The ameliorative role of specific probiotic combinations on bone loss in the ovariectomized rat model

Abstract

Background Osteoporosis, a skeletal disease described by impaired bone strength, cause an increased risk of fractures. We aimed in this study to clarify which particular wise combination of probiotics has the most beneficial effect in the rat model of osteoporosis. Methods Sixty-three mature female Sprague Dawley rats (12–14 weeks old, weight 200 ± 20 g) were ovariectomized and then divided into nine random groups, each group consisting of 7 rats. Lactic acid bacteria were isolated from traditional fermented yogurt on the northern coast of the Persian Gulf. Seven combinations of probiotics, each containing three probiotic strains, were designed and administered (1 × 10 9 CFU / ml/strain daily along with their water) to treat ovariectomized rats. The period from ovariectomy to eutanásia was 3 months. For evaluating femur, spine, and tibia, bone mineral density (BMD), and bone mineral content (BMC), Dual-energy X-ray absorptiometry (DEXA) scans were performed. Also, effect of probiotic combinations was assessed on biochemical markers including vitamin D, calcium, phosphorus, and alkaline phosphatase in serum. Results Combination NO 4, containing L. acidophilus, B. longum, and L. reuteri, is the most influential group on global, spine, and femur BMD. Combination NO 3, containing L. acidophilus , L. casei , and L. reuteri, also significantly affects the BMD of the tibia among the treatment group. We found that the combination NO 4 had the most significant ameliorative effect on global BMC. Also, combination NO 1 (comprising L. acidophilus, L. casei, and B. longum) , NO 6 (containing L. casei, B. longum, and Bacillus coagulans), NO 7 (containing L. casei, L. reuteri, and B. longum), and NO 4 had the most considerable raising effect on spine BMC. In addition, the serum calcium and Vitamin D concentration in the groups NO 4, 6, and 7 were significantly higher than in OVX groups, whereas the alkaline phosphatase concentration was considerably reduced in these groups. Conclusion Among nine effective probiotics, a combination containing L. acidophilus, B. longum, and L. reuteri is the most influential group in ovariectomized osteoporotic rat.

The Potential Value of Probiotics after Dental Implant Placement

“…Moreover, research on probiotics may be more important not only for healthy patients (animals), but also for patients with certain underlying diseases and elderly individuals. Current evidence suggests that probiotics exhibit significant potential in mitigating the impact of underlying diseases, particularly in the context of diabetes and osteoporosis [ 64 , 67 , 133 ]. In the field of oral implantology, conditions such as diabetes and osteoporosis are generally recognized as high risks for implant health [ 7 , 9 , 10 ].…”

“…It has been suggested in the literature that supplementation with specific strains of Bifidobacterium , particularly in the elderly population, can expedite fracture repair [ 62 ]. In models of osteoporosis induced by various factors, administration of probiotics such as Lactobacillaceae and Bifidobacterium has been shown to increase bone density, attenuate bone loss, and maintain bone homeostasis [ 56 , 61 , 63 , 64 , 65 , 66 ]. In clinical research, the effects of probiotic supplementation have been comparable to those of vitamin D and calcium supplementation, and it has been suggested as one of the therapeutic approaches for osteoporosis patients [ 67 , 68 ].…”

“…In addition, probiotics that promote the digestion and absorption of nutrients can also contribute to the health of the skeletal system [ 55 ]. Various probiotics, such as Lactobacillus acidophilus , Lacticaseibacillus casei , Limosilactobacillus reuteri and Bifidobacterium longum have been shown to enhance the absorption of calcium, vitamin D, and vitamin K [ 64 , 117 , 118 , 119 ]. These nutrients are considered crucial for implant osseointegration and stability of the surrounding bone tissue [ 120 , 121 , 122 , 123 , 124 ].…”

Effects of Lactobacillus acidophilus and L. reuteri on bone mass and gut microbiota in ovariectomized mice

“…Probiotics include xylooligosaccharides (XOS), fructooligosaccharides(FOS), glucomannans, and acid-hydrolyzed high amylose corn starch (AH-HAS) (26). Bone loss in ovariectomized rat models can be ameliorated by single or multiple probiotic feeding combinations (27). Prebiotics mixed with probiotics or fermented can also be used to enhance calcium absorption and anti-osteoporosis effect (28).…”

The ameliorating effect of limosilactobacillus fermentum and its supernatant postbiotic on cisplatin-induced chronic kidney disease in an animal model

“…A P-value less than 0.05 was considered statistically significant. Data were expressed as means ± Standard Deviation (n = 5) [ 45 ].…”