Induction of Experimental Chronic Arthritis in Rabbits by Cell-free Fragments of
            <i>Erysipelothrix</i>

White, Thomas G.; Puls, Jerry L.

doi:10.1128/jb.98.2.403-406.1969

Cited by 14 publications

(5 citation statements)

References 8 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The broad objectives of our studies are etiologic in nature. We have demonstrated the capacity of this extract to induce advanced, progressive arthritis in rabbits (15). The critical event in this progressive disease is the initial change induced in the synovium; its chronicity is probably the result of many factors with the essential ingredient being persistence of the antigen.…”

Section: Resultsmentioning

confidence: 90%

“…CE. An acetone-dried powder of E. rhusiopathiae (strain S192) was extracted in 0.05 M aqueous phosphate buffer (pH 7.5) as described previously (14,15).…”

Section: Methodsmentioning

confidence: 99%

“…To repeat, our concept does not preclude antigens from other microorganisms as elictors of this reaction; Erysipelothrix was used in the model because of its known ability to induce arthritis in its natural host, swine, and because of the knowledge of its biochemical properties (5,14,15).…”

Section: Figmentioning

confidence: 99%

See 2 more Smart Citations

Rabbit Arthritis Induced by Cell-Free Extracts of Erysipelothrix

1971

Self Cite

View full text Add to dashboard Cite

A cell-free crude extract of Erysipelothrix rhusiopathiae injected intravenously in rabbits induced pathological lesions of the synovial tissue similar to the lesions seen in rheumatoid arthritis. Fluorescence techniques utilizing tagged specific antiserum and fluorescent-tagged antigen injected into nonsensitized rabbits demonstrated the localization of antigen exclusively in the synovium. Localized concentration of fluorescence was demonstrated as early as 1 hr after injection, before histological changes were apparent. After 48 hr, the fluorescence was concentrated in focal areas and early pathological changes characterized as "mesenchymal activation" were observed. A consideration of the significant role of exogenous antigen in the sensitivity arthritides is discussed. An earlier report from this laboratory showed that a cell-free crude extract (CE) of acetone-dried Erysipelothrix rhusiopathiae could induce a chronic arthritis in rabbits (15). These intraarticular injections produced hyperplasia of the synovial cells and hypertrophy of the vili due to infiltration by lymphocytes and plasma cells which formed aggregates resembling Allison-Ghormley bodies. Identical lesions occurred in the synovium of the untreated knee.

show abstract

Section: Resultsmentioning

confidence: 90%

“…CE. An acetone-dried powder of E. rhusiopathiae (strain S192) was extracted in 0.05 M aqueous phosphate buffer (pH 7.5) as described previously (14,15).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Rabbit Arthritis Induced by Cell-Free Extracts of Erysipelothrix

1971

Self Cite

View full text Add to dashboard Cite

show abstract

“…In swine an acute infection with E. rhusiopathiae results in a chronic destructive arthritis which resembles, in many respects, human rheumatoid arthritis (43,49,50). After adaption into rats (51), an experimental model became available that enabled us to follow a sequence of events that may lead to chronic arthritis.…”

Section: Discussionmentioning

confidence: 99%

“…Arthritis may also develop after infection with bacteria (1,5,20,24,38). Among arthritisinducing bacteria, gram-positive Erysipelothrix rhusiopathiae is a natural pathogen of swine and initially induces an acute infection that rapidly generalizes into a septicemic phase with high fever and weight loss, which is then followed by a self-sustaining, chronic arthritis (43,49,50). Since E. rhusiopathiae produces a similar disease in rats (51), it represents a convenient model to study the sequence of events that lead from infection to arthritis.…”

mentioning

confidence: 99%

Activation of macrophages in an experimental rat model of arthritis induced by Erysipelothrix rhusiopathiae infection

et al. 1989

View full text Add to dashboard Cite

Infection of Lewis rats with Erysipelothrix rhusiopathiae represents an experimental model system of acute and chronic arthritis. We studied here the acute inflammatory phase with respect to stimulation of macrophages and lymphocytes. Intragluteal injection of viable E. rhusiopathiae (102 to 104 bacteria) rapidly induced generalized inflammation, loss of body weight, hind leg arthritis, and systemic macrophage activation within 2 to 3 days. The same symptoms could also be evoked by injection of dead E. rhusiopathiae. Ex vivo, peritoneal macrophages released large amounts of tumor necrosis factor alpha on day 2 and interleukin-1 on day 3, whereas production of prostaglandin E2 was delayed to days 5 to 7 and appeared to counteract tumor necrosis factor alpha synthesis. The inflammatory response and development of arthritis were strongly dependent on T lymphocytes, as evidenced by the following findings: (i) lymphocytes released lymphokines that activated macrophages to enhanced mediator release; (ii) treatment of rats with cyclosporin A reduced infection-induced macrophage activation; (iii) mitogen-stimulated thymocyte proliferation was enhanced, indicating an infection-induced maturation-differentiation process in the thymus; and (iv) in T-cell-deficient nude rats, a higher dose of bacteria was required for infection, the inflammatory response was less severe, and only mild, but not chronic, arthritis developed. Thus, an E. rhusiopathiae-induced inflammation in rats provides a useful tool to characterize activated macrophages and T lymphocytes during the development of acute arthritis and its transition into the chronic form.

show abstract

Specific detection and semiquantitation of microorganisms in tissue by nucleic acid hybridization. II. Investigation of synovia from pigs with chronic erysipelothrix arthritis

Steinman

Hsu

1976

Arthritis & Rheumatism

View full text Add to dashboard Cite

Synovial tissues from animals with chronic Erysipelothrix arthritis were examined by a technique based on RNA-DNA hybridization in an attempt to detect the inducing organism, Erysipelothrix insidiosa (EI). Twelve synovial specimens from 5 animals whose joints lacked culturable EI also lacked EI detectable by this technique. Because the technique is capable of detecting approximately one organism per 50 mammalian cells, it is comcluded that no more than this number were present in the involved tissue. Implications of these results for proposed pathogenetic mechanisms in this disorder and in human rheumatoid arthritis are discussed.In its chronic stage porcine Erysipelothrix arritis shares many features with human rheumatoid arthritis (1). The most notable difference is that a known bacterium, Erysipelothrix insidiosa (EI), is required to induce the animal disorder. But, as with rheumatoid

show abstract

Induction of Experimental Chronic Arthritis in Rabbits by Cell-free Fragments of Erysipelothrix

Cited by 14 publications

References 8 publications

Rabbit Arthritis Induced by Cell-Free Extracts of Erysipelothrix

Rabbit Arthritis Induced by Cell-Free Extracts of Erysipelothrix

Activation of macrophages in an experimental rat model of arthritis induced by Erysipelothrix rhusiopathiae infection

Specific detection and semiquantitation of microorganisms in tissue by nucleic acid hybridization. II. Investigation of synovia from pigs with chronic erysipelothrix arthritis

Contact Info

Product

Resources

About