The Absolute Basophil Count

Shelley, Walter B.; Parnes, Herbert M.

doi:10.1001/jama.1965.03080180026005

Cited by 31 publications

(5 citation statements)

References 5 publications

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“…Low precision of the analytical method particularly affects results in the lower range (42) and broadens the normal range (21). Reference ranges for basophils established with the more precise counting-chamber methods are in good agreement with our results (15,38,46). In summary, the described mAb-based flow cytometric differential showed an excellent basophil counting performance, recommending its use as basophil reference method or in clinical studies.…”

Section: Precisionsupporting

confidence: 87%

“…Although basophils are sometimes thought to be of minor clinical importance, we attach a great value to basophil counting performance. The basophil count has been shown to be altered in allergic reactions (14,46), including occupational opiate exposure (4) and oral nickelchrome alloy prosthesis (1), and to be increased in colitis ulcerosa (28), and it has long been known to be increased from the early stages of chronic myelogenous leukemia. A reliable basophil count could be useful in diagnosing or monitoring some of these conditions.…”

Section: Basophilsmentioning

confidence: 99%

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Toward a new reference method for the leukocyte five-part differential

et al. 1997

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A flow cytometric method performing a five‐part leukocyte differential based on three‐color staining with anti‐CD45‐fluorescein isothiocyanate (FITC), anti‐CD‐14‐phycoerythrin (PE)/Cy5, and a cocktail of PE‐labeled anti‐CD2, anti‐CD16, and anti‐HLA‐DR antibodies was evaluated. Results obtained by using three different sample preparation procedures and two different flow cytometers were compared with those of a 1,000‐cell manual differential for evaluation of accuracy. We observed excellent correlations with the manual differential for all leukocyte subclasses and even higher correlations between the different flow cytometric methods. Flow cytometric basophil results were identical to the manual counts, regardless of which sample preparation technique or flow cytometer was used. Therefore, we propose our flow cytometric method as the first acceptable automated reference method for basophil counting. The flow cytometric results for the other leukocyte subclasses were apparently influenced by the sample preparation, which could not be explained by cell loss during washing steps. Moreover, a small influence of the flow cytometer was also observed. Assessing the influence of sample storage, we found only minimal changes within 24 h. In establishing reference values, high precision of flow cytometric results facilitated detection of a significantly higher monocyte count for males (relative count: 7.08 ± 1.73% vs. 6.44 ± 1.33%, P < 0.05; absolute count: 0.536 ± 0.181 × 109/liter vs. 0.456 ± 139 × 109/liter, P < 0.01). Our data indicate that monoclonal antibody‐based flow cytometry is a highly suitable reference method for the five‐part differential: It also shows, however, that studies will have to put more emphasis on methodological issues to define a method that shows a high interlaboratory reproducibility. Cytometry 30:72–84, 1997. © 1997 Wiley‐Liss, Inc.

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Section: Precisionsupporting

confidence: 87%

Section: Basophilsmentioning

confidence: 99%

Toward a new reference method for the leukocyte five-part differential

et al. 1997

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“…So far, this had not been reported in malaria; in the literature, this reduction has been described in association with depression, 34 urticaria, 35 bladder cancer, 36 hyperthyroidism, and allergy. 37 …”

Section: Resultsmentioning

confidence: 99%

New laboratory perspectives for evaluation of vivax malaria infected patients: a useful tool for infection monitoring

Alves-Junior

Gomes

Dombroski

et al. 2020

The Brazilian Journal of Infectious Diseases

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“…This was largely independent of prior cancer therapy, except for one patient who had received recent prolonged, high-dose corticosteroids prior to sampling and whose blood was depleted of basophils. Although a previous study demonstrated that ex vivo incubation of blood samples with prednisolone for 30 min did not significantly alter anti-IgE-mediated basophil activation [ 31 ], reduced basophil counts in blood following systemic corticosteroid treatment have been reported [ 66 , 67 , 68 ]. However, our study is the first to consider the impact of prolonged, systemic corticosteroid therapy on the circulating basophils from cancer patients.…”

Section: Discussionmentioning

confidence: 99%

Basophils from Cancer Patients Respond to Immune Stimuli and Predict Clinical Outcome

Bax

Chauhan

Stavraka

et al. 2020

Cells

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Basophils are involved in manifestations of hypersensitivity, however, the current understanding of their propensity for activation and their prognostic value in cancer patients remains unclear. As in healthy and atopic individuals, basophil populations were identified in blood from ovarian cancer patients (n = 53) with diverse tumor histologies and treatment histories. Ex vivo basophil activation was measured by CD63 expression using the basophil activation test (BAT). Irrespective of prior treatment, basophils could be activated by stimulation with IgE- (anti-FcεRI and anti-IgE) and non-IgE (fMLP) mediated triggers. Basophil activation was detected by ex vivo exposure to paclitaxel, but not to other anti-cancer therapies, in agreement with a clinical history of systemic hypersensitivity reactions to paclitaxel. Protein and gene expression analyses support the presence of basophils (CCR3, CD123, FcεRI) and activated basophils (CD63, CD203c, tryptase) in ovarian tumors. Greater numbers of circulating basophils, cells with greater capacity for ex vivo stimulation (n = 35), and gene signatures indicating the presence of activated basophils in tumors (n = 439) were each associated with improved survival in ovarian cancer. Circulating basophils in cancer patients respond to IgE- and non-IgE-mediated signals and could help identify hypersensitivity to therapeutic agents. Activated circulating and tumor-infiltrating basophils may be potential biomarkers in oncology.

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The Absolute Basophil Count

Cited by 31 publications

References 5 publications

Toward a new reference method for the leukocyte five-part differential

Toward a new reference method for the leukocyte five-part differential

New laboratory perspectives for evaluation of vivax malaria infected patients: a useful tool for infection monitoring

Basophils from Cancer Patients Respond to Immune Stimuli and Predict Clinical Outcome

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