The activities of GST isozymes in stomach tissues of female obese patients

Abstract

ObjectivesObesity has become an important public health problem because of its increasing prevalence and relation with many diseases and mortality. Studies have shown its association with oxidative stress. In this study, the effect of obesity on total amount of thiol and some glutathione S-transferase (GST) isozymes were investigated which could serve as an important criteria in dose adjustment of some certain drugs in obese.MethodsThe gastric tissues removed by gastrectomy operation from 29 morbid obese female patients were analysed for thiol levels and activities of total GST, GSTT1-1 and GSTM1-1. Patients were grouped according to age, presence of hypertension and/or diabetes, and family history.ResultsThe average total thiol was 131.22 (±7.74) nmol/mg protein with no significant differences in between the groups. GSTT1 specific activities were about 20% higher in four groups: with ages over 35 years old, with hypertension, without diabetes and finally without family history, with respect to other groups. The differences between total GST and GSTM1 activity levels of experimental groups were not significant.ConclusionsThis is the first study to compare activities of GST isozymes and total thiol content in the stomach tissues of obese female patients accompanying some common metabolic disorders, age and family history.

An Assessment of Glutathione-S-Transferase and Lipid Profile in Obese Iraqi Patients

“…Another study [27] showed a highly significant BMI with obesity. According to the GST results, there is a substantial difference in GST between these groups and obesity; these findings are consistent with earlier studies [28].…”

Bisphenol S leads to cytotoxicity-induced antioxidant responses and oxidative stress in isolated rainbow trout (Oncorhyncus mykiss) hepatocytes

“…The reaction was started through the addition of 25 µL of the sample, which comprised 33.28-73.4 µg per well, and the absorbance data were obtained at 25°C, for 10 min, at 340 nm The contents of the reaction medium for the GSTT1-1 isozyme activity measurements were 0.1 M of phosphate buffer at a pH of 6.5, 0.25 mM of 1,2 -epoxy-3-(p-nitrophenoxy)-propane, and 0.5 mM of GSH. The reaction was started through the addition of 100 µL of the sample, which comprised 133.2-293.6 µg per well, and the absorbance data were obtained at 360 nm, for 10 min, at 25°C [32]. Speci c activity was calculated by using Eq.…”