The adaptive response of bacterial food-borne pathogens in the environment, host and food: Implications for food safety

Álvarez‐Ordóñez, Avelino; Broussolle, Véronique; Colin, P.; Nguyen-Thé, Christophe; Prieto, Miguel

doi:10.1016/j.ijfoodmicro.2015.06.004

Cited by 101 publications

(57 citation statements)

References 116 publications

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“…Polyphasic approaches are able to reduce the bias posed by a single method with too much emphasis on only phenotypic, chemotaxonomic or genotypic characteristics. Moreover, there is high level of agreement between the polyphasic arrangement obtained and the classification recently made based on the multivariate analysis of stress resistance patterns (Alvarez-Ordóñez et al, 2015), which shows that this approach is able to not only reveal taxonomic relationships but also provide useful cues on strain ecology and physiology, giving information on strain behaviour in relation to food and the environment. To improve the taxonomic significance of strain arrangements, additional genotyping techniques such as multilocus sequence typing (MLST) could be integrated in the polyphasic approach.…”

Section: Polyphasic Analysis and Integration Of Resultsmentioning

confidence: 55%

Comparison of methods for the identification and sub-typing of O157 and non-O157 Escherichia coli serotypes and their integration into a polyphasic taxonomy approach

Prieto-Calvo¹,

Omer²,

Alvseike³

et al. 2016

Irish Journal of Agricultural and Food Research

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Phenotypic, chemotaxonomic and genotypic data from 12 strains of Escherichia coli were collected, including carbon source utilisation profiles, ribotypes, sequencing data of the 16S-23S rRNA internal transcribed region (ITS) and Fourier transform-infrared (FT-IR) spectroscopic profiles. The objectives were to compare several identification systems for E. coli and to develop and test a polyphasic taxonomic approach using the four methodologies combined for the subtyping of O157 and non-O157 E. coli. The nucleotide sequences of the 16S-23S rRNA ITS regions were amplified by polymerase chain reaction (PCR), sequenced and compared with reference data available at the GenBank database using the Basic Local Alignment Search Tool (BLAST) . Additional information comprising the utilisation of carbon sources, riboprint profiles and FT-IR spectra was also collected. The capacity of the methods for the identification and typing of E. coli to species and subspecies levels was evaluated. Data were transformed and integrated to present polyphasic hierarchical clusters and relationships. The study reports the use of an integrated scheme comprising phenotypic, chemotaxonomic and genotypic information (carbon source profile, sequencing of the 16S-23S rRNA ITS, ribotyping and FT-IR spectroscopy) for a more precise characterisation and identification of E. coli. The results showed that identification of E. coli strains by each individual method was limited mainly by the extension and quality of reference databases. On the contrary, the polyphasic approach, whereby heterogeneous taxonomic data were combined and weighted, improved the identification results, gave more consistency to the final clustering and provided additional information on the taxonomic structure and phenotypic behaviour of strains, as shown by the close clustering of strains with similar stress resistance patterns.

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Section: Polyphasic Analysis and Integration Of Resultsmentioning

confidence: 55%

Comparison of methods for the identification and sub-typing of O157 and non-O157 Escherichia coli serotypes and their integration into a polyphasic taxonomy approach

Prieto-Calvo¹,

Omer²,

Alvseike³

et al. 2016

Irish Journal of Agricultural and Food Research

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“…On the other hand, bacteria can also respond to adverse conditions in a transient way, through so-called stress tolerance responses. Bacterial stress tolerance responses include structural and physiological modifications in the cell, and complex genetic regulatory machines mediate them [41].…”

Section: Discussionmentioning

confidence: 99%

Larvicidal evaluation of the Origanum majorana L. Essential Oil against the larvae of the Aedes aegypti mosquito

Chaves

Martins

Rodrigues

et al. 2019

Preprint

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This study evaluated the larvicidal activity of O. majorana essential oil, identified the chemical composition, evaluated the antimicrobial, cytotoxic and antioxidant potential. The larvicidal activity was evaluated against larvae of the third stage of Aedes aegypti, whereas the chemical composition was identified by gas chromatography coupled to mass spectrometer, the antimicrobial activity was carried out against the bacteria Pseudomonas aeruginosa, Escherichia coli and Staphylococcus auereus, the antioxidant activity was evaluated from of 2.2-diphenyl-1-picryl-hydrazila sequestration and Artemia salina cytotoxicity. Regarding to the results, the larvicidal activity showed that O. majorana essential oil caused high mortality in A. aegypti larvae. In the chromatographic analysis, the main component found in O. majorana essential oil was pulegone (57.05%), followed by the other components verbenone (16.92%), trans-p-menthan-2-one (8.57%), iso-menthone (5.58%), piperitone (2.83%), 3-octanol (2.35%) and isopulegol (1.47%). The antimicrobial activity showed that E. coli and P. aeruginosa bacteria were more sensitive to oil than S. aureus, which was resistant at all concentrations. Essential oil did not present antioxidant activity, but it has high cytotoxic activity against A. salina.

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“…However the potential metabolic capacities of pathogens to adapt to food and host environments would worthy to be investigated [37,38], that might be done by combining different omic strategies altogether as suggested by Yang [39]. On the other hand, Gale et al [40] explored the possibility of building a dose-response model for RNA virus but they could not succeed in having a quantitative estimation of probability of infection on the basis of omic data.…”

Section: Virulence and Hostpathogen Interactionsmentioning

confidence: 99%

Latest developments in foodborne pathogen risk assessment

Membré

Guillou

2016

Current Opinion in Food Science

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The adaptive response of bacterial food-borne pathogens in the environment, host and food: Implications for food safety

Cited by 101 publications

References 116 publications

Comparison of methods for the identification and sub-typing of O157 and non-O157 Escherichia coli serotypes and their integration into a polyphasic taxonomy approach

Comparison of methods for the identification and sub-typing of O157 and non-O157 Escherichia coli serotypes and their integration into a polyphasic taxonomy approach

Larvicidal evaluation of the Origanum majorana L. Essential Oil against the larvae of the Aedes aegypti mosquito

Latest developments in foodborne pathogen risk assessment

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