The Antibacterial Efficacy of Biopure MTAD in Root Canal Contaminated with Enterococcus faecalis

Abstract

Aim. The purpose of this in vitro study was to assess the antimicrobial efficacy of Biopure MTAD against E. faecalis in contaminated root canals. Materials and Methods. Forty-two single rooted extracted human teeth were inoculated with E. faecalis and incubated for four weeks. The samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (NaOCl); 3% NaOCl; BioPure MTAD; 1.5% NaOCl/17% EDTA; or 3% NaOCl/17% EDTA. After a one-week incubation, complete disinfection was confirmed by the absence of turbidity in the incubation media. Dentin shavings were taken from samples with no turbidity to verify whether E. faecalis was present in dentin tubules. Results were analyzed statistically using Fisher's exact test, with the level of significance set at P < 0.05. Results. Statistical analysis of the data obtained at Day 7 and after dentin shaving analysis showed that BioPure MTAD had significantly greater antibacterial activity than 1.5% NaOCl, 1.5% NaOCl/17% EDTA and 3% NaOCl/17% EDTA. No significant difference was detected between MTAD and 3% NaOCl. Conclusions. These findings suggest that BioPure MTAD possesses superior bactericidal activity compared with NaOCl and EDTA against E. faecalis.

The Comparative Evaluation of the Post-Antimicrobial Effect of MTAD® and 2% Chlorhexidine against Enterococcus faecalis of Permanent Teeth with Necrotic Pulp

“…Microbiological samples were taken from patients with permanent teeth with necrotic pulp, as E. faecalis is the most persistent type of bacteria in necrotic teeth, as stated by Kamberi et al, [3].…”

“…That's why it is considered one of the most resistant bacteria in necrotic teeth, and its persistence causes the failure of the root canal treatment. And it requires different visits and using intracanal medications in-between visits to eradicate this bacteria from the root canal, So it is very important to find an irrigating solution which has a strong bactericidal effect of getting rid of bacteria and improve the success rate of root canal treatment of necrotic teeth [3].…”

Comparative antimicrobial efficacy of selected root canal irrigants on commonly isolated microorganisms in endodontic infection

“…In contrast to the above results, few studies reveal no effect of metronidazole against P. acnes . [24]…”

New Intracanal Formulations Containing Doxycycline or Chlorhexidine Against Enterococcus faecalis

“…15 A new irrigant solution, MTAD, 13 containing a mixture of 3% doxycycline, 4, 25% citric acid and a detergent (0.5% Polysorbate 80) 16 was also introduced and some studies suggest that MTAD has a potent antibacterial effect against E. faecalis. 17,18 MTAD presents an acidic condition (ph = 2.15), which could explain the changes on the structure of the dentinal tubules, helping its diffusion through them. 19 After these considerations, the aim of this study was to create an innovative intracanal dressing gels based on the formulation of MTAD containing doxycycline, along with another one containing chlorhexidine, to compare their ability to eliminate E. faecalis from root canals.…”