The antiapoptotic and antioxidant effects of eugenol against cisplatin‐induced testicular damage in the experimental model

Abstract

Testicular dysfunction or damage is among the critical side effects of chemotherapeutic drugs like cisplatin. This study was mapped out to assess the possible therapeutic effect of eugenol on cisplatin‐induced testicular damage. In this experimental study, a single dose of cisplatin (15 mg/kg) was given intraperitoneally. After 72 hr of cisplatin injection, rats were sacrificed and testis tissues were removed. Tissues were examined by biochemical, histopathological and immunohistochemical methods. While tissue lipid peroxidation product and apoptotic marker levels increased, antioxidant enzyme activities of testis tissue were decreased in the cisplatin group. Additionally, histopathological damage was also determined in testis tissue. Contrary to all these results, the severity of damage in the tissue was reduced histopathologically owing to eugenol treatment. The lipid peroxidation decreased and antioxidant enzyme activities increased in the eugenol treatment group. It has been determined that eugenol has a therapeutic effect on oxidative stress and apoptosis against cisplatin‐induced testicular damage.

Protective Effects of Nucleosides‐Rich Extract from Cordyceps cicadae against Cisplatin Induced Testicular Damage.

“…A single dose of CISP (10 mg/kg, dissolved in 5 % DMSO/saline) was administered by intraperitoneal injection to the rats in the CISP and CISP+NRCE groups. The dose of CISP and NRCE were chosen based on previous literature [11,33] . After the last day of treatment, the rats were euthanized with sodium thiopental (150 mg/kg) and blood samples were obtained through cardiac puncture, centrifuged (3000 rpm for 15 min at 4 °C) to obtain the sera, which was subsequently used for the determination of testosterone and follicle stimulating hormone (FSH) levels.…”

“…The dose of CISP and NRCE were chosen based on previous literature. [11,33] After the last day of treatment, the rats were euthanized with sodium thiopental (150 mg/kg) and blood samples were obtained through cardiac puncture, centrifuged (3000 rpm for 15 min at 4°C) to obtain the sera, which was subsequently used for the determination of testosterone and follicle stimulating hormone (FSH) levels. The testes were rapidly excised, rinsed with normal saline and weighed.…”

Eugenol Attenuates Cerebral Ischemia-Reperfusion Injury by Enhancing Autophagy via AMPK-mTOR-P70S6K Pathway

“…Eugenol is one of active compounds of a well-known traditional Chinese medicine Acorus gramineus, which has attracted wide attention for its antiinflammatory (El-Kady et al, 2019), antiapoptotic (Ekinci Akdemir et al, 2019), antioxidant (Ekinci Akdemir et al, 2019), and antitumor (Fangjun and Zhijia, 2018) effects. A previous study showed that eugenol could protect against hepatic ischemia-reperfusion injury (Abd El Motteleb et al, 2014).…”

Ocimum tenuiflorum mitigates iron‐induced testicular toxicity via modulation of redox imbalance, cholinergic and purinergic dysfunctions, and glucose metabolizing enzymes activities

“…Testicular oxidative stress has been reported to be detrimental to male fertility health (Turner & Lysiak, 2008; Yesil et al., 2018). The use of exogenous antioxidants such as eugenol (Table 1) have been reported to improve testicular dysfunctions (Aitken & Roman, 2009; Ekinci akdemir et al., 2019; Imam et al., 2017). The ability of the infusion to restore the testicular antioxidant capacity indicates a potent protective effect against oxidative testicular injury, which may be attributed to its phenolic content.…”