The Comparative Evaluation of the Post-Antimicrobial Effect of MTAD® and 2% Chlorhexidine against Enterococcus faecalis of Permanent Teeth with Necrotic Pulp

Abstract

AIM: Enterococcus faecalis is one of the most resistant bacteria in necrotic teeth. That’s why the goal of this study was to determine the post-antibiotic effect of MTAD®& 2% Chlorhexidine® as root canal irrigating solution on clinical isolates of E. faecalis from infected root canals of permanent teeth, using the spectrophotometric technique.
 MATERIAL AND METHODS: The antibacterial efficacy of Chloramphenicol 30 mcg, Nitrofurantoin 300 mcg, Vancomycin 5 mcg, Amoxicillin/clavulanic acid 30 mcg and Ofloxacin 5 mcg against E. faecalis was compared using the Disc diffusion method. Patients were selected for this study with permanent necrotic teeth. The sterile paper point was inserted inside the infected root canal and left for 60 seconds; to obtain the microbiological sample. Postantibiotic effect of MTAD® and 2% Chlorohexidine® on E. faecalis was compared. The absorbance of bacterial growth was examined for both irrigating solutions during the first 10 hours with an hour interval, and then tested at 48, 72, 96 up to 240 hours.
 RESULTS: The results showed that during the first 10 hours, MTAD® showed immediate antibacterial effect and maintained its higher antibacterial activity than 2% chlorohexidine®. After 48, 72, 96 and 240 hours, both MTAD® and 2% chlorohexidine® showed the same prolonged action of post-antibiotic effect against E. faecalis with a non-significant difference. According to Antibiotic sensitivity, the results revealed MTAD® is the most effective antimicrobial drug, showing the highest zone of inhibition, followed by 2% Chlorhexidine and Nitrofurantoin 300 mcg which showed the same inhibitory activity
 CONCLUSION: From the current study, it can be concluded that MTAD® has a strong bactericidal effect against E. faecalis and showed the highest zone of inhibition.

Effect of Addition of Cetylpyridinium Chloride Cationic Surfactant on the Antimicrobial Activity of Chlorhexidine Endodontic Irrigant

“…The antimicrobial effect of MTAD may be primarily attributed to the doxycycline component of the irrigant, a broad-spectrum antibiotic effective against a wide range of microorganisms. Doxycycline inhibits protein synthesis and reversibly binds to the 30s ribosomal subunits of susceptible microorganisms [ 18 ].…”

The Antibacterial Effect of Cysteamine and Its Combinations With Various Intracanal Medications Against Enterococcus Faecalis

“…Scrapping the dentin surface was done to have samples from deeper dentin levels. Paper points were used to absorb the transport fluid with dentin chips formed (15,29,30) .…”

Comparison of antimicrobial efficacy of Calcipex and Metapex in endodontic treatment of chronic apical periodontitis: a randomised controlled trial study protocol

“…After completion of the preparation, the canals will be irrigated with saline and dried with paper points. A sterile paper point (size 20; EZ Endo, USA) will be inserted to the confirmed working length of the canal, and retained in position for 60 s for sampling 18 (S1) to soak all the canal contents. Afterwards, the paper point will be gently removed and placed in an Eppendorf tube (5 mL) containing Brain Heart Infusion Broth and sent directly to the microbiology laboratory for microbial cultivation.…”

“… 17 On the contrary, Estrela et al 10 examined the antimicrobial efficacy of CH with certain vehicles and concluded that the vehicles did not influence the antimicrobial activity of CH. 18 Estrela et al 10 in another study concluded that addition of iodoform does not improve the antimicrobial efficacy of CH-saline solution. Whether CH with iodoform is more effective than CH alone against endodontic pathogens is yet to be established.…”