The Antibacterial Effect of Cysteamine and Its Combinations With Various Intracanal Medications Against Enterococcus Faecalis

Abstract

Background:The microbiological ecosystem is considered the main obstacle in endodontics. Therefore, canal disinfection became a great priority. Aim of the study:This study was established to assess cysteamine's antibacterial effect against E. Faecalis and compare the improvement effect to its combination with various intracanal medicaments. Methods and Materials:Cleaning and shaping were performed on human single-rooted teeth then inoculated by E. faecalis biofilm. Samples were divided randomly into three groups: Group I to verify the maturation of E. faecalis biofilm inside the root canal using SEM. Group II were divided into four subgroups according to the intracanal medication used. Subgroup A was treated with Cysteamine. Subgroup B was treated with Chlorhexidine Cysteamine combination. Subgroup C was treated with Calcium hydroxide (CaOH) Cysteamine combination. Subgroup D was treated with Triple antibiotic paste Cysteamine combination. Group III were divided into two subgroups treated with plain gel: positive control and negative control. Samples collected from root canals were used for viable count (CFU/ml). Log transformation of the data was carried out. The statistical significance level was set at P < 0.05. Results:There was a significant difference between different groups (p<0.001). Post hoc pairwise comparisons showed statistically significant difference between subgroups except for the CaOH Cysteamine combination subgroup and TAP Cysteamine combination subgroup. Conclusion:Cysteamine usage provides a synergistic antibacterial effect with other intracanal medications. CHX Cysteamine combination was the most effective antibacterial drug that provided effectiveness against E. faecalis.

The Cytotoxic Effect of Cysteamine and its Combinations with Various Endodontic Intracanal Medications on Fibroblast Cells; in Vitro Study

“…Results of both cell viability %, which re ected the number of lived/metabolically active cells in a population, and IC50 which re ected the dose of the cytotoxic compound at which 50% viability on BHK broblast cells was achieved were in harmony as they showed that TAP Cysteamine combination had the lowest cytotoxicity level compared to other intracanal combinations followed by Cysteamine and the highest cytotoxicity was with Chlorhexidine Cysteamine combination and usage of CHX Cysteamine combination should be in lowest concentrations as described by Mirhadi et al (32) It was approved that cysteamine has an acceptable antibacterial effect and a good synergistic effect with other intracanal medications, especially with triple antibiotic paste which showed a higher antibacterial effect (7,37) . Our study found that the TAP Cysteamine combination the least cytotoxic effect compared to others.…”