent on the Spa-status of the E. rhusiopathiae challenge strain.…”

Section: Discussionmentioning

confidence: 99%

“…The 64-66 kilodalton (kDa) cell surface protein of E. rhusiopathiae is responsible for eliciting highly protective antibodies [12][13][14], and is considered to be the major immunogenic antigen of the specie [15]. The gene encoding the protective 64-66 kDa protein was first sequenced by Makino et al [16] who named it, surface protective antigen A (spaA).…”

Section: Introductionmentioning

confidence: 99%

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Erysipelothrix rhusiopathiae: Association of Spa-type with serotype and role in protective immunity

Ingebritson

Roth

Hauer

2010

Vaccine

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A collection of swine, fish, and cetacean Erysipelothrix rhusiopathiae strains representing 16 serotypes was analyzed for possession of the three currently recognized surface protective antigen (spa)-types: spaA, spaB, and spaC. Polymerase chain reaction (PCR) assays and Western blotting with a SpaA-specific monoclonal antibody demonstrated that spa-type is not confined to specific serotype groups. In particular, the spa-type of strains of aquatic origin was more variable than those of terrestrial origin, and possessed the distinct ability to express more than one spa. In a cross-protection study, mice immunized with an E. rhusiopathiae serotype 2 SpaA-type strain and challenged with various E. rhusiopathiae isolates were completely protected against strains exhibiting a single homologous spa, but variably protected against strains possessing a heterologous spa or those harboring more than one spa-type. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. Keywords: Erysipelothrix rhusiopathiae Surface protective antigen Erysipelas a b s t r a c t A collection of swine, fish, and cetacean Erysipelothrix rhusiopathiae strains representing 16 serotypes was analyzed for possession of the three currently recognized surface protective antigen (spa)-types: spaA, spaB, and spaC. Polymerase chain reaction (PCR) assays and Western blotting with a SpaA-specific monoclonal antibody demonstrated that spa-type is not confined to specific serotype groups. In particular, the spa-type of strains of aquatic origin was more variable than those of terrestrial origin, and possessed the distinct ability to express more than one spa. In a cross-protection study, mice immunized with an E. rhusiopathiae serotype 2 SpaA-type strain and challenged with various E. rhusiopathiae isolates were completely protected against strains exhibiting a single homologous spa, but variably protected against strains possessing a heterologous spa or those harboring more than one spa-type.Published by Elsevier Ltd.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Erysipelothrix rhusiopathiae: Association of Spa-type with serotype and role in protective immunity

Ingebritson

Roth

Hauer

2010

Vaccine

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“…The major protective antigen of E. rhusiopathiae is the socalled 64-to 66-kDa antigen (1,6,7,13,19). Makino et al (14) cloned the gene encoding the 69.9-kDa protective antigen of strain Tama of serotype 2 and named it the protein surface protective antigen (SpaA).…”

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confidence: 99%

Enzyme-Linked Immunosorbent Assay Employing a Recombinant Antigen for Detection of Protective Antibody against Swine Erysipelas

et al. 2003

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The specificities and sensitivities of five recombinant proteins of the surface protective antigen (SpaA) of Erysipelothrix rhusiopathiae were examined by indirect enzyme-linked immunosorbent assay (ELISA) with the aim of developing a reliable serological test for the detection of protective antibody against E. rhusiopathiae. Fully mature protein and the N-terminal 416 amino acids (SpaA416) showed sufficient antigenicities, and further examination was done with SpaA416 because of its higher yield. The antibody titers of pigs experimentally immunized with commercial live vaccine and two types of inactivated vaccines clearly increased after immunization, and all pigs were completely protected against challenge with virulent strains. On the other hand, the antibody titers of nonimmunized control pigs remained very low until they were challenged, and all showed severe symptoms or subsequently died. Interference with the production of antibody against live vaccine by maternal antibody or porcine respiratory and reproductive syndrome virus infection 1 week after vaccination was also clearly detected. Because the ELISA titer correlated well with the protection results, the specificity and sensitivity of the ELISA were further evaluated with sera collected from pigs reared on 1 farm on which animals had acute septicemia, 2 farms on which the animals were infected or free from infection, and 10 farms on which the animals were vaccinated with live vaccine, among others. The ELISA titers clearly revealed the conditions of the herds. These results indicate that the SpaA416 ELISA is an effective method not only for evaluating pigs for the presence of protective antibody levels resulting from vaccination or maternal antibody but also for detecting antibody produced by natural infection. This test has important potential for the effective control of swine erysipelas.

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“…Lachmann and Deicher [4] reported that major proteins with molecular weights of 78, 72, 68 and 48 kDa were obtained in EDTA, CHAPS, TritonX, and SDS extracts of E. rhusiopathiae T28. Groschup et al [2] found that a 66-64 kDa antigen prepared by extraction of E. rhusiopathiae with 10 mM NaOH had a protective effect in mice. It is therefore considered that one of the protective antigens was the 66-64 kDa protein released from the cell surface of E. rhusiopathiae.…”

Section: Discussionmentioning

confidence: 99%

“…Groschup et al [2] reported that 10 mM NaOH extracts of E. rhusiopathiae were protective in mice. Kobayashi et al [3] reported that culture filtrate and alkaline-extracted antigens from cells of the attenuated strain Koganei of E. rhusiopathiae induced protective immunity in mice.…”

Section: Bacterial Strainsmentioning

confidence: 99%

Protective Effect of NaOH-Extracted Erysipelothrix rhusiopathiae Vaccine in Pigs.

Kitajima

Oishi

Amimoto

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. A vaccine was prepared from a NaOH-extracted antigen of the Kyoto strain (serovar 2) of Erysipelothrix rhusiopathiae (E. rhusiopathiae) with an oil adjuvant, and was injected twice at 3-week intervals into SPF pigs and conventional pigs with maternal antibodies. After the second vaccination, IgG-GA titers of immunized SPF pigs were more than 256-fold at 3 weeks, and immunized pigs with maternal antibodies were 64-fold at 7 weeks. The pig with maternal antibodies vaccinated once with live vaccine had less than 4-fold titers. The ELISA antibody titers which were measured by using the NaOH-extracted antigen showed similar transition to the IgG-GA antibody titers. All immunized pigs and nonvaccinated control pigs were challenged with the strains Fujisawa (serovar 1a) or Saitama-1 (serovar 2). After challenge exposure, all pigs immunized with the NaOH-extracted vaccine showed no clinical signs and survived, and the pig immunized with the live vaccine had a local rhomboidal lesion at the site of the injection. Nonvaccinated pigs developed typical symptoms of E. rhusiopathiae infection and one of them died. After the autopsy, the challenge strains were not recovered from the main organs except tonsils of the pigs immunized with the NaOH-extracted vaccine. These results indicated that the NaOH-extracted vaccine induces a protective effect in pigs with maternal antibodies as well as in SPF pigs negative for such antibodies, and that 67-64, 62-60 kDa proteins in the NaOH-extracted antigen play an important role in protecting against E. rhusiopathiae infection. -KEY WORDS: Erysipelothrix rhusiopathiae, NaOH-extracted antigen, vaccine.

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Characterization of a protective protein antigen ofErysipelothrix rhusiopathiae

Cited by 24 publications

References 26 publications

Erysipelothrix rhusiopathiae: Association of Spa-type with serotype and role in protective immunity

Erysipelothrix rhusiopathiae: Association of Spa-type with serotype and role in protective immunity

Enzyme-Linked Immunosorbent Assay Employing a Recombinant Antigen for Detection of Protective Antibody against Swine Erysipelas

Protective Effect of NaOH-Extracted Erysipelothrix rhusiopathiae Vaccine in Pigs.

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